FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_70.34100000006041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_70.34100000006041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276161
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC19210.6956087209997067No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA5820.2107466296834093No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC2890.1046490996194249No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1950.043.84456345
CGACGCT1950.042.6984928
ACGCTAT1950.042.6984930
ACGACGC1950.042.6984927
CGCTATA1950.042.6907631
GACGACG2050.041.7133626
ATCTCGT2050.041.70580737
TATGCCG2050.041.70580743
TATCTCG2050.041.70580736
TCTCGTA2050.041.70580738
ATGCCGT2050.041.70580744
CGAGACG2100.040.71281423
CCACGAG2100.040.71281420
ATATCTC2150.039.76635
GACGCTA2100.039.648629
ACGAGAC2250.038.9985922
AGACGAC2200.038.86926725
GAGACGA2200.038.86926724
CTCGTAT2200.038.8622339
CACGAGA2200.038.8622321
Produced by FastQC (version 0.11.2)