FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_79.341000000060e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_79.341000000060e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178061
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC79394.458584417699552No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC15740.8839667305024683No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC14640.8221901483199577No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACA10380.5829462936858717No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA9360.5256625538439075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6820.383014809531565No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC6360.3571809660734243No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACATC6210.3487568866849001No Hit
TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA5700.32011501676391796No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG5680.3189918061787814No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACA5630.31618377971594No Hit
CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG5370.30158204210916484No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACA4840.27181696160304614No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT4710.26451609279965854Illumina PCR Primer Index 8 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT4630.26002325045911234No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC4530.25440719753342955No Hit
TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA3990.22408051173474258No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC3920.22014927468676465No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCC3750.21060198471310396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.2089171688353991No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.2021779053245798No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCC3280.1842065359623949No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACATC3150.1769056671590073No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACATC3080.17297443011102936No Hit
TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC3050.17128961423332453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.17072800894075627No Hit
TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC2790.15668787662654932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.15500306074884448No Hit
TTTGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA2710.15219503428600312No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCAAACTGTCTCTTATACACA2690.15107182370086655No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA2490.13983971784950103No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCAAACTGTCTCTTATACACATC2230.1252379802427258No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.11625229556163337No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.11400587439136026No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTC2010.11288266380622371No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAACTGTCTCTTATACAC1990.11175945322108714No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1880.1055817950028361No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGA1850.10389697912513128No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.10333537383256299No Hit
AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA1790.1005273473697216No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8900.043.73404745
ATGCCGT9400.041.40776444
AATCTCG9350.040.90730736
TATGCCG9550.040.7573843
TCTCGTA9550.040.7573838
CTCGTAT9600.040.54510539
CACGAGA9600.040.54510521
GTATGCC9600.040.54510542
ACGAGAC9600.040.54510522
ATCTCGT9550.040.5217937
CCACGAG9550.040.5217920
TCGTATG9650.040.33502640
CGAGACT9600.040.07637823
GGAATCT9600.039.8420134
CGTATGC9800.039.7176541
GCCCACG9750.039.70172518
CCCACGA9750.039.6905819
TTGCTGG9650.039.63557429
TGGAATC9600.039.60764733
GAATCTC9700.039.43126735
Produced by FastQC (version 0.11.2)