H23NVBCXX l02n01 36h_8.34100000005bea.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 36h_8.34100000005bea.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41503
Sequences flagged as poor quality	0
Sequence length	51
%GC	34

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2464	5.936920222634509	No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTT	2336	5.628508782497651	No Hit
ATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTT	1170	2.8190733200009634	No Hit
GATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT	962	2.3179047297785704	No Hit
CTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT	873	2.1034624003084113	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	787	1.8962484639664605	RNA PCR Primer, Index 27 (95% over 21bp)
TGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTT	715	1.722767028889478	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	640	1.5420572006842879	No Hit
ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACT	575	1.3854420162397898	No Hit
GTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT	479	1.1541334361371467	No Hit
TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	460	1.1083536129918319	No Hit
CTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	442	1.0649832542225863	No Hit
CCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTT	382	0.9204153916584343	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	346	0.8336746741199431	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	329	0.7927137797267667	No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCTGTCTCTTATACACATCT	243	0.5854998433848155	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCC	222	0.5349010914873623	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	218	0.5252632339830855	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGA	200	0.48189287521383994	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCCTG	190	0.45779823145314796	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCA	187	0.45056983832494035	No Hit
TACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	185	0.44575090957280195	No Hit
TTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT	174	0.41924680143604076	No Hit
ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCCTGTCTCT	155	0.373466978290726	No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCCTGTCTCTTATACACATC	154	0.3710575139146568	No Hit
CTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTT	143	0.34455340577789556	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	141	0.33973447702575715	No Hit
GCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	130	0.31323036888899597	No Hit
GCGTTGATACCACTGCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT	128	0.30841144013685756	No Hit
ATACCACTGCTTGCAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCT	124	0.29877358263258075	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	124	0.29877358263258075	No Hit
CGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTT	118	0.2843167963761656	No Hit
CACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT	116	0.2794978676240272	No Hit
CTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTT	112	0.26986001011975036	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	109	0.26263161699154275	No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCACTGTCTCTTATACACAT	101	0.24335590198298918	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCTGTCTC	94	0.22648965135050478	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93	0.22408018697443557	No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAGTACTCTGCGTTGATACCACT	93	0.22408018697443557	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCTGTCTCTT	93	0.22408018697443557	No Hit
ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCTGTCTCTTATACA	89	0.21444232947015876	No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT	89	0.21444232947015876	RNA PCR Primer, Index 38 (95% over 21bp)
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACAC	88	0.21203286509408956	No Hit
ATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTC	85	0.20480447196588197	No Hit
GCGTTGATACCACTGCTTGCAAGCAGCTGTCTCTTATACACATCTCCGAGC	85	0.20480447196588197	No Hit
GCTTGCAAGCAGTGGTATCTGTCTCTTATACACATCTCCGAGCCCACGAGA	85	0.20480447196588197	No Hit
CAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	81	0.1951666144616052	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCCTGTCTCTTATACAC	78	0.18793822133339758	No Hit
GCTTGCAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCTCCGAGCCC	76	0.1831192925812592	No Hit
TGCAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCTCCGAGCCCACG	75	0.18070982820519	No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAAC	74	0.1783003638291208	No Hit
GCTTGCAAGCAGTGGTATCCTGTCTCTTATACACATCTCCGAGCCCACGAG	73	0.17589089945305159	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	73	0.17589089945305159	No Hit
ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCCTGTCTCTTATAC	73	0.17589089945305159	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	69	0.1662530419487748	No Hit
GTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTA	69	0.1662530419487748	No Hit
GCTTGCAAGCAGTGGTATCACTGTCTCTTATACACATCTCCGAGCCCACGA	68	0.1638435775727056	No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGCTGTCTCTTATACACATCTCCG	67	0.1614341131966364	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCCTGTCT	67	0.1614341131966364	No Hit
GTTGATACCACTGCTTGCAAGCCTGTCTCTTATACACATCTCCGAGCCCAC	67	0.1614341131966364	No Hit
GCGTTGATACCACTGCTTGCAAGCCTGTCTCTTATACACATCTCCGAGCCC	65	0.15661518444449798	No Hit
CTTGCAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCTCCGAGCCCA	64	0.15420572006842878	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCACTGTCTC	64	0.15420572006842878	No Hit
CTGCTTGCAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCTCCGAGC	64	0.15420572006842878	No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGC	63	0.15179625569235958	No Hit
GCTTGCAAGCAGTGGTATCAACCTGTCTCTTATACACATCTCCGAGCCCAC	63	0.15179625569235958	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTCCAAGCAGTGGTATCAACGCA	62	0.14938679131629037	No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTC	61	0.1469773269402212	No Hit
ATACCACTGCTTGCAAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA	60	0.144567862564152	No Hit
CTGCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	60	0.144567862564152	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTCCAAGCAGTGGTATCAACGCAGAG	59	0.1421583981880828	No Hit
ATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTT	59	0.1421583981880828	No Hit
GATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCT	58	0.1397489338120136	No Hit
ATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTCTGTCTCTTATACA	58	0.1397489338120136	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	58	0.1397489338120136	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCCTGTCTCT	57	0.13733946943594438	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCACTGTC	57	0.13733946943594438	No Hit
ACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT	56	0.13493000505987518	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56	0.13493000505987518	No Hit
GATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCT	55	0.13252054068380598	No Hit
CAGTGGTATCAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAT	54	0.13011107630773677	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54	0.13011107630773677	No Hit
CTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATAC	51	0.12288268317952919	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACCTG	50	0.12047321880345999	No Hit
TGCAAGCAGTGGTATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGA	49	0.11806375442739078	No Hit
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACAT	49	0.11806375442739078	No Hit
GCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	49	0.11806375442739078	No Hit
TGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTC	49	0.11806375442739078	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTCCAAGCAGTGGTATCAACG	47	0.11324482567525239	No Hit
GTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCCTGTCT	47	0.11324482567525239	No Hit
GATACCACTGCTTGGAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTG	47	0.11324482567525239	No Hit
TGATACCACTGCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTT	47	0.11324482567525239	No Hit
GATACCACTGCTTGCAAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG	46	0.11083536129918319	No Hit
CCACTGCTTGCAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCTCCG	45	0.10842589692311398	No Hit
ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCACTGTCTCTTATA	45	0.10842589692311398	No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACG	45	0.10842589692311398	No Hit
TGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT	45	0.10842589692311398	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCCTGTCTCTTATACACAT	42	0.10119750379490638	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATCT	30	2.1151736E-6	45.000004	34
CGATAAC	25	3.8259743E-5	45.000004	28
GATAACC	25	3.8259743E-5	45.000004	29
AACCTAT	25	3.8259743E-5	45.000004	32
ATAACCT	25	3.8259743E-5	45.000004	30
TAACCTA	25	3.8259743E-5	45.000004	31
AGACGAT	25	3.8259743E-5	45.000004	25
GACGATA	25	3.8259743E-5	45.000004	26
ACCTATC	30	2.1151736E-6	45.000004	33
ACGATAA	25	3.8259743E-5	45.000004	27
GTATGCC	150	0.0	42.0	42
ATCTCGT	150	0.0	42.0	37
TGCCGTC	150	0.0	42.0	45
TATGCCG	150	0.0	42.0	43
TATCTCG	150	0.0	42.0	36
ATGCCGT	150	0.0	42.0	44
CGTATGC	150	0.0	42.0	41
TCGTATG	150	0.0	42.0	40
CTCGTAT	150	0.0	42.0	39
CTATCTC	140	0.0	41.785717	35

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content