FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_65.34100000005fe6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_65.34100000005fe6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15773
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7074.482343244785393No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC4973.15095416217587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2321.4708679388829011No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.8622329296899764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.7924935015532872No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.4754961009319723No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT490.3106574526088886Illumina PCR Primer Index 11 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.18385849236036264No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.14581880428580485No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.12679896024852597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATC351.1132215E-745.033
ATCTCGT406.1099854E-945.037
ATCTCCG406.1099854E-945.010
TATATCT351.1132215E-745.034
TGCCGTC406.1099854E-945.045
CCACGAG406.1099854E-945.020
TATGCCG406.1099854E-945.043
CATCTCC406.1099854E-945.09
GTATCAA700.045.01
GCTATAT351.1132215E-745.032
TCTCGTA406.1099854E-945.038
ACGAGAC406.1099854E-945.022
TATCTCG351.1132215E-745.036
AGACGAC351.1132215E-745.025
ATGCCGT406.1099854E-945.044
CGTATGC406.1099854E-945.041
GACGACG351.1132215E-745.026
CGAGCCC406.1099854E-945.015
CCGAGCC406.1099854E-945.014
CGACGCT351.1132215E-745.028