FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_74.34100000006091.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_74.34100000006091.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301780
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC9470.31380475843329575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1550.036.29005443
AATCTCG1550.036.29005436
ATCTCGT1650.034.09065637
ATGCCGT1650.034.09065644
TCTCCGA1750.033.42832611
GCCCACG1700.033.0879918
CTCGTAT1700.033.0879939
TCTCGTA1700.033.0879938
ACGAGAC1700.033.0879922
CACGAGA1750.032.1426221
CCCACGA1750.032.1426219
TGCCGTC1700.031.7644745
CGTATGC1800.031.24977141
CCGAGCC1800.031.24977114
TCGTATG1850.030.4051840
CGAGACT1850.030.4051823
TCCGAGC1900.029.60504513
GAATCTC1850.029.18897235
GTATGCC1950.028.8459442
CGAGCCC1950.028.8459415
Produced by FastQC (version 0.11.2)