FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_76.341000000060ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_76.341000000060ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157513
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC19501.2379930545415299No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.41964790207792374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6580.41774329737862903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.22918743214845758No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.19998349342593946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.18093744643299284No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT2680.17014468647032308No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1770.11237167725838502No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1690.10729273139359927No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT1640.10411839022810816Illumina PCR Primer Index 8 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2050.039.51191745
CGAGACT2150.038.7206623
ATGCCGT2100.038.57115644
GCCCACG2200.037.8406418
TCCGAGC2200.037.8406413
CACGAGA2200.037.8406421
CCCACGA2200.037.8406419
CGAGCCC2200.037.8406415
ACGAGAC2200.037.8406422
CCACGAG2200.037.8406420
ATCTCGT2150.037.67415637
CTCGTAT2150.037.67415639
TATGCCG2150.037.67415643
TCTCGTA2150.037.67415638
AATCTCG2150.037.67415636
GCCGTCT301.1355021E-437.49973345
CCGAGCC2250.036.9997414
TCGTATG2250.035.9997540
CCAGTCT250.002101676835.99974413
AGACTTG2400.035.6247525
Produced by FastQC (version 0.11.2)