FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_67.3410000000601a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_67.3410000000601a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320013
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC15790.493417454915894No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA10170.31779958939168096No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT5760.17999268779705824No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC3600.1124954298731614No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT2600.038.94095628
CCACGAG2600.038.94095620
GACGACG2550.038.82217826
CGCTATA2500.038.69865831
ACGACGC2600.038.07560327
ACGCTAT2550.037.93985730
ACGAGAC2700.037.498722
CGAGACG2750.036.81690223
GACGCTA2700.036.66539429
TATGCCG2650.036.50816743
CCCACGA2800.036.1594619
GAGACGA2800.036.1594624
GTAGTGG250.002105200435.9987536
AGACGAC2750.035.9987525
TCGTATG2700.035.8320940
ATGCCGT2700.035.8320944
TCTCCGA2900.035.69399311
CCGAGCC2850.035.53063214
CACGAGA2850.035.5250821
CTCGTAT2750.035.18059539
Produced by FastQC (version 0.11.2)