FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_69.34100000006034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_69.34100000006034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221726
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC48432.184227379738957No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA4100.18491291052921174No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT2450.1104967392186753Illumina PCR Primer Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4150.040.66244545
CGCTATA4400.038.3520831
ACGCTAT4400.038.3520830
ACGACGC4400.038.3520827
CTCGTAT4500.037.9998139
TATGCCG4500.037.9998143
GACGCTA4450.037.92115829
GACGACG4450.037.92115826
ATCTCGT4550.037.5822337
AGACGAC4550.037.5822325
GAGACGA4550.037.5822324
TATCTCG4550.037.5822336
CGACGCT4500.037.49981328
TCTCGTA4600.037.1737338
ATGCCGT4550.037.08772744
CGAGACG4650.036.7740123
CCACGAG4750.036.47350320
TCGTATG4700.036.38279740
GCCCACG4800.036.0935718
CTATATC4800.036.0935733