FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_60.34100000005f96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_60.34100000005f96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62079
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC31265.035519257720002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11141.7944876689379663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8191.3192867153143575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.853750865832246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.615344963675317No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.5412458319238389No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT3190.5138613701895971No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2330.37532821082813833No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.29961822838641083No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.2174648431836853No Hit
ATGCAATTCCATTAGATGATGACTCCTTTCATTTCCATTCGATGATGATTC1320.21263229111293677No Hit
GAATAATCATTGAACGGAATCGAATGGAATCATCATCGAATGGAAACGAAT1300.2094105897324377No Hit
GAATTGAATGGAATCATCGAATGGTCTCGATTGGAATCATTATCAAATGGA1240.1997454855909406No Hit
ATTCGAGTCCATTCGATGATTCTCTTCGATTCCATTCGATAATTCTGCTTG1230.19813463490069108No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.19813463490069108No Hit
TCATAGAATGGAATCGAATGGATTCATTGAATGGAATCAGATGGAATCATC1090.17558272523719776No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.17397187454694824No Hit
GTGCAATACTCTGTCAATGGGATAGATTTTAGGAAAATTCCAGCTGAACCA1050.16913932247619967No Hit
GAATGGAATGGAACAGTCAATGAACTCGAATGGAATCATCATTGAATGGAA1050.16913932247619967No Hit
GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.16752847178595015No Hit
GAATGGAACAGTCAATGAACTCGAATGGAATCATCATTGAATGGAATCGAA1020.1643067704054511No Hit
ATTCAATTCCGTTCAATGATTCCATTTGATTCCATTTGATGTTGATTCCGT1010.1626959197152016No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.1626959197152016No Hit
ACTAAGTACACTGTCCAAACACTTTGGAAGTAGTTTTGTTTTCATTTTAGT1010.1626959197152016No Hit
GAATTAACCTCAAATGGAATCGAATGGAATTAACATTGAATGGACTCGAAT960.15464166626395398No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.15464166626395398No Hit
ATGTTAATTCCATTCGATTCCATTTGAGGTTAATTCCATTTGAGTCCATTC950.1530308155737045No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.15141996488345497No Hit
GTCTCGATTGGAATCATTATCAAATGGAATCGAATGGAATCACCGAATAGA910.1465874128127064No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA890.14336571143220736No Hit
CTAAGTACACTGTCCAAACACTTTGGAAGTAGTTTTGTTTTCATTTTAGTT880.1417548607419578No Hit
TTCCATGCAATTCCATTAGATGATGACTCCTTTCATTTCCATTCGATGATG860.1385331593614588No Hit
CGATTGGAATCATTATCAAATGGAATCGAATGGAATCACCGAATAGACTCG860.1385331593614588No Hit
ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.1320897566004607No Hit
GTATAGAATCGAATGGAATCACTGAATGTACTCGAATGGAATAATCATTGA820.1320897566004607No Hit
ATTCCATTCCATTCCATTTGAAGATGATTCCATTAGAGACCATTCGATGAA810.1304789059102112No Hit
GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.1304789059102112No Hit
ATTCAATTCCATTCAATAATGATCCCTTTCGAGTCCATTCAATGATTCCAT810.1304789059102112No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.12725720452971212No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA780.12564635383946263No Hit
AATATGGACTTTTCCTTCTTCAGAACGAACTTCTTCTGCTGAGTTTCCATG760.12242465245896358No Hit
ATCTAATGGAATTGCATGGAATCATCATCAAATGGAATCGAACGGAATCAA760.12242465245896358No Hit
ATTCTATACGATGATGATTGCATTCAAGTCCGTGGATTATTCCATTCCATT750.12081380176871405No Hit
TTATTGAATGGAATTGAATGGAATCATCGAATGGTCTCGATTGGAATCATT740.11920295107846453No Hit
TGATGATTCCATGCAATTCCATTAGATGATGACTCCTTTCATTTCCATTCG730.11759210038821502No Hit
GAATGGAATAATCCACGGACTTGAATGCAATCATCATCGTATAGAATCGAA720.1159812496979655No Hit
GATGTGTACATACAGAAAGGGTGGTGAGAAAGACAACTCTAGGTTTAGGGA720.1159812496979655No Hit
CAGTTATACAGACTAAATCCACATCTAAATGTGTACAAACAACATGGAAAA720.1159812496979655No Hit
CCATTAGATGATTCCATTCGAGTCCATTCGATGATTCTCTTCGATTCCATT720.1159812496979655No Hit
AGATGATACCATTCGAGACCATTTGATGATTGCATTCAATTCACTCGATGA710.11437039900771598No Hit
TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.11437039900771598No Hit
GAATTGAATGGAATTATCGAATGGTCTCGATTGGAATCATTATCAAAAGGA710.11437039900771598No Hit
CACTGAATGTACTCGAATGGAATAATCATTGAACGGAATCGAATGGAATCA700.11275954831746644No Hit
ATGTATGGCAGCTTTTTGAAGAATGTTTTAGGTCTTTCTAAATATGCAGCT700.11275954831746644No Hit
ATTCTATTCGATTGCATTCGATAATTCCATTCGATTCCATTTGAGGATAAT700.11275954831746644No Hit
GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.11275954831746644No Hit
GTATTGAATGGAATTGTCATCAAATGGAATTCAAAGGAATCATCATCAAAT700.11275954831746644No Hit
GTCAATGAACTCGAATGGAATCATCATTGAATGGAATCGAATGGAATCATC690.11114869762721694No Hit
ATCTAATGGAATGGAATGGAATAATCCACGGACTTGAATGCAATCATCATC690.11114869762721694No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.11114869762721694No Hit
GACTTGAATGCAATCATCATCGTATAGAATCGAATGGAATCACTGAATGTA690.11114869762721694No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.10953784693696741No Hit
CCATTAGATGATGACTCCTTTCATTTCCATTCGATGATGATTCCATTCGTT670.1079269962467179No Hit
ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA650.10470529486621885No Hit
GAATTGAATGGAATCGTCATCGAATGAATTGAATGCATTCATCGAATGGTC650.10470529486621885No Hit
ATTACAAACATGCCAATCCTTAGCCTGAATAGCTGCATATTTAGAAAGACC650.10470529486621885No Hit
AATCGAGACCATTCGATGATTCCATTCAATTCCATTCAATAATGATCCCTT640.10309444417596933No Hit
AGTCCATTCAATGTTAATTCCATTCGATTCCATTTGAGGTTAATTCCATTT640.10309444417596933No Hit
GAATCACTGAATGTACTCGAATGGAATAATCATTGAACGGAATCGAATGGA640.10309444417596933No Hit
TAAGTACACTGTCCAAACACTTTGGAAGTAGTTTTGTTTTCATTTTAGTTC640.10309444417596933No Hit
GAATGGAATAATCCATGGACTCGAATGCAATCATCTTCGAATGGAATCGAA640.10309444417596933No Hit
TGAAAGGAGTCATCATCTAATGGAATTGCATGGAATCATCATCAAATGGAA630.10148359348571981No Hit
GTATCATCTTCTAATGGAAAGGAATGGAATCATCGCATAGAATCGAATGGA630.10148359348571981No Hit
GAATCATCATCGAATGGAAACGAATGGAATCATCATCGAATGGAAATGAAA630.10148359348571981No Hit
ATTCGAGTCCATTCAATGTTAATTCCATTCGATTCCATTTGAGGTTAATTC630.10148359348571981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3450.039.78118541
TCGTATG3450.039.78118540
CTCGTAT3450.039.78118539
TGCCGTC3400.039.7364845
GTATGCC3400.039.7044642
ATCTCGT3400.039.7044637
TATGCCG3400.039.7044643
AGGCGAA3400.039.7044631
TCTCGTA3400.039.7044638
ATGCCGT3400.039.7044644
AATCTCG3350.039.6254536
GGCGAAT3350.039.6254532
GCGAATC3300.039.5440433
GCCGTCT403.367786E-739.40534245
CCAAGGC3500.039.21288328
AAGGCGA3450.039.12903230
CAAGGCG3450.039.12903229
CGAATCT3350.038.9538334
GCCCACG3600.038.7486118
CACGAGA3600.038.7486121
Produced by FastQC (version 0.11.2)