H23NVBCXX l02n01 36h_62.34100000005fbf.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 36h_62.34100000005fbf.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1515
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	33	2.178217821782178	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC	16	1.056105610561056	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	14	0.9240924092409241	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	14	0.9240924092409241	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	10	0.6600660066006601	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC	7	0.46204620462046203	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	7	0.46204620462046203	TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	7	0.46204620462046203	RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	5	0.33003300330033003	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC	5	0.33003300330033003	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC	3	0.19801980198019803	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	3	0.19801980198019803	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATACCGTC	2	0.132013201320132	No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT	2	0.132013201320132	Illumina PCR Primer Index 11 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT	2	0.132013201320132	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAAACCTATCTCGTATGCCGTC	2	0.132013201320132	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG	2	0.132013201320132	No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT	2	0.132013201320132	No Hit
ACTTTACATCAGCCACTATAATTATCGCAATTCCTACCGGTGTCAAAGTAT	2	0.132013201320132	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC	2	0.132013201320132	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCCAC	20	5.01263E-4	45.0	17
GTATGCC	20	5.01263E-4	45.0	42
ATCTCGT	20	5.01263E-4	45.0	37
ATCTCCG	20	5.01263E-4	45.0	10
GCCCACG	20	5.01263E-4	45.0	18
CCACGAG	20	5.01263E-4	45.0	20
TATGCCG	20	5.01263E-4	45.0	43
GAGCCCA	20	5.01263E-4	45.0	16
CACGAGA	20	5.01263E-4	45.0	21
CCCACGA	20	5.01263E-4	45.0	19
ACATCTC	20	5.01263E-4	45.0	8
TCTCGTA	20	5.01263E-4	45.0	38
ACGAGAC	20	5.01263E-4	45.0	22
CGTATGC	20	5.01263E-4	45.0	41
CGAGCCC	20	5.01263E-4	45.0	15
CCGAGCC	20	5.01263E-4	45.0	14
TCGTATG	20	5.01263E-4	45.0	40
TCTCCGA	20	5.01263E-4	45.0	11
TCCGAGC	20	5.01263E-4	45.0	13
CTCGTAT	20	5.01263E-4	45.0	39

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content