##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_80.341000000060fe.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 136600 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.38584187408492 38.0 38.0 38.0 32.0 38.0 2 36.38139824304539 38.0 38.0 38.0 32.0 38.0 3 36.3600878477306 38.0 38.0 38.0 32.0 38.0 4 36.44573206442167 38.0 38.0 38.0 32.0 38.0 5 36.47098096632504 38.0 38.0 38.0 32.0 38.0 6 38.38300878477306 40.0 38.0 40.0 38.0 40.0 7 38.273792093704245 40.0 38.0 40.0 38.0 40.0 8 38.25235724743777 40.0 38.0 40.0 38.0 40.0 9 38.26297218155198 40.0 38.0 40.0 38.0 40.0 10 38.20855783308931 40.0 38.0 40.0 38.0 40.0 11 38.1846486090776 40.0 38.0 40.0 38.0 40.0 12 38.12515373352855 40.0 38.0 40.0 38.0 40.0 13 38.1011859443631 40.0 38.0 40.0 38.0 40.0 14 38.01507320644217 40.0 38.0 40.0 32.0 40.0 15 38.037496339677894 40.0 38.0 40.0 32.0 40.0 16 38.020658857979505 40.0 38.0 40.0 32.0 40.0 17 38.08624450951684 40.0 38.0 40.0 38.0 40.0 18 38.073799414348464 40.0 38.0 40.0 38.0 40.0 19 38.13439238653002 40.0 38.0 40.0 38.0 40.0 20 38.12041727672035 40.0 38.0 40.0 38.0 40.0 21 38.14507320644217 40.0 38.0 40.0 38.0 40.0 22 38.13558565153733 40.0 38.0 40.0 38.0 40.0 23 38.10411420204978 40.0 38.0 40.0 38.0 40.0 24 38.122306002928255 40.0 38.0 40.0 38.0 40.0 25 38.121756954612 40.0 38.0 40.0 38.0 40.0 26 38.05234992679356 40.0 38.0 40.0 32.0 40.0 27 38.04737188872621 40.0 38.0 40.0 32.0 40.0 28 38.017049780380674 40.0 38.0 40.0 32.0 40.0 29 37.995234260614936 40.0 38.0 40.0 32.0 40.0 30 37.96677159590044 40.0 38.0 40.0 32.0 40.0 31 37.9639019033675 40.0 38.0 40.0 32.0 40.0 32 37.9463616398243 40.0 38.0 40.0 32.0 40.0 33 37.89641288433382 40.0 38.0 40.0 32.0 40.0 34 37.91672767203514 40.0 38.0 40.0 32.0 40.0 35 37.91776720351391 40.0 38.0 40.0 32.0 40.0 36 37.866266471449485 40.0 38.0 40.0 32.0 40.0 37 37.86103953147877 40.0 38.0 40.0 32.0 40.0 38 37.8260102489019 40.0 38.0 40.0 32.0 40.0 39 37.78734260614934 40.0 38.0 40.0 32.0 40.0 40 37.75240849194729 40.0 38.0 40.0 32.0 40.0 41 37.762445095168374 40.0 38.0 40.0 32.0 40.0 42 37.7037701317716 40.0 38.0 40.0 32.0 40.0 43 37.6905710102489 40.0 38.0 40.0 32.0 40.0 44 37.631581259150806 40.0 38.0 40.0 32.0 40.0 45 37.56990483162518 40.0 38.0 40.0 32.0 40.0 46 37.48407027818448 40.0 38.0 40.0 32.0 40.0 47 37.43527818448023 40.0 38.0 40.0 32.0 40.0 48 37.37705710102489 40.0 38.0 40.0 32.0 40.0 49 37.315387994143485 40.0 38.0 40.0 32.0 40.0 50 37.25797218155198 40.0 38.0 40.0 32.0 40.0 51 36.8849121522694 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.08819022050160186 2201 2 0.29693147469586734 2201 3 0.5202863921492877 2201 4 0.5549624065100076 2201 5 0.35775005686175376 2201 6 0.0598715271798369 2201 7 0.49807604660555427 2201 8 0.4236463867274267 2201 9 0.6529286939362606 2201 10 0.6988703600783097 2201 11 0.7433214678975162 2201 12 0.4099630331291806 2201 13 0.24330683143291054 2201 14 0.41384585513116434 2201 15 0.4186692211157492 2201 16 0.3226890710123982 2201 17 -0.09406173732579504 2201 18 0.1778884445733624 2201 19 0.5757399322071421 2201 20 0.28360666004444823 2201 21 0.3876679034392936 2201 22 0.5916392788080174 2201 23 0.40527464743495756 2201 24 0.21712540531510882 2201 25 0.5812154975146413 2201 26 -0.08431708836571516 2201 27 0.3949234170368925 2201 28 0.23355581032173944 2201 29 0.44000798090404203 2201 30 0.2862893435532854 2201 31 0.2963963890601349 2201 32 0.40961663137989035 2201 33 0.06675489695309977 2201 34 0.7407740474695217 2201 35 0.4367353333287909 2201 36 0.392218382843069 2201 37 0.9323369377644894 2201 38 0.39548394925390085 2201 39 0.14312474376031048 2201 40 0.28846088166279316 2201 41 0.3021726554137416 2201 42 0.16195511385844696 2201 43 0.6461680989652407 2201 44 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-1.028782175287688 1213 49 -1.2815779849030946 1213 50 -1.11786619086606 1213 51 -1.0129643417657022 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 5.0 17 4.0 18 1.0 19 5.0 20 10.0 21 22.0 22 58.0 23 100.0 24 191.0 25 333.0 26 546.0 27 788.0 28 1144.0 29 1549.0 30 1837.0 31 2364.0 32 2964.0 33 3523.0 34 4511.0 35 5540.0 36 7585.0 37 11214.0 38 20987.0 39 71318.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.434794071123086 22.783994375768938 13.539164567344308 24.242046985763665 2 12.483436678697188 24.30287634428282 40.850091876102695 22.363595100917298 3 20.08784773060029 27.848462664714496 30.1207906295754 21.94289897510981 4 12.664714494875549 20.50805270863836 37.17423133235725 29.65300146412884 5 12.666910688140556 36.789165446559295 35.896046852122986 14.64787701317716 6 30.181040856814473 39.46734602742333 16.22998704236488 14.121626073397318 7 22.306734992679356 35.45534407027818 21.902635431918007 20.33528550512445 8 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21 17.244509516837482 30.954612005856514 30.818448023426058 20.982430453879942 22 17.707174231332356 33.819912152269396 30.832357247437773 17.640556368960468 23 16.632503660322108 31.281844802342608 32.04685212298683 20.038799414348464 24 19.39311859443631 31.17862371888726 32.75622254758419 16.67203513909224 25 18.103953147877014 32.250366032210835 32.13177159590044 17.513909224011712 26 17.546120058565155 31.610541727672036 33.52415812591508 17.31918008784773 27 17.358711566617863 32.84333821376281 32.63836017569546 17.159590043923863 28 16.462664714494878 31.56515373352855 32.179355783308935 19.792825768667644 29 17.61493411420205 30.919472913616396 33.23206442166911 18.233528550512446 30 16.859328672352575 31.087521505179545 34.028331930158494 18.02481789230938 31 19.828696925329428 31.065885797950223 31.56954612005857 17.535871156661788 32 17.071742313323572 32.08199121522694 32.09590043923865 18.750366032210835 33 16.816983894582723 32.558565153733525 34.14714494875549 16.47730600292826 34 18.662518301610543 32.53294289897511 32.53294289897511 16.27159590043924 35 18.026354319180086 32.92240117130307 32.179355783308935 16.871888726207906 36 16.378477306002928 34.19180087847731 32.67349926793558 16.756222547584187 37 17.00878477306003 33.06954612005856 31.500732064421673 18.420937042459737 38 17.204245973645683 33.10102489019034 32.24816983894583 17.446559297218155 39 17.691068814055637 31.585651537335284 31.88433382137628 18.8389458272328 40 18.823572474377745 31.349194729136165 32.96046852122987 16.866764275256223 41 18.01610541727672 30.99121522693997 32.497071742313324 18.495607613469986 42 18.505124450951683 32.480234260614935 32.2086383601757 16.806002928257687 43 16.428257686676428 32.44582723279649 33.92679355783309 17.199121522693996 44 16.453147877013176 33.25402635431918 32.26427525622255 18.028550512445097 45 16.50805270863836 32.073938506588576 33.186676427525626 18.23133235724744 46 18.308931185944363 30.991947291361637 31.59224011713031 19.106881405563687 47 17.93338213762811 31.25622254758419 31.275988286969252 19.53440702781845 48 16.68740849194729 31.549780380673496 31.576134699853586 20.186676427525622 49 18.655197657393852 31.22254758418741 31.551244509516835 18.571010248901903 50 16.871156661786237 32.519765739385065 32.09297218155198 18.51610541727672 51 16.155056920092242 32.23983308320217 31.691496760496356 19.913613236209233 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1291.0 1 749.5 2 208.0 3 412.5 4 617.0 5 587.5 6 558.0 7 724.0 8 890.0 9 731.5 10 573.0 11 1000.5 12 1428.0 13 2511.0 14 3594.0 15 3944.0 16 4294.0 17 3144.5 18 1995.0 19 1646.5 20 1298.0 21 2072.0 22 2846.0 23 1979.0 24 1112.0 25 1207.5 26 2224.0 27 3145.0 28 2971.5 29 2798.0 30 4235.0 31 5672.0 32 7575.0 33 9478.0 34 10710.0 35 11942.0 36 11914.0 37 11886.0 38 10851.0 39 9816.0 40 9016.0 41 8216.0 42 7553.5 43 6891.0 44 6513.5 45 6136.0 46 5965.5 47 5795.0 48 5665.0 49 5535.0 50 5545.0 51 5555.0 52 6004.5 53 6454.0 54 5196.0 55 3938.0 56 3580.0 57 3222.0 58 2897.5 59 2573.0 60 2233.0 61 1893.0 62 1609.0 63 1325.0 64 1122.0 65 919.0 66 740.5 67 562.0 68 453.5 69 345.0 70 278.5 71 212.0 72 172.0 73 132.0 74 106.5 75 62.0 76 43.0 77 27.5 78 12.0 79 10.5 80 9.0 81 8.0 82 7.0 83 4.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03513909224011713 2 0.0021961932650073207 3 0.0 4 0.0 5 0.0 6 7.320644216691069E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 7.320644216691069E-4 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0014641288433382138 17 0.0 18 7.320644216691069E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0036603221083455345 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0036603221083455345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 136600.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.51184780234404 #Duplication Level Percentage of deduplicated Percentage of total 1 89.88526948572697 64.27861711135984 2 7.112721200829972 10.172876719485172 3 1.5450209503106405 3.3146190915014238 4 0.5533208549159501 1.582759870504492 5 0.22400142416917038 0.800937787634701 6 0.16713403917302144 0.7171238383159269 7 0.09958871171424305 0.4985240956458325 8 0.066960819548058 0.3830793548992758 9 0.05300221116978045 0.3411257452524934 >10 0.2187464185943408 3.0262806072916533 >50 0.03472051117722785 1.8284737307198433 >100 0.02836912313056493 4.414515252168746 >500 0.007091795161856986 3.7065228242096513 >1k 0.004052454378203993 4.934543971010934 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2425 1.7752562225475843 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 1800 1.3177159590043925 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1359 0.9948755490483163 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1227 0.8982430453879942 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 996 0.7291361639824304 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 983 0.7196193265007321 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 941 0.6888726207906296 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 579 0.4238653001464128 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 577 0.4224011713030747 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 526 0.38506588579795026 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 514 0.3762811127379209 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 417 0.3052708638360176 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 389 0.28477306002928254 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 384 0.28111273792093705 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 347 0.2540263543191801 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 340 0.24890190336749632 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 310 0.2269399707174231 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 293 0.21449487554904834 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 280 0.20497803806734993 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 256 0.18740849194729137 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 254 0.18594436310395315 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 220 0.16105417276720352 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 218 0.1595900439238653 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 217 0.1588579795021962 No Hit TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT 206 0.15080527086383602 Illumina PCR Primer Index 8 (95% over 21bp) GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 189 0.1383601756954612 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 186 0.13616398243045388 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 177 0.1295754026354319 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 163 0.11932650073206442 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 157 0.11493411420204977 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 140 0.10248901903367497 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 137 0.10029282576866765 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0014641288433382138 3 0.0 0.0 0.0021961932650073207 4 0.0 0.0 0.0021961932650073207 5 0.0 0.0 0.0021961932650073207 6 0.0 0.0 0.0021961932650073207 7 0.0 0.0 0.0021961932650073207 8 0.0 0.0 0.0021961932650073207 9 0.0 0.0 0.0036603221083455345 10 0.0 0.0 0.0036603221083455345 11 0.0 0.0 0.0036603221083455345 12 0.0 0.0 0.004392386530014641 13 0.0 0.0 0.004392386530014641 14 0.0 0.0 0.004392386530014641 15 0.0 0.0 0.004392386530014641 16 0.0 0.0 0.005124450951683748 17 0.0 0.0 0.005124450951683748 18 0.0 0.0 0.005124450951683748 19 0.0 0.0 0.005856515373352855 20 0.0 0.0 0.007320644216691069 21 0.0 0.0 0.008784773060029283 22 0.0 0.0 0.01171303074670571 23 0.0 0.0 0.01683748169838946 24 0.0 0.0 0.029282576866764276 25 0.0 0.0 0.0314787701317716 26 0.0 0.0 0.04538799414348463 27 0.0 0.0 0.04758418740849195 28 0.0 0.0 0.06808199121522694 29 0.0 0.0 0.0951683748169839 30 0.0 0.0 0.11932650073206442 31 0.0 0.0 0.23718887262079064 32 0.0 0.0 0.35871156661786235 33 0.0 0.0 0.4773060029282577 34 0.0 0.0 0.6559297218155198 35 0.0 0.0 0.8177159590043924 36 0.0 0.0 1.0571010248901904 37 0.0 0.0 1.3155197657393851 38 0.0 0.0 1.644216691068814 39 0.0 0.0 2.0219619326500733 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGT 235 0.0 39.255 37 CGTATGC 235 0.0 39.255 41 CTCGTAT 235 0.0 39.255 39 TCGTATG 235 0.0 39.255 40 CACGAGA 240 0.0 38.437183 21 TATGCCG 240 0.0 38.437183 43 TCTCGTA 240 0.0 38.437183 38 ATGCCGT 240 0.0 38.437183 44 CGAGACT 235 0.0 38.29756 23 GCCCACG 245 0.0 37.652752 18 CCCACGA 245 0.0 37.652752 19 CGAGCCC 245 0.0 37.652752 15 ACGAGAC 245 0.0 37.652752 22 CCACGAG 245 0.0 37.652752 20 TGCCGTC 245 0.0 37.652752 45 GGAATCT 240 0.0 37.49969 34 GACTTGC 235 0.0 37.340122 26 GTATGCC 250 0.0 36.899696 42 CCGAGCC 250 0.0 36.899696 14 TCTCCGA 250 0.0 36.899696 11 >>END_MODULE