FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_6.34100000005bc0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_6.34100000005bc0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC323.1219512195121952No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC171.6585365853658538No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC100.975609756097561TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC80.7804878048780488No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC80.7804878048780488No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC70.6829268292682927No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC60.5853658536585366No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC30.2926829268292683No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC30.2926829268292683No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1951219512195122No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTA20.1951219512195122No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC20.1951219512195122No Hit
ATTTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAG20.1951219512195122No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1951219512195122No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT20.1951219512195122No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGCGAAGCAGTGGTATC20.1951219512195122No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG20.1951219512195122No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG20.1951219512195122No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers