##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_65.34100000005fe6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15773 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.27864071514614 38.0 38.0 38.0 32.0 38.0 2 36.329043301844926 38.0 38.0 38.0 32.0 38.0 3 36.30482470043746 38.0 38.0 38.0 32.0 38.0 4 36.37786090154061 38.0 38.0 38.0 32.0 38.0 5 36.467697964876685 38.0 38.0 38.0 32.0 38.0 6 38.35998224814556 40.0 38.0 40.0 38.0 40.0 7 38.242376212515055 40.0 38.0 40.0 38.0 40.0 8 38.18423888924111 40.0 38.0 40.0 38.0 40.0 9 38.245799784441765 40.0 38.0 40.0 38.0 40.0 10 38.15298294553985 40.0 38.0 40.0 38.0 40.0 11 38.106574526088885 40.0 38.0 40.0 38.0 40.0 12 38.039941672478285 40.0 38.0 40.0 32.0 40.0 13 38.03442591770747 40.0 38.0 40.0 32.0 40.0 14 37.95999492804159 40.0 38.0 40.0 32.0 40.0 15 38.026437583211816 40.0 38.0 40.0 32.0 40.0 16 38.02884676345654 40.0 38.0 40.0 32.0 40.0 17 38.02948075825778 40.0 38.0 40.0 32.0 40.0 18 38.021429024282 40.0 38.0 40.0 32.0 40.0 19 38.129081341533 40.0 38.0 40.0 38.0 40.0 20 38.03436251822735 40.0 38.0 40.0 32.0 40.0 21 38.09547961706714 40.0 38.0 40.0 38.0 40.0 22 38.08394091168452 40.0 38.0 40.0 32.0 40.0 23 38.0470424142522 40.0 38.0 40.0 32.0 40.0 24 38.03816648703481 40.0 38.0 40.0 32.0 40.0 25 38.05281176694351 40.0 38.0 40.0 32.0 40.0 26 37.969695048500604 40.0 38.0 40.0 32.0 40.0 27 37.92816838901921 40.0 38.0 40.0 32.0 40.0 28 37.88993850250428 40.0 38.0 40.0 32.0 40.0 29 37.856843973879414 40.0 38.0 40.0 32.0 40.0 30 37.946110441894376 40.0 38.0 40.0 32.0 40.0 31 37.86540290369619 40.0 38.0 40.0 32.0 40.0 32 37.832625372471945 40.0 38.0 40.0 32.0 40.0 33 37.8001014391682 40.0 38.0 40.0 32.0 40.0 34 37.82710961770113 40.0 38.0 40.0 32.0 40.0 35 37.7875483421036 40.0 38.0 40.0 32.0 40.0 36 37.74253471121537 40.0 38.0 40.0 32.0 40.0 37 37.75299562543587 40.0 38.0 40.0 32.0 40.0 38 37.67406327268117 40.0 38.0 40.0 32.0 40.0 39 37.54999049007798 40.0 38.0 40.0 32.0 40.0 40 37.52456729854815 40.0 38.0 40.0 32.0 40.0 41 37.51233119888417 40.0 38.0 40.0 32.0 40.0 42 37.40455208267292 40.0 38.0 40.0 32.0 40.0 43 37.38661002979776 40.0 38.0 40.0 32.0 40.0 44 37.19229062321689 40.0 38.0 40.0 32.0 40.0 45 37.1160210486274 40.0 38.0 40.0 32.0 40.0 46 36.9506118049832 40.0 38.0 40.0 32.0 40.0 47 36.86787548342104 40.0 38.0 40.0 32.0 40.0 48 36.747416471184934 40.0 38.0 40.0 32.0 40.0 49 36.66569454130476 40.0 38.0 40.0 27.0 40.0 50 36.644772712863755 40.0 38.0 40.0 27.0 40.0 51 36.22855512584797 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 -1.4619262857759523 2201 2 -0.055758330519381616 2201 3 0.7736856219079371 2201 4 -1.1660226801680125 2201 5 -1.7880339984874212 2201 6 -1.3747233514388455 2201 7 -0.7911764968250026 2201 8 -0.41729808544038605 2201 9 0.592413898280995 2201 10 0.7015885294467878 2201 11 0.4398622029327228 2201 12 0.8098164474730609 2201 13 -0.6180296731387003 2201 14 0.6381680162447481 2201 15 0.9021745459570738 2201 16 -0.0026573886957308446 2201 17 0.8315560095283345 2201 18 1.0082658483951832 2201 19 0.927238031999515 2201 20 -0.19541899537136231 2201 21 -0.25018377610820863 2201 22 0.8372828875836333 2201 23 0.4392872209898826 2201 24 0.9231132609874706 2201 25 0.7812013808648786 2201 26 1.2349370172646488 2201 27 1.589766425838043 2201 28 1.2211102078032354 2201 29 0.9569165351915174 2201 30 -0.801658425542648 2201 31 1.1012749111795728 2201 32 0.2889517637755219 2201 33 0.718396381042929 2201 34 1.5259867739208346 2201 35 -0.13224562905590176 2201 36 -0.9848542779662637 2201 37 -0.10353904527044477 2201 38 1.6549680850188295 2201 39 1.637400325712349 2201 40 0.15276319233683466 2201 41 1.2052452742279982 2201 42 0.2571963609571455 2201 43 1.1524098947170955 2201 44 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0.27600957605720566 1215 21 0.22838765246321913 1215 22 0.23728288758363192 1215 23 1.2250015067041673 1215 24 0.008827546701752453 1215 25 0.3740585237220273 1215 26 0.7135084458360765 1215 27 0.16119499726661246 1215 28 1.121110207803234 1215 29 0.44977367804866475 1215 30 0.3483415744573506 1215 31 0.6155606254652852 1215 32 0.70323747806124 1215 33 -0.3744607618142197 1215 34 -0.24544179750773765 1215 35 0.8820400852298107 1215 36 0.272288579176589 1215 37 0.3964609547295552 1215 38 0.6763966564474018 1215 39 0.6445431828552017 1215 40 0.9384774780511194 1215 41 1.740959559942283 1215 42 1.3929106466714316 1215 43 1.473838466145665 1215 44 1.3149064139040973 1215 45 0.2932692624105897 1215 46 0.3943588504184774 1215 47 1.0943066011567808 1215 48 1.3568318628057554 1215 49 1.6937442133265463 1215 50 1.7194927233827926 1215 51 1.8881106996388866 1212 1 -1.3234647473144108 1212 2 -0.3557583305193788 1212 3 0.9313779296002451 1212 4 -0.9429457570910955 1212 5 -1.157264767718189 1212 6 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0.20087753694915733 1213 28 -0.1751860884930636 1213 29 -0.5727130944381074 1213 30 0.6909341670499458 1213 31 0.669793429698089 1213 32 0.3130258378496009 1213 33 1.0832111958577428 1213 34 1.0667275146615722 1213 35 1.0103469635366906 1213 36 0.9058864627744754 1213 37 1.2575720658406695 1213 38 0.5771903072410538 1213 39 0.3151781034901262 1213 40 0.7731335627072085 1213 41 1.0478378668205934 1213 42 -0.355766602005815 1213 43 0.14315063545783602 1213 44 -1.9231888241911363 1213 45 -1.4606989915576705 1213 46 -1.6215141654545349 1213 47 0.16838067523085698 1213 48 0.36344561941950815 1213 49 0.1527389223212552 1213 50 -0.15484590095583428 1213 51 -0.8433707818425944 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 2.0 20 1.0 21 0.0 22 9.0 23 13.0 24 28.0 25 52.0 26 73.0 27 123.0 28 151.0 29 188.0 30 230.0 31 317.0 32 344.0 33 444.0 34 600.0 35 698.0 36 919.0 37 1294.0 38 2417.0 39 7869.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.8829200228325 23.33354474535422 13.33798439779286 25.445550834020423 2 14.145320821709358 25.006340349987322 39.77935582044129 21.068983007862034 3 17.973752615228555 28.58682558803018 30.019653838838522 23.419767957902746 4 11.824003043175047 24.890635896785646 34.47663729157421 28.8087237684651 5 12.217079819945477 38.15380713878146 35.205731313003234 14.42338172826983 6 28.168389019210043 43.29550497685919 15.051036581500032 13.485069422430737 7 23.730425410511632 36.49274075952577 19.210042477651683 20.56679135231091 8 22.14543840740506 40.82292525201293 18.404869080073542 18.626767260508466 9 22.969631649020478 21.295885373739935 20.357573067900844 35.37690990933874 10 14.734039180878716 36.04260445064351 28.396627147657387 20.82672922082039 11 27.990870474862106 28.884803144614214 24.7828567805744 18.34146959994928 12 22.012299499144106 29.24618018132251 27.052558169023015 21.688962150510367 13 24.288340835605148 27.287136245482785 28.631205224117164 19.7933176947949 14 19.20370252963926 27.033538324985734 27.014518480948457 26.748240664426554 15 20.053255563304383 33.56368477778482 24.86527610473594 21.517783554174855 16 22.012299499144106 32.91067013250492 25.784568566537754 19.292461801813225 17 17.682115006656947 35.744626894059465 27.014518480948457 19.55873961833513 18 20.64417955871164 29.717220390565558 31.346690337306622 18.291909713416178 19 18.056171939390097 30.59658910796931 30.767767704304827 20.579471248335764 20 18.620427312496037 29.588537373993535 30.957966144677613 20.833069168832814 21 17.580675838458124 29.740696126291766 31.268623597286506 21.410004437963607 22 16.95302098522792 33.677803842008494 31.630000633994804 17.739174538768783 23 17.663095162619666 30.5205097318202 31.338363025423195 20.47803208013694 24 22.132758511380207 28.574145692005327 31.883598554491854 17.409497242122615 25 18.005452355290686 33.462245609586 31.20522411716224 17.327077917961073 26 20.915488492994356 29.601217270018388 32.07379699486464 17.409497242122615 27 16.914981297153364 34.21035947505231 31.325683129398342 17.548976098395993 28 17.682115006656947 29.677296646167502 31.059405312876436 21.58118303429912 29 20.940848285044062 29.949914410701833 31.262283649274075 17.84695365498003 30 17.607151914785696 32.6211006847578 31.54958153690084 18.222165863555666 31 17.884993343054585 29.677296646167502 31.243263805236797 21.194446205541116 32 20.17371457554048 30.33031129144741 31.002345780764596 18.493628352247512 33 17.637735370569963 30.564889367907185 31.725099854181195 20.07227540734166 34 17.67577505864452 30.44443035567108 34.50833703163634 17.371457554048057 35 17.77721422684334 33.01844924871616 31.23692385722437 17.967412667216127 36 17.20027895771255 30.102073163000064 35.23109110505294 17.46655677423445 37 17.75819438280606 32.600012679896025 31.376402713497747 18.265390223800164 38 17.250998541811956 29.759715970329044 34.72389526405883 18.265390223800164 39 17.834273758955177 29.277879921384642 31.71241995815634 21.175426361503835 40 18.398529132061118 28.681924808216575 34.666835731946996 18.25271032777531 41 18.227350535725606 27.889431306663287 31.376402713497747 22.50681544411336 42 21.175426361503835 29.588537373993535 31.10378494896342 18.132251315539214 43 17.669435110632094 29.208140493247953 34.65415583592215 18.468268560197806 44 16.921321245165792 33.15158815697711 31.160844481075255 18.766246116781844 45 17.663095162619666 28.948202624738478 34.73023521207126 18.658467000570596 46 20.833069168832814 28.796043872440247 30.79312749635453 19.577759462372406 47 18.449248716160525 28.700944652253852 30.38103087554682 22.4687757560388 48 16.826222024979394 29.861155138527863 30.495149939770492 22.817472896722247 49 21.04862740125531 28.688264756229 30.932606352627907 19.330501489887784 50 17.54263615038357 28.948202624738478 33.791922906232166 19.717238318645787 51 16.97838077727763 29.740696126291766 30.393710771571676 22.887212324858936 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 727.0 1 375.5 2 24.0 3 36.0 4 48.0 5 39.5 6 31.0 7 24.5 8 18.0 9 34.0 10 50.0 11 87.5 12 125.0 13 192.5 14 260.0 15 323.0 16 386.0 17 332.5 18 279.0 19 234.0 20 189.0 21 182.0 22 175.0 23 178.0 24 181.0 25 186.5 26 228.5 27 265.0 28 304.0 29 343.0 30 448.0 31 553.0 32 628.0 33 703.0 34 778.0 35 853.0 36 896.5 37 940.0 38 948.0 39 956.0 40 908.5 41 861.0 42 843.5 43 826.0 44 830.5 45 835.0 46 815.5 47 796.0 48 783.0 49 770.0 50 817.0 51 864.0 52 1052.0 53 1240.0 54 918.0 55 596.0 56 555.0 57 514.0 58 466.0 59 418.0 60 366.0 61 314.0 62 245.5 63 177.0 64 142.0 65 107.0 66 84.0 67 61.0 68 52.5 69 44.0 70 33.0 71 22.0 72 19.0 73 16.0 74 10.0 75 6.0 76 8.0 77 4.5 78 1.0 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03803968807455779 2 0.0063399480124262985 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0063399480124262985 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0063399480124262985 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 15773.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.31617047197085 #Duplication Level Percentage of deduplicated Percentage of total 1 87.0997803025731 62.11622780129511 2 10.571468984007979 15.078333684053307 3 1.4465981789911107 3.0949752701211795 4 0.3518817207062703 1.0037942711943522 5 0.18595833128672912 0.6630918027363801 6 0.0 0.0 7 0.04923489702776436 0.24578710167213821 8 0.03689810856030274 0.210514544014387 9 0.07377468917341483 0.47351954786471223 >10 0.11064350583630232 1.5781235615374718 >50 0.012293546972840238 0.6575465187151918 >100 0.04917418789136095 8.67961404704053 >500 0.012293546972840238 6.198471849755207 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 707 4.482343244785393 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 497 3.15095416217587 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 1.4708679388829011 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 136 0.8622329296899764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 125 0.7924935015532872 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 75 0.4754961009319723 No Hit TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT 49 0.3106574526088886 Illumina PCR Primer Index 11 (95% over 21bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29 0.18385849236036264 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23 0.14581880428580485 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.12679896024852597 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0063399480124262985 22 0.0 0.0 0.0063399480124262985 23 0.0 0.0 0.0063399480124262985 24 0.0 0.0 0.038039688074557786 25 0.0 0.0 0.06339948012426298 26 0.0 0.0 0.09509922018639447 27 0.0 0.0 0.10777911621124707 28 0.0 0.0 0.13947885627337855 29 0.0 0.0 0.22189818043492043 30 0.0 0.0 0.3360172446585938 31 0.0 0.0 0.45647625689469346 32 0.0 0.0 0.6403347492550561 33 0.0 0.0 0.9066125657769606 34 0.0 0.0 1.2299499144107018 35 0.0 0.0 1.6103467951562798 36 0.0 0.0 1.933684143790021 37 0.0 0.0 2.3648006086350093 38 0.0 0.0 2.871996449629113 39 0.0 0.0 3.4108920306853485 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATATC 35 1.1132215E-7 45.0 33 ATCTCGT 40 6.1099854E-9 45.0 37 ATCTCCG 40 6.1099854E-9 45.0 10 TATATCT 35 1.1132215E-7 45.0 34 TGCCGTC 40 6.1099854E-9 45.0 45 CCACGAG 40 6.1099854E-9 45.0 20 TATGCCG 40 6.1099854E-9 45.0 43 CATCTCC 40 6.1099854E-9 45.0 9 GTATCAA 70 0.0 45.0 1 GCTATAT 35 1.1132215E-7 45.0 32 TCTCGTA 40 6.1099854E-9 45.0 38 ACGAGAC 40 6.1099854E-9 45.0 22 TATCTCG 35 1.1132215E-7 45.0 36 AGACGAC 35 1.1132215E-7 45.0 25 ATGCCGT 40 6.1099854E-9 45.0 44 CGTATGC 40 6.1099854E-9 45.0 41 GACGACG 35 1.1132215E-7 45.0 26 CGAGCCC 40 6.1099854E-9 45.0 15 CCGAGCC 40 6.1099854E-9 45.0 14 CGACGCT 35 1.1132215E-7 45.0 28 >>END_MODULE