FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_63.34100000005fcc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_63.34100000005fcc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222049
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC28441.2807983823390334No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA8070.3634332962544303No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC3560.16032497331670037No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT3030.13645636773865227No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT2290.10313039013911343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC3300.042.27082433
AGGCGAA3250.042.22886731
GGCGAAT3250.042.22886732
CGAATCT3350.040.96830734
TGCCGTC3850.040.9072545
CGAGACC3450.040.43296423
CGTATGC3900.040.38279741
TATGCCG3900.040.38279743
ACGAGAC3900.040.38279722
AAGGCGA3400.040.3749230
CTCGTAT3850.040.32285739
TCGTATG3950.039.87162440
ATGCCGT3950.039.87162444
CAAGGCG3450.039.7897829
GCCCACG4000.039.37322618
CCCACGA4000.039.37322619
CCACGAG4050.038.8871420
TCTCGTA4000.038.8107538
GTATGCC4100.038.41290342
ATCTCGT4050.038.33160837