FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_72.3410000000606a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_72.3410000000606a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195540
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC25081.2826020251610923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8600.43980771197708907No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5580.2853636084688555No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA5240.2679758617162729No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.25825917970747675No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT4610.23575738979236985No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.18666257543213666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.16364938120077735No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3140.16058095530326277No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC2830.14472742149943746No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA2660.13603354812314616No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT2540.129896696328117Illumina PCR Primer Index 11 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.10228086325048584No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT1970.10074665030172854No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACGA2450.040.40632624
CGCTATA2400.040.31066531
ACGCTAT2400.040.31066530
GACGACG2400.040.31066526
CGAGACG2500.039.598223
CGACGCT2450.039.48800328
ACGACGC2450.039.48800327
TCGTATG2600.038.9405440
ATGCCGT2600.038.9405444
AGACGAC2500.038.69824225
TATCTCG2500.038.69824236
GACGCTA2500.038.69824229
TATGCCG2650.038.20580743
CCACGAG2650.038.20580720
TGCCGTC2650.038.20580745
CTCGTAT2600.038.0751939
TCTCGTA2600.038.0751938
CTATATC2500.037.79828333
GCCCACG2700.037.4982918
CCCACGA2700.037.4982919