FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_7.34100000005bdd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_7.34100000005bdd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675481
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC56380.834664483530995No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC45830.6784794835087885No Hit
GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAGTACTCTGCGTTGA40740.6031257725976008No Hit
GCGTTGATACCACTGCTTGGCCAAGCAGTGGTATCAACGCAGAGTACTTTT34260.5071941327735348No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA33410.4946105071793285No Hit
GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAGTACTCTGCGTTGAT33290.4928339953307347No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA32630.48306318016346866No Hit
CCACTGCTTCGCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC30490.45138205219687894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC27390.40548882944153863No Hit
CCACTGCTTCGCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT26200.38787175360965No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCTGCGTTGATAC24570.363740800999584No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC23260.3443472133191015No Hit
GCGTTGATACCACTGCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT22900.33901767777332004No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCA22590.334428355497786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGCGAAGCAGTGGTATCAA22590.334428355497786No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGCGAAGCAGT22210.3288027346439056No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT21530.3187358341685406No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGCGAAGCAGTG20350.30126680099070147No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACC19940.29519705217467257No Hit
GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAGTACTCTGCGTTG19880.29430879625037565No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA19760.29253228440178186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGCGAAGCAGTGGTATCAAC19280.2854262370074066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG19090.28261342658046634No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATA18320.27121414221865603No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATC18000.2664767772890725No Hit
TACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT17280.2558177061975096No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCAGTGGTATC15850.23464760666843332No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC15680.23213088154959205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACGC15460.22887394316050338No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA15360.22739351662000856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC15270.22606113273356318No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG15180.22472874884711783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG14950.2213237678039797No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT14520.21495793367985183No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC14120.20903622751787246No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTG14100.2087401422097735No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTAT14050.20799992893952607No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA13680.20252235073969513No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAGTACTCTGCGTTGATACCA13620.2016340948153982No Hit
CCACTGCTTCGCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG13550.20059779623705182No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGCGAAGCAGTGGTAT13340.19748890050201262No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCAAGCAGTGGTATCA13270.19645260192366626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC13000.19245545026433014No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCA12980.19215936495623118No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG12900.19097502372383532No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATC12850.1902348104535879No Hit
GCGTTGATACCACTGCTTGCGAAAAAAAAAAAAAAAAGTACTCTGCGTTGA12800.18949459718334047No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCAAGCAGTGGTATCAACGCA12720.18831025595094458No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG12670.18757004268069716No Hit
GCGTTGATACCACTGCTTGCGAAAAAAAAAAAAAAAGTACTCTGCGTTGAT12490.18490527490780645No Hit
CTGCTTGGCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT12470.18460918959970748No Hit
ACTCTGCGTTGATACCACTGCTTGGCCAAGCAGTGGTATCAACGCAGAGTA12430.18401701898350953No Hit
GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAGTACTCTGCGTTGATA12420.18386897632946003No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTA12370.18312876305921263No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTT12320.18238854978896518No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGCGAAGCAG11970.17720705689723323No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT11950.17691097158913427No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCAAGCAGTGGTAT11860.1755785877026889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG11790.17454228912434253No Hit
CCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCG11780.17439424647029303No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGCGAAGCAGTGGTATC11760.17409816116219407No Hit
GATACCACTGCTTGGCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT11740.17380207585409507No Hit
ACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTAC11650.17246969196764972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA11500.17024905215690744No Hit
TGATACCACTGCTTGGCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT11220.16610385784352186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACG11140.164919516611126No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGCGAAGCAGTGGTATCA11100.16432734599492804No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCAC10990.16269887680038372No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT10790.15973802371939402No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCAGTGGTATCAA10550.1561850000222064No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTAT10350.1532241469412167No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG10280.1521878483628703No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCAAGCAGTGGTATCAA10250.15174372040072187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTGCGCGAAGCAGTGGTATCAAC10220.1512995924385734No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGAT10160.1504113365142765No Hit
CCACTGCTTCGCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT10030.1484867820116332No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACG10030.1484867820116332No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGCGAAGCAGTGG10000.14804265404948475No Hit
ACTCTGCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAGTACTCTGC9760.14448963035229712No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGT9680.14330528911990123No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG9630.1425650758496538No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC9630.1425650758496538No Hit
ACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTA9580.14182486257940638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTGCAAGCAGTGGTATCAACGCAG9450.1399003080767631No Hit
TACCACTGCTTCGCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT9370.13871596684436718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGCGAAGCAGTGGTATCA9360.13856792419031772No Hit
GATACCACTGCTTCGCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA9130.13516294314717955No Hit
ATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT9010.13338643129858574No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCAAGCAGTGGTATCAAC9010.13338643129858574No Hit
CCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTC9000.13323838864453624No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGT8990.13309034599048677No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTC8990.13309034599048677No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT8850.131017748833794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTGCGCGAAGCAGTGGTATCAACG8830.13072166352569503No Hit
ATACCACTGCTTGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT8800.13027753556354657No Hit
GCGTTGATACCACTGCTTGCGAAAAAAAAAAAAAAGTACTCTGCGTTGATA8770.1298334076013981No Hit
TACCACTGCTTCGCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG8670.12835298106090326No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC8670.12835298106090326No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAGT8570.1268725545204084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGCGAAGCAGTGGTATCAA8540.12642842655825995No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCA8500.12583625594206205No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG8390.1242077867475177No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTA8390.1242077867475177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC8340.12346757347727028No Hit
GATACCACTGCTTCGCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC8340.12346757347727028No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCA8260.1222832322448744No Hit
ACTCTGCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAGTACTCTGCG8210.12154301897462697No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGCGAAGCAGTGGT8200.12139497632057748No Hit
CCACTGCTTGGCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT8180.1210988910124785No Hit
GCGTTGATACCACTGCTTGCGAAAAAAAAAAAAAAAAAGTACTCTGCGTTG8130.1203586777422311No Hit
TACCACTGCTTGGCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT8100.11991454978008263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC8070.11947042181793417No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGCGAAGCAGTGGT8050.11917433650983521No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTG8000.1184341232395878No Hit
TGATACCACTGCTTCGCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACC7970.11798999527743934No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCAAGCAGTGGTATCA7940.11754586731529088No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGCGAAGCAGTGG7930.11739782466124139No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC7910.11710173935314244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA7860.116361526082895No Hit
ATACCACTGCTTGGCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTT7820.11576935546669706No Hit
ACGCAGAGTACTTTTTTTTTTTTTTGCGCGAAGCAGTGGTATCAACGCAGA7530.111476118499262No Hit
GTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA7450.11029177726686613No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT7290.10792309480207438No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCAAGCAGTGGTATCAACG7280.1077750521480249No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCAAGCAGTGGTA7250.10733092418587643No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCT7210.1067387535696785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACG7180.10629462560753004No Hit
ATACCACTGCTTGCGAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT7110.10525832702918364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACGCA7100.10511028437513417No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTA7030.10407398579678777No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTGCGCGAAGCAGTGGTATCAACG7030.10407398579678777No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACT6980.10333377252654036No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGA6960.10303768721844138No Hit
GATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT6910.10229747394819395No Hit
ATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC6900.10214943129414446No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAGTAC6820.10096509006174859No Hit
ATACCACTGCTTGCGAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC6780.10037291944555064No Hit
GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAGTACTCTGCGTT6770.10022487679150116No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATCT207.032358E-444.9985234
CGATAAC207.032358E-444.9985228
AACCTAT207.032358E-444.9985232
TAACCTA207.032358E-444.9985231
AGACGAT253.8898474E-544.9985225
GACGATA207.032358E-444.9985226
ACCTATC207.032358E-444.9985233
ACGATAA207.032358E-444.9985227
GTATGCC1300.041.53709842
TGCCGTC1300.041.53709845
TATGCCG1300.041.53709843
ATGCCGT1300.041.53709844
CGTATGC1300.041.53709841
TCGTATG1300.041.53709840
CTCGTAT1250.041.3986439
TATCTCG1200.041.24864236
CTATCTC1150.041.085635
TTGCTAT1000.040.49866532
GCTATCT950.040.2618334
ATCTCGT1300.039.80638537
Produced by FastQC (version 0.11.2)