FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_59.34100000005f89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_59.34100000005f89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218387
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC21060.9643431156616465No Hit
GAAGAAGAAGGAGAAGAAGAAGAAGGAGAAGAAGAAGAAGGAGAAGAAGAA2540.11630728935330399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2450.038.56907743
AGGCGAA2350.038.29553231
GGCGAAT2350.038.29553232
CTCGTAT2500.037.79769539
TCTCGTA2500.037.79769538
CGTATGC2550.037.0565641
AATCTCG2500.036.8977536
CGAGACC2500.036.8977523
GCGAATC2450.036.73245233
ATCTCGT2600.036.34393337
GCCCACG2600.036.34393318
TCGTATG2600.036.34393340
TGCCGTC2550.036.1742645
ACGAGAC2650.035.658222
CGAATCT2600.035.478634
ATGCCGT2600.035.478644
CAAGGCG2600.034.62119729
AAGGCGA2600.034.62119730
CGAGCCC2800.034.5514615
CCCACGA2750.034.36153819
Produced by FastQC (version 0.11.2)