FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_68.34100000006027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_68.34100000006027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360624
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC14650.4062402946004703No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA13930.3862749012822219No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC8300.23015661741869647No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT7810.2165690580771108No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG5070.14058964461599893No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA4540.12589289675673276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG1950.042.69072723
TATCTCG2100.041.7841736
ACGAGAC2100.041.7841722
GAGACGA2000.041.62346324
CGCTATA2000.041.62346331
GACGCTA1950.041.53692229
GCCCACG2150.040.81244318
TGCCGTC2150.040.81244345
TCTCGTA2100.040.7127838
CCCACGA2200.039.8848919
CCACGAG2200.039.8848920
CTCGTAT2150.039.76597239
ACGCTAT2100.039.6413930
CGACGCT2050.039.51073528
GACGACG2050.039.51073526
CCGAGCC2300.039.1344214
CGAGCCC2300.039.1344215
ATCTCGT2200.038.862237
CGTATGC2200.038.862241
TCGTATG2200.038.862240
Produced by FastQC (version 0.11.2)