FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_75.341000000060ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_75.341000000060ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205407
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC20270.98682128651896No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA2920.1421567911512266No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT2200.10710443168928031No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2350.039.25425743
CCCACGA2350.039.25425719
CGAGACT2350.039.25425723
TGCCGTC2400.038.4364645
GCCCACG2450.037.65204218
CTCGTAT2450.037.65204239
CCGAGCC2450.037.65204214
CGAGCCC2450.037.65204215
ACGAGAC2450.037.65204222
CCACGAG2450.037.65204220
ATCTCGT2500.036.89900237
CACGAGA2550.036.1754921
TCTCGTA2550.036.1754938
AATCTCG2550.036.1754936
ATAGGTC250.002103369735.9990273
GACTTGC2500.035.99902326
TCCGAGC2600.035.4798113
TCGTATG2600.035.4798140
ATCTCCG2650.034.8103810
CTCCGAG2650.034.8103812
Produced by FastQC (version 0.11.2)