##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_71.3410000000605e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 56575 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.254670790985415 38.0 38.0 38.0 32.0 38.0 2 36.322845779938135 38.0 38.0 38.0 32.0 38.0 3 36.27485638532921 38.0 38.0 38.0 32.0 38.0 4 36.35006628369421 38.0 38.0 38.0 32.0 38.0 5 36.406115775519226 38.0 38.0 38.0 32.0 38.0 6 38.23902783915157 40.0 38.0 40.0 38.0 40.0 7 38.13753424657534 40.0 38.0 40.0 38.0 40.0 8 38.11833848873177 40.0 38.0 40.0 38.0 40.0 9 38.10746796288113 40.0 38.0 40.0 38.0 40.0 10 38.03262925320371 40.0 38.0 40.0 32.0 40.0 11 38.015271763146266 40.0 38.0 40.0 32.0 40.0 12 37.99618205921343 40.0 38.0 40.0 32.0 40.0 13 38.01037560760053 40.0 38.0 40.0 32.0 40.0 14 37.906637207247016 40.0 38.0 40.0 32.0 40.0 15 37.919664162615994 40.0 38.0 40.0 32.0 40.0 16 37.935802032699954 40.0 38.0 40.0 32.0 40.0 17 37.952788334069815 40.0 38.0 40.0 32.0 40.0 18 37.93189571365444 40.0 38.0 40.0 32.0 40.0 19 37.97843570481662 40.0 38.0 40.0 32.0 40.0 20 37.993654441007514 40.0 38.0 40.0 32.0 40.0 21 38.03701281484755 40.0 38.0 40.0 32.0 40.0 22 37.99342465753425 40.0 38.0 40.0 32.0 40.0 23 37.9307644719399 40.0 38.0 40.0 32.0 40.0 24 37.972832523199294 40.0 38.0 40.0 32.0 40.0 25 37.89905435262925 40.0 38.0 40.0 32.0 40.0 26 37.84979231109147 40.0 38.0 40.0 32.0 40.0 27 37.78815731330093 40.0 38.0 40.0 32.0 40.0 28 37.76309323906319 40.0 38.0 40.0 32.0 40.0 29 37.68086610693769 40.0 38.0 40.0 32.0 40.0 30 37.63793194874061 40.0 38.0 40.0 32.0 40.0 31 37.639858594785686 40.0 38.0 40.0 32.0 40.0 32 37.5736809544852 40.0 38.0 40.0 32.0 40.0 33 37.533079982324345 40.0 38.0 40.0 32.0 40.0 34 37.487635881573134 40.0 38.0 40.0 32.0 40.0 35 37.44896155545736 40.0 38.0 40.0 32.0 40.0 36 37.37700397702165 40.0 38.0 40.0 32.0 40.0 37 37.31920459566947 40.0 38.0 40.0 32.0 40.0 38 37.27787892178524 40.0 38.0 40.0 32.0 40.0 39 37.22379142730888 40.0 38.0 40.0 32.0 40.0 40 37.21746354396819 40.0 38.0 40.0 32.0 40.0 41 37.19554573574901 40.0 38.0 40.0 32.0 40.0 42 37.14212991604065 40.0 38.0 40.0 32.0 40.0 43 37.13246133451171 40.0 38.0 40.0 32.0 40.0 44 37.06198851082634 40.0 38.0 40.0 32.0 40.0 45 37.04258064516129 40.0 38.0 40.0 32.0 40.0 46 36.97583738400353 40.0 38.0 40.0 32.0 40.0 47 36.932850198851085 40.0 38.0 40.0 32.0 40.0 48 36.886221829429964 40.0 38.0 40.0 32.0 40.0 49 36.831144498453384 40.0 38.0 40.0 32.0 40.0 50 36.782235969951394 40.0 38.0 40.0 32.0 40.0 51 36.21836500220946 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.05099612952906085 2201 2 0.4865065993341986 2201 3 0.3454018099618352 2201 4 0.2057054127811071 2201 5 1.0133024539755553 2201 6 0.7513288348577163 2201 7 -0.044493926668423 2201 8 0.03815710849583098 2201 9 -0.37390350097226843 2201 10 -0.02786046738986414 2201 11 0.763708606526194 2201 12 0.4380284185356871 2201 13 -0.0037115165569190367 2201 14 0.19825788359789698 2201 15 0.3304393703160571 2201 16 0.7868243295937418 2201 17 -0.18355594334454395 2201 18 0.6882030811789193 2201 19 0.710773042147899 2201 20 0.490633703307374 2201 21 -0.12175506904861066 2201 22 0.21187369833145908 2201 23 0.2431098623718455 2201 24 -0.4445620956627536 2201 25 -0.038965102829799036 2201 26 0.7726486130845132 2201 27 0.4768248453208557 2201 28 0.25329600564678145 2201 29 0.29620666693583786 2201 30 0.16760710354754593 2201 31 0.5258648779262174 2201 32 0.4817902915315031 2201 33 0.06559392529111818 2201 34 0.24420808438996744 2201 35 0.5247284131245635 2201 36 0.9352420034013562 2201 37 1.1061953678408187 2201 38 0.3637046476886425 2201 39 0.3533288255731364 2201 40 1.293543089395179 2201 41 0.35128992075840415 2201 42 0.3857272356276269 2201 43 -0.2914175452107912 2201 44 0.5600903972392572 2201 45 0.5434156614020793 2201 46 0.1521695908739531 2201 47 0.007115222378601516 2201 48 -0.4375896388903726 2201 49 0.85879103463121 2201 50 0.8790437267942934 2201 51 0.42064366339453585 1101 1 0.11567053713761055 1101 2 0.4170621548897486 1101 3 0.23429069885072096 1101 4 -0.05818347610778574 1101 5 0.5133024539755553 1101 6 0.9318843904132734 1101 7 0.46939496222046984 1101 8 0.39926821960694525 1101 9 0.7510964990277316 1101 10 1.2221395326101359 1101 11 1.1525974954150797 1101 12 -0.020304914797641516 1101 13 1.0240662612208595 1101 14 0.5315912169312256 1101 15 0.24710603698272138 1101 16 0.772935440704849 1101 17 0.14977738998879175 1101 18 0.5076475256233621 1101 19 0.6135508199256776 1101 20 0.21285592552959542 1101 21 0.7532449309513893 1101 22 0.7674292538870091 1101 23 0.8819987512607383 1101 24 0.04154901544836065 1101 25 0.7388126749479795 1101 26 0.8837597241956274 1101 27 0.2823804008764057 1101 28 0.58662933898011 1101 29 -0.24545999973083354 1101 30 1.2370515479919888 1101 31 0.6369759890373246 1101 32 -0.7404319306907183 1101 33 0.4822605919577896 1101 34 1.1192080843899674 1101 35 0.4275061909023421 1101 36 0.2963531145124705 1101 37 1.0367509233963759 1101 38 0.41926020324419966 1101 39 1.450551047795365 1101 40 1.5852097560618432 1101 41 1.3096232540917399 1101 42 1.4135050134054055 1101 43 0.6669157881225445 1101 44 0.9767570639059215 1101 45 1.1684156614020793 1101 46 0.9160584797628459 1101 47 1.3821152223786015 1101 48 1.1874103611096274 1101 49 1.1226799235200957 1101 50 1.3790437267942934 1101 51 0.5873103300612001 2202 1 0.5267113247606758 2202 2 0.13418592422871 2202 3 0.4345368310588782 2202 4 0.6832018966067039 2202 5 -0.32214058399912915 2202 6 0.5451052061657364 2202 7 0.4279041042739138 2202 8 0.17282658810201923 2202 9 0.6976506481135303 2202 10 1.1366964946354514 2202 11 0.26195051932506885 2202 12 0.8037105563697224 2202 13 0.6393967816146713 2202 14 1.0050441001942403 2202 15 0.8186601860685201 2202 16 0.40672587671047467 2202 17 0.5616972212124196 2202 18 0.7875350080424894 2202 19 0.28461270459516186 2202 20 0.2666533938840274 2202 21 1.0199467593620781 2202 22 0.07948973208672783 2202 23 0.4586513532297971 2202 24 0.7556840365454036 2202 25 0.09377048085514872 2202 26 -0.08635279338524526 2202 27 0.48526366388625064 2202 28 0.7371216033964245 2202 29 0.5673037133493395 2202 30 0.5939432498203985 2202 31 0.03289722673071793 2202 32 0.2898071691686397 2202 33 -0.14291521676232577 2202 34 -0.08930105766347651 2202 35 -1.7725641325856998 2202 36 -1.072669389003707 2202 37 -0.9989382467864658 2202 38 -0.04944584457577861 2202 39 -0.4501170253410649 2202 40 -0.43307435082986245 2202 41 -0.30430079654117037 2202 42 -0.19637543666491553 2202 43 0.46403252511269955 2202 44 -0.29029638194499086 2202 45 -0.22574748909018894 2202 46 -0.6776475680571323 2202 47 -1.6739677593372875 2202 48 -0.9453252225753204 2202 49 -0.5518981355516317 2202 50 0.2102673554862733 2202 51 -0.9409948738769103 1102 1 -0.6685174970504235 1102 2 -0.7699036570760569 1102 3 0.5729659125259374 1102 4 0.5433122503879417 1102 5 -0.7431078024347002 1102 6 -0.2518762933474079 1102 7 0.10187359469910007 1102 8 0.2539690743077969 1102 9 0.5160537639849991 1102 10 0.02342158389218696 1102 11 0.560717153534739 1102 12 0.5705070510143244 1102 13 0.1479978851524848 1102 14 0.8894971998372085 1102 15 0.5601402250169158 1102 16 0.23340552617493415 1102 17 0.13375174896314945 1102 18 0.6315791495549874 1102 19 0.08256791394277485 1102 20 0.19362515629882893 1102 21 -0.3247465220400656 1102 22 0.01636087781863438 1102 23 0.14268251194449988 1102 24 0.6889849128842584 1102 25 -0.4567001455648452 1102 26 0.5397426301785302 1102 27 0.9640043325003376 1102 28 0.539620791971565 1102 29 0.17441179514096206 1102 30 0.18790624884668716 1102 31 0.4147537668151031 1102 32 0.0971749069161234 1102 33 -0.07329496359776755 1102 34 0.4546781698600526 1102 35 0.39118140457755857 1102 36 -0.003860560701205884 1102 37 0.3775628892083418 1102 38 0.353020887004881 1102 39 0.34264506488938196 1102 40 1.0894832603353493 1102 41 1.0147514592199443 1102 42 1.265000739901133 1102 43 0.4596508308575835 1102 44 0.565432277581138 1102 45 -0.021755278768864628 1102 46 0.9299473686517317 1102 47 -0.9522864870231089 1102 48 -0.20254690384763308 1102 49 0.08101325685343141 1102 50 0.6098129575635198 1102 51 0.6524812702321441 2203 1 -0.4377482662811971 2203 2 -0.2186216057940058 2203 3 0.11142745098747042 2203 4 -0.03361082653513847 2203 5 -0.3200308793577804 2203 6 -0.4057224471935612 2203 7 -0.11607512324961533 2203 8 -0.23321041287169209 2203 9 -0.00958726165602286 2203 10 -0.9381168776462729 2203 11 -1.0418469490293631 2203 12 -0.08333910283183599 2203 13 -0.3135405763859822 2203 14 -1.1105028001627915 2203 15 -0.31165464677795995 2203 16 -0.5101842174148103 2203 17 -1.1482995330881351 2203 18 -0.8171387991629615 2203 19 -0.30204747067261195 2203 20 0.0013174639911355257 2203 21 -0.9273106246041678 2203 22 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44 1.022391984540846 1103 45 0.4819077248941426 1103 46 1.096614035318396 1103 47 1.3087025239659056 1103 48 1.0703468690461335 1103 49 0.5810132568534314 1103 50 1.1885675363181036 1103 51 0.9345325522834216 2204 1 0.5997975212645912 2204 2 0.3674589802865782 2204 3 -0.07721723765721578 2204 4 0.10649906357475203 2204 5 -0.2128880222149192 2204 6 0.8287097872386724 2204 7 -0.09409710127159343 2204 8 0.21276028309900852 2204 9 1.1003028482340866 2204 10 0.25785381832442056 2204 11 -0.684704091886509 2204 12 0.4459649264721932 2204 13 -0.007679770525172103 2204 14 0.16055947089948575 2204 15 0.602264767141456 2204 16 0.23523702800643775 2204 17 0.46525358046498155 2204 18 0.6782824462582795 2204 19 -0.003512672137816253 2204 20 0.5342844969581648 2204 21 0.4536417563482189 2204 22 0.7416356030933642 2204 23 -0.15768378842179942 2204 24 0.14869187259121475 2204 25 -0.7552349440996409 2204 26 -0.3265577361218348 2204 27 0.36968198817799447 2204 28 0.630280132630908 2204 29 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0.9282432441073212 1213 1 0.03904218464718667 1213 2 -0.7433784581370659 1213 3 -0.4232763509577069 1213 4 -0.3709229397093168 1213 5 -0.34876651154168314 1213 6 -0.27309645249860637 1213 7 -0.17619890751133482 1213 8 0.05922990543069773 1213 9 -0.8446889415852894 1213 10 -0.0709639156657289 1213 11 -0.9987435007534984 1213 12 -0.5581401638397878 1213 13 -0.6952823977829752 1213 14 -0.9391942236817954 1213 15 -1.3616104381130612 1213 16 -0.9363557470346109 1213 17 -1.1792455985169568 1213 18 -0.5392873402770206 1213 19 -0.39554879693255884 1213 20 -0.47679924688419817 1213 21 -0.4646669464432449 1213 22 -0.5999079108639478 1213 23 -0.8440548885860082 1213 24 -0.35212914547118146 1213 25 -0.38331376183363375 1213 26 -1.0597268658426913 1213 27 -0.7271981431848928 1213 28 -0.8491944158091584 1213 29 -1.2871266663974978 1213 30 -0.8731017087129942 1213 31 -1.0019128998515612 1213 32 -1.9693591337558516 1213 33 -0.65950186014949 1213 34 -0.7696808044989183 1213 35 -1.3291987899405697 1213 36 -0.7156200655641598 1213 37 -0.9436130612779579 1213 38 -0.752195735453121 1213 39 -1.2338359253847173 1213 40 -1.408085263095245 1213 41 -0.8714112286668794 1213 42 -1.1664758295064743 1213 43 -0.8321263574713313 1213 44 -0.7440283767070994 1213 45 -0.09403644587761306 1213 46 -1.4235009072103395 1213 47 -1.2495897584643103 1213 48 -0.8992754626451571 1213 49 -0.5224350190086398 1213 50 -0.523255123780423 1213 51 -0.7321341143832427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 6.0 18 0.0 19 3.0 20 2.0 21 15.0 22 19.0 23 59.0 24 89.0 25 182.0 26 297.0 27 430.0 28 611.0 29 774.0 30 957.0 31 1252.0 32 1407.0 33 1739.0 34 2163.0 35 2581.0 36 3289.0 37 4845.0 38 8753.0 39 27101.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.53293254354169 28.54389532313677 13.77950667491822 25.143665458403326 2 10.537014777628508 29.940606660538784 40.2000989888991 19.322279572933606 3 17.98851082633672 32.48431285903668 30.338488731771985 19.18868758285462 4 12.613345117101193 27.93106495802033 33.40698188245692 26.048608042421563 5 11.171011931064958 39.609368095448524 36.90145824127264 12.318161732213875 6 30.451612903225804 41.56252761820592 15.42907644719399 12.556783031374282 7 19.674768007070263 32.783031374281926 26.21122403888643 21.33097657976138 8 19.056120194432168 43.50508174988953 22.303137428192667 15.13566062748564 9 20.358815731330093 19.787892178524082 21.09412284577994 38.759169244365886 10 13.258506407423774 30.296067167476803 37.14714980114891 19.298276623950507 11 26.756579993990066 23.436975235536387 26.89445495200891 22.91198981846464 12 19.047282368537342 25.424657534246574 34.09633230225364 21.431727794962438 13 29.935483870967744 21.426425099425543 28.853733981440566 19.78435704816615 14 17.095890410958905 24.033583738400356 28.003535130357932 30.86699072028281 15 23.738400353513036 29.60141405214317 25.52010605391074 21.140079540433053 16 20.557146392335703 29.335030225898823 31.737193763919823 18.370629617845655 17 16.807777286787452 33.910737958462214 32.91029606716748 16.371188687582855 18 23.42460184186803 25.966450426882083 34.09400243932618 16.51494529192371 19 16.86080424215643 34.409191338930626 28.959787892178525 19.770216526734423 20 17.39107379584622 27.02960671674768 34.483429076447194 21.095890410958905 21 14.33672116659302 32.56031816173221 29.007512152010605 24.09544851966416 22 16.39946973044631 34.974812196199736 28.279275298276623 20.346442775077332 23 14.243040212107822 28.15731330092797 30.71321254971277 26.886433937251436 24 19.29650905877154 33.14184710561202 31.690676093680953 15.870967741935486 25 17.422889969067608 31.25939019001326 30.281926646045072 21.03579319487406 26 17.728678745028724 29.048166151126825 34.77507733097658 18.448077772867872 27 18.812196199734863 32.0830755634114 32.30048608042422 16.80424215642952 28 14.764471939902785 28.136102518780383 31.72425983208131 25.375165709235525 29 19.356606274856386 27.93283252319929 34.53645603181617 18.17410517012815 30 15.411673206773429 30.77031852087531 34.82341711740376 18.9945911549475 31 18.244807777286788 26.492266902342028 31.611135660627486 23.651789659743702 32 18.937693327441448 28.249226690234202 33.6986301369863 19.114449845338047 33 16.62395050817499 29.155987627043743 33.42465753424658 20.79540433053469 34 15.162174105170129 28.89085285019885 38.48873177198409 17.45824127264693 35 14.147591692443658 33.31330092797172 32.65576668139637 19.883340698188245 36 14.983650022094563 28.406539991162173 38.729120636323465 17.880689350419797 37 14.195315952275738 31.49801148917366 32.85373398144056 21.452938577110032 38 14.821034025629695 28.13963764913831 36.65399911621741 20.385329209014582 39 15.575784357048168 26.762704374723818 35.89041095890411 21.771100309323906 40 15.510384445426425 27.56164383561644 36.434821034025624 20.493150684931507 41 15.745470614228898 25.596111356606276 35.57224922669023 23.086168802474592 42 17.617322138753867 28.48784798939461 34.95890410958904 18.935925762262485 43 13.451171011931065 27.718957136544407 36.41007512152011 22.419796730004418 44 12.749447635881573 32.50198851082634 34.313742819266466 20.43482103402563 45 13.224922669023421 28.134334953601414 35.85152452496686 22.78921785240831 46 16.07600530269554 27.75784357048166 33.05346884666372 23.11268228015908 47 14.522315510384445 26.92532037118869 34.00088378258948 24.551480335837383 48 12.337604949182502 26.91294741493593 31.080866106937695 29.668581528943882 49 17.33981440565621 26.621299160406544 33.46707909854176 22.571807335395494 50 12.220945647370748 27.574016791869198 34.88466637207247 25.320371188687584 51 11.68734754480857 27.572736592781137 30.773853713720083 29.96606214869021 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 490.0 1 282.5 2 75.0 3 124.0 4 173.0 5 157.0 6 141.0 7 152.0 8 163.0 9 187.5 10 212.0 11 308.0 12 404.0 13 545.5 14 687.0 15 757.0 16 827.0 17 679.5 18 532.0 19 421.5 20 311.0 21 343.0 22 375.0 23 357.0 24 339.0 25 321.0 26 369.0 27 435.0 28 557.0 29 679.0 30 1018.0 31 1357.0 32 1984.5 33 2612.0 34 3754.0 35 4896.0 36 5795.5 37 6695.0 38 7246.5 39 7798.0 40 8016.0 41 8234.0 42 7289.0 43 6344.0 44 5262.0 45 4180.0 46 3267.0 47 2354.0 48 1852.5 49 1351.0 50 1234.5 51 1118.0 52 1813.0 53 2508.0 54 1407.5 55 307.0 56 257.5 57 208.0 58 183.0 59 158.0 60 139.0 61 120.0 62 94.5 63 69.0 64 53.0 65 37.0 66 37.5 67 38.0 68 23.0 69 8.0 70 10.5 71 13.0 72 12.5 73 12.0 74 7.5 75 4.5 76 6.0 77 3.0 78 0.0 79 1.0 80 2.0 81 1.0 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03535130357931949 2 0.005302695536897923 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.003535130357931949 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0017675651789659744 17 0.0 18 0.003535130357931949 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0017675651789659744 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0017675651789659744 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 56575.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.25622254716781 #Duplication Level Percentage of deduplicated Percentage of total 1 92.01906831425141 65.56931210383337 2 5.372799546581729 7.656908003851 3 1.0817141695548447 2.3123659679467448 4 0.39453931508029294 1.1245352495587406 5 0.224720990815566 0.8006384466287012 6 0.11390502729299909 0.48698651844187 7 0.10546901145764795 0.5260726346579168 8 0.07455483541753921 0.4249996755583714 9 0.05932109918289 0.38042977006067624 >10 0.44857122541949607 6.468823629720317 >50 0.06859127047769206 3.700574367387272 >100 0.02939615557439394 4.812479060821935 >500 0.004899359262312182 2.290160510606022 >1k 0.002449679631156091 3.4457140609270485 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 1974 3.4891736632788337 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC 811 1.433495360141405 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 501 0.8855501546619531 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 462 0.8166151126822803 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 315 0.5567830313742819 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 256 0.45249668581528946 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG 244 0.4312859036676977 No Hit AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC 232 0.41007512152010606 No Hit TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT 228 0.40300486080424214 Illumina PCR Primer Index 11 (95% over 21bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 228 0.40300486080424214 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC 222 0.3923994697304463 No Hit AAAAAGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACATCTCCGA 203 0.3588157313300928 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 127 0.22448077772867875 No Hit AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA 124 0.2191780821917808 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTC 116 0.20503756076005303 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96 0.16968625718073355 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACA 96 0.16968625718073355 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAACTGTCTCTTATACACA 95 0.16791869200176757 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 95 0.16791869200176757 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 91 0.16084843128590368 No Hit CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG 91 0.16084843128590368 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 91 0.16084843128590368 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA 89 0.15731330092797172 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 84 0.14847547503314185 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 81 0.14317277949624393 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACATC 80 0.14140521431727796 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 78 0.137870083959346 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.13610251878038004 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGA 75 0.13256738842244808 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAACTGTCTCTTATACACAT 74 0.1307998232434821 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72 0.12726469288555015 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCCTGTCTCTTATACACATC 71 0.12549712770658417 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCACTGTCTCTTATACACATC 69 0.12196199734865222 No Hit AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA 69 0.12196199734865222 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68 0.12019443216968627 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACATCTC 68 0.12019443216968627 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCC 61 0.10782147591692443 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC 61 0.10782147591692443 No Hit GCGTTGATACCACTGCTTCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 60 0.10605391073795847 No Hit AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC 59 0.1042863455589925 No Hit GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC 58 0.10251878038002651 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0017675651789659744 5 0.0 0.0 0.0017675651789659744 6 0.0 0.0 0.0017675651789659744 7 0.0 0.0 0.0017675651789659744 8 0.0 0.0 0.008837825894829872 9 0.0 0.0 0.010605391073795847 10 0.0 0.0 0.015908086610693768 11 0.0 0.0 0.015908086610693768 12 0.0 0.0 0.015908086610693768 13 0.0 0.0 0.015908086610693768 14 0.0 0.0 0.019443216968625717 15 0.0 0.0 0.024745912505523642 16 0.0 0.0 0.031816173221387536 17 0.0 0.0 0.04242156429518339 18 0.0 0.0 0.04595669465311533 19 0.0 0.0 0.07247017233760494 20 0.0 0.0 0.0901458241272647 21 0.0 0.0 0.13256738842244808 22 0.0 0.0 0.2138753866548829 23 0.0 0.0 0.39946973044631023 24 0.0 0.0 0.9032258064516129 25 0.0 0.0 1.0198851082633673 26 0.0 0.0 1.654441007512152 27 0.0 0.0 1.942554131683606 28 0.0 0.0 2.760936809544852 29 0.0 0.0 3.787892178524083 30 0.0 0.0 4.288113124171454 31 0.0 0.0 7.20989836500221 32 0.0 0.0 8.684047724259832 33 0.0 0.0 9.767565178965974 34 0.0 0.0 11.79849756959788 35 0.0 0.0 13.1064958020327 36 0.0 0.0 15.040212107821477 37 0.0 0.0 16.72470172337605 38 0.0 0.0 18.449845338046842 39 0.0 0.0 20.32169686257181 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 200 0.0 41.625 45 ATGCCGT 200 0.0 41.625 44 CGTATGC 205 0.0 40.609756 41 TCGTATG 205 0.0 40.609756 40 TATCTCG 200 0.0 40.5 36 CTCGTAT 200 0.0 40.5 39 TATGCCG 210 0.0 39.642857 43 CGAGACG 210 0.0 39.642857 23 TCTCGTA 205 0.0 39.512196 38 ACGCTAT 200 0.0 39.375 30 CTTTAGG 40 3.3811375E-7 39.375 1 GACGCTA 200 0.0 39.375 29 AGACGAC 200 0.0 39.375 25 GACGACG 200 0.0 39.375 26 CGACGCT 200 0.0 39.375 28 CTATATC 195 0.0 39.23077 33 ACGAGAC 215 0.0 38.720932 22 GAGACGA 215 0.0 38.720932 24 CGCTATA 205 0.0 38.414635 31 ACGACGC 205 0.0 38.414635 27 >>END_MODULE