FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_77.341000000060c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_77.341000000060c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257428
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC30581.187904967602592No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA2880.11187594201097005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4100.039.5195345
ATCTCGT3950.039.3034637
TATGCCG4150.039.0358143
AATCTCG3900.038.65351536
CCCACGA4250.038.11731719
ATGCCGT4250.038.11731744
GCCCACG4300.037.67409518
ACGAGAC4300.037.67409522
TCTCGTA4150.037.40931738
CGAGACT4250.037.05850623
CTCGTAT4200.036.96396639
CGTATGC4400.036.81786341
CCACGAG4400.036.81786320
CACGAGA4450.036.4041821
CCGAGCC4450.036.4041814
TCGTATG4450.036.4041840
GACTTGC4300.036.1043426
GTATGCC4550.035.60408842
TCCGAGC4750.034.5786513
TTGCTGG4500.034.49970229
Produced by FastQC (version 0.11.2)