FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_61.34100000005fa3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_61.34100000005fa3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306554
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC33201.0830065828532658No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA8460.27597095454634424No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT4590.14972892214748462No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC4080.13309237524220854No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA3240.10569100386881268No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT3650.039.45091237
TATGCCG3650.039.45091243
TGCCGTC3650.039.45091245
CGAGACC3450.039.129323
CTCGTAT3750.038.99886739
TCTCGTA3700.038.9177938
ACGAGAC3700.038.9177922
GCGAATC3500.038.5703133
CACGAGA3700.038.309721
CCCACGA3700.038.309719
CCGAGCC3700.038.309714
GCCCACG3650.038.2180718
CGAGCCC3650.038.2180715
CGAATCT3550.038.02706534
GGCGAAT3550.038.02706532
AGGCGAA3600.037.49891331
TCGTATG3900.037.49891340
AAGGCGA3650.036.9852330
ATGCCGT3900.036.9220144
TCCGAGC3850.036.81711613