FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_78.341000000060d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_78.341000000060d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248975
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC18050.7249723867858219No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA6450.2590621548348228No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT3540.14218295009539111No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC2820.1132643839742946No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG2200.038.86207636
CTCGTAT2350.038.29633339
TATGCCG2350.038.29633343
TCGTATG2350.038.29633340
ATGCCGT2350.038.29633344
GCCCACG2400.037.49849318
TCTCGTA2400.037.49849338
CCACGAG2400.037.49849320
TGCCGTC2400.037.49849345
CACGAGA2450.036.7332221
CCCACGA2450.036.7332219
ACGAGAC2450.036.7332222
CGAGACT2400.036.5610323
CGAGCCC2650.033.9677215
ATCTCGT2650.033.960937
CGTATGC2650.033.960941
CCGAGCC2750.032.73253314
GACTTGC2700.032.49869526
AGCCCAC2850.032.36712317
GTATGCC2850.031.57767942
Produced by FastQC (version 0.11.2)