FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_71.3410000000605e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_71.3410000000605e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56575
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC19743.4891736632788337No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC8111.433495360141405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.8855501546619531No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4620.8166151126822803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.5567830313742819No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.45249668581528946No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG2440.4312859036676977No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC2320.41007512152010606No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT2280.40300486080424214Illumina PCR Primer Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.40300486080424214No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC2220.3923994697304463No Hit
AAAAAGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACATCTCCGA2030.3588157313300928No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.22448077772867875No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1240.2191780821917808No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTC1160.20503756076005303No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.16968625718073355No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACA960.16968625718073355No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAACTGTCTCTTATACACA950.16791869200176757No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.16791869200176757No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT910.16084843128590368No Hit
CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG910.16084843128590368No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.16084843128590368No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA890.15731330092797172No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.14847547503314185No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.14317277949624393No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACATC800.14140521431727796No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA780.137870083959346No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.13610251878038004No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGA750.13256738842244808No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAACTGTCTCTTATACACAT740.1307998232434821No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.12726469288555015No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCCTGTCTCTTATACACATC710.12549712770658417No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCACTGTCTCTTATACACATC690.12196199734865222No Hit
AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA690.12196199734865222No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.12019443216968627No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACATCTC680.12019443216968627No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCC610.10782147591692443No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC610.10782147591692443No Hit
GCGTTGATACCACTGCTTCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.10605391073795847No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC590.1042863455589925No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC580.10251878038002651No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2000.041.62545
ATGCCGT2000.041.62544
CGTATGC2050.040.60975641
TCGTATG2050.040.60975640
TATCTCG2000.040.536
CTCGTAT2000.040.539
TATGCCG2100.039.64285743
CGAGACG2100.039.64285723
TCTCGTA2050.039.51219638
ACGCTAT2000.039.37530
CTTTAGG403.3811375E-739.3751
GACGCTA2000.039.37529
AGACGAC2000.039.37525
GACGACG2000.039.37526
CGACGCT2000.039.37528
CTATATC1950.039.2307733
ACGAGAC2150.038.72093222
GAGACGA2150.038.72093224
CGCTATA2050.038.41463531
ACGACGC2050.038.41463527
Produced by FastQC (version 0.11.2)