##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_58.34100000005f7c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66681 Sequences flagged as poor quality 0 Sequence length 51 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.289482761206344 38.0 38.0 38.0 32.0 38.0 2 36.28853796433767 38.0 38.0 38.0 32.0 38.0 3 36.26670265892833 38.0 38.0 38.0 32.0 38.0 4 36.329494158755864 38.0 38.0 38.0 32.0 38.0 5 36.350249696315295 38.0 38.0 38.0 32.0 38.0 6 38.27965987312728 40.0 38.0 40.0 38.0 40.0 7 38.17408257224697 40.0 38.0 40.0 38.0 40.0 8 38.15155741515574 40.0 38.0 40.0 38.0 40.0 9 38.15884584814265 40.0 38.0 40.0 38.0 40.0 10 38.0983038646691 40.0 38.0 40.0 38.0 40.0 11 38.0836970051439 40.0 38.0 40.0 38.0 40.0 12 37.995186035002476 40.0 38.0 40.0 32.0 40.0 13 37.954139859930116 40.0 38.0 40.0 32.0 40.0 14 37.894872602390485 40.0 38.0 40.0 32.0 40.0 15 37.92819543798083 40.0 38.0 40.0 32.0 40.0 16 37.90850467149563 40.0 38.0 40.0 32.0 40.0 17 37.958368950675606 40.0 38.0 40.0 32.0 40.0 18 37.95103552736162 40.0 38.0 40.0 32.0 40.0 19 37.99040206355634 40.0 38.0 40.0 32.0 40.0 20 38.0409561944182 40.0 38.0 40.0 32.0 40.0 21 38.060931899641574 40.0 38.0 40.0 32.0 40.0 22 38.058697380063286 40.0 38.0 40.0 32.0 40.0 23 38.05983713501597 40.0 38.0 40.0 32.0 40.0 24 38.000749838784664 40.0 38.0 40.0 32.0 40.0 25 37.994481186544895 40.0 38.0 40.0 32.0 40.0 26 37.914083472053505 40.0 38.0 40.0 32.0 40.0 27 37.86238958623896 40.0 38.0 40.0 32.0 40.0 28 37.823008053268545 40.0 38.0 40.0 32.0 40.0 29 37.82522757607114 40.0 38.0 40.0 32.0 40.0 30 37.76915463175417 40.0 38.0 40.0 32.0 40.0 31 37.76101138255275 40.0 38.0 40.0 32.0 40.0 32 37.69694515679129 40.0 38.0 40.0 32.0 40.0 33 37.659513204660996 40.0 38.0 40.0 32.0 40.0 34 37.692446124083325 40.0 38.0 40.0 32.0 40.0 35 37.63580330229001 40.0 38.0 40.0 32.0 40.0 36 37.57467644456442 40.0 38.0 40.0 32.0 40.0 37 37.567133066390724 40.0 38.0 40.0 32.0 40.0 38 37.53336032752958 40.0 38.0 40.0 32.0 40.0 39 37.400383917457745 40.0 38.0 40.0 32.0 40.0 40 37.3872017516234 40.0 38.0 40.0 32.0 40.0 41 37.343651115010275 40.0 38.0 40.0 32.0 40.0 42 37.289392780552184 40.0 38.0 40.0 32.0 40.0 43 37.219627780027295 40.0 38.0 40.0 32.0 40.0 44 37.121083966947104 40.0 38.0 40.0 32.0 40.0 45 37.053868418290065 40.0 38.0 40.0 32.0 40.0 46 36.87639657473643 40.0 38.0 40.0 32.0 40.0 47 36.76924461240833 40.0 38.0 40.0 32.0 40.0 48 36.69162130142019 40.0 38.0 40.0 27.0 40.0 49 36.61207840314332 40.0 38.0 40.0 27.0 40.0 50 36.52629684617807 40.0 38.0 40.0 27.0 40.0 51 36.176302095049564 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.2896452359738717 2201 2 0.2039245081268959 2201 3 0.3068670021161992 2201 4 0.4766468351946571 2201 5 0.18125131862909427 2201 6 0.2866114263373589 2201 7 0.32361458925111464 2201 8 0.13833644808936896 2201 9 -0.15174465872923548 2201 10 -0.9656241876123346 2201 11 -0.1555367061301638 2201 12 0.30605826917844325 2201 13 -0.34133576612398997 2201 14 0.5891849912738607 2201 15 -0.4781089516474566 2201 16 0.1461314087741883 2201 17 -0.6591333445519183 2201 18 0.19487091718978178 2201 19 0.45836854317055753 2201 20 -0.023405295437470386 2201 21 0.4109310889039648 2201 22 -0.04435309961175449 2201 23 -0.4193662208345117 2201 24 0.1643185768718567 2201 25 0.18458126044409084 2201 26 -0.08302111941947032 2201 27 0.5812303774929859 2201 28 0.10370430671972741 2201 29 0.1330330386612104 2201 30 0.4092650733393768 2201 31 0.4982952268020995 2201 32 0.4073911731825888 2201 33 0.2511673177184903 2201 34 0.04441171987257064 2201 35 0.15063151217780302 2201 36 0.10791556776277389 2201 37 0.4742627244552651 2201 38 -0.029718807397998148 2201 39 0.7947959537848632 2201 40 0.37180158322907886 2201 41 0.1913510239508156 2201 42 -0.4153904105320265 2201 43 -0.4826985715156553 2201 44 -0.47949544975521263 2201 45 -0.2906484252712076 2201 46 -0.717930931852031 2201 47 -1.1068276378572008 2201 48 -0.652831298664502 2201 49 -0.35924276150487344 2201 50 -0.028885753305104345 2201 51 0.12417447373995572 1101 1 0.33401067800462414 1101 2 0.5480105296322719 1101 3 0.17783474405167965 1101 4 0.9820231792806808 1101 5 0.2135093831452224 1101 6 0.14682648010079902 1101 7 -0.5581058408564132 1101 8 0.568443974971089 1101 9 0.009545663851405095 1101 10 0.24942957582852188 1101 11 0.21005469171930002 1101 12 0.03724106487736378 1101 13 0.21780401882224254 1101 14 0.5569269267577326 1101 15 0.9842566397503987 1101 16 0.4902174302795643 1101 17 1.0505440748029216 1101 18 0.9583117774048375 1101 19 0.9637448872565741 1101 20 0.557239865852857 1101 21 0.43243646524805257 1101 22 0.7190877606033013 1101 23 0.9247198006708643 1101 24 0.6374368564417452 1101 25 0.30286083033656297 1101 26 0.669667052623538 1101 27 0.7210153237295458 1101 28 0.6090806508057511 1101 29 0.7351835762956185 1101 30 1.0866844281780885 1101 31 0.9391554418558599 1101 32 0.7407245065159245 1101 33 0.42321032847117834 1101 34 0.7433364510553702 1101 35 0.7420293616401636 1101 36 0.32296933120363747 1101 37 0.8183487459606411 1101 38 0.6584532356127539 1101 39 0.41845186776335197 1101 40 0.7804037337667182 1101 41 0.4171574755637195 1101 42 0.8426741055970126 1101 43 0.6893444392370327 1101 44 0.9828701416426355 1101 45 1.010426843546 1101 46 1.0670153047071054 1101 47 -0.08532226151311306 1101 48 0.4977063357440983 1101 49 0.33968196967791897 1101 50 1.121651881103503 1101 51 0.758583075890499 2202 1 0.14046229090784834 2202 2 0.5802685941484 2202 3 0.45740463652479946 2202 4 0.04653930831293707 2202 5 0.3640470175538226 2202 6 -0.01446384247984156 2202 7 0.334367277423155 2202 8 0.4286590287345291 2202 9 0.5901908251417325 2202 10 0.3999672102371221 2202 11 -0.048009824409732005 2202 12 0.49960665627521195 2202 13 0.47586853495127457 2202 14 0.6429484321340766 2202 15 0.4036114784600713 2202 16 0.6730131292042927 2202 17 0.8032322468459299 2202 18 0.5174515623510771 2202 19 0.21105671521356584 2202 20 0.6862721239173695 2202 21 0.6259848523448284 2202 22 0.5255393735065255 2202 23 0.29031119852032816 2202 24 0.5299099747213134 2202 25 0.6576995400139793 2202 26 0.5191294182149377 2202 27 0.1403701624392255 2202 28 0.10370430671972741 2202 29 0.8319577698440028 2202 30 0.5167919550598086 2202 31 0.6058221085225242 2202 32 0.14932665705355674 2202 33 0.17589850051418665 2202 34 0.07666978438869876 2202 35 -0.36549752008026104 2202 36 0.03264675055847732 2202 37 0.6355530470359056 2202 38 1.2283457087310339 2202 39 0.5259787494837838 2202 40 -0.004542502792425296 2202 41 -0.18499306207068855 2202 42 0.939448299145397 2202 43 1.1947207833230564 2202 44 0.8645905717501634 2202 45 1.268491359675032 2202 46 1.024004552018937 2202 47 0.5490863406374231 2202 48 1.2503945077871137 2202 49 0.09237014172093438 2202 50 0.27218951551210324 2202 51 1.0811637210517873 1102 1 -0.17668658393972692 1102 2 0.5138679733746514 1102 3 0.4693767017060395 1102 4 -0.12494944016283682 1102 5 0.08691587909910936 1102 6 0.04971972918627188 1102 7 -0.12888513361775011 1102 8 -1.0269445628850207 1102 9 -0.24408475406234942 1102 10 0.07927105681732627 1102 11 0.030917123822170822 1102 12 0.12060211132454413 1102 13 0.36401840747095093 1102 14 -0.4177987134152019 1102 15 0.24597928690702986 1102 16 0.4845639550550871 1102 17 0.4938430438750885 1102 18 0.012075218265053422 1102 19 -0.07438835739479543 1102 20 0.20295541845234766 1102 21 -0.20607367775161833 1102 22 0.5656679623547731 1102 23 -1.230362784408392 1102 24 -0.8561891273737956 1102 25 0.2877848964046237 1102 26 0.11861949692018214 1102 27 0.16098871914025636 1102 28 0.012694440851198863 1102 29 0.15365159536224127 1102 30 -0.07560356650099465 1102 31 -0.8543264337676888 1102 32 -0.9309305206429315 1102 33 -0.10389309686969028 1102 34 -0.07675001385982938 1102 35 0.1500772499008889 1102 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-0.1611133753484424 1213 32 -0.8469099020862245 1213 33 -0.7423810693782826 1213 34 -0.9104269898048472 1213 35 -0.904476014703917 1213 36 -0.8875145397641049 1213 37 -0.75933942608237 1213 38 -0.9291811729893951 1213 39 -1.0191825408387984 1213 40 -1.0142199221472623 1213 41 -0.305960804006169 1213 42 -0.6196914858008427 1213 43 0.09095734246283627 1213 44 -0.027882546529404806 1213 45 0.21042684354600283 1213 46 -0.3233072759380491 1213 47 -0.33774161635182764 1213 48 -0.555519470707516 1213 49 -0.5127373851607899 1213 50 -1.597434140401873 1213 51 -1.272330902604125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 3.0 17 1.0 18 3.0 19 8.0 20 15.0 21 15.0 22 45.0 23 77.0 24 129.0 25 239.0 26 341.0 27 495.0 28 619.0 29 840.0 30 1127.0 31 1303.0 32 1632.0 33 2019.0 34 2428.0 35 3026.0 36 4055.0 37 5511.0 38 10088.0 39 32662.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.574551073373435 22.510088660195922 13.699575451176885 24.215784815253755 2 13.127071491774023 24.160530301894152 38.281917845198635 24.430480361133192 3 20.416610428757817 28.565858340456803 29.73260748938978 21.284923741395602 4 12.232869932964412 22.310703198812256 34.25863439360538 31.19779247461796 5 12.606289647725738 36.1782216823383 34.578065715871084 16.637422954064878 6 30.600920802027566 39.834435596346786 14.074473988092558 15.490169613533089 7 21.317916647920697 36.880070784781275 19.552796148828 22.24921641847003 8 22.72311453037597 36.16772393935304 21.415395689926665 19.693765840344327 9 22.08875091855251 21.553366026304342 21.26692761056373 35.090955444579414 10 14.797318576506052 32.124593212459324 28.918282569247612 24.159805641787017 11 27.618890505256527 26.449106915220682 24.15153196658618 21.780470612936607 12 20.939997900451406 29.291702284008934 28.828301915088257 20.939997900451406 13 25.142094449693314 24.50323180516189 28.603350279689867 21.751323465454927 14 19.695265517913647 24.384757277185404 29.435671330663908 26.48430587423704 15 21.940282839189575 29.360687452197777 26.55179136485656 22.147238343756094 16 20.11217588218533 32.877431352259265 25.96691711282074 21.04347565273466 17 17.167066586682665 33.52579484103179 29.904019196160768 19.403119376124774 18 19.395620875824836 28.147870425914817 34.500599880024 17.955908818236352 19 17.126317841664044 30.52893627870008 32.65547907199952 19.689266807636358 20 17.48923981344011 28.105457326674767 33.20736041751024 21.197942442374888 21 15.238223781886894 31.22478667086576 32.95391490829472 20.583074638952624 22 15.800602870382868 34.51957829066751 33.123378473628165 16.556440365321457 23 15.77960738441235 30.942847287833114 33.160870412861236 20.1166749148933 24 18.607999280154765 31.92213674060077 33.699254660248045 15.770609318996415 25 16.787390710997137 33.59127787525682 33.19536299695565 16.42596841679039 26 18.092381523695263 31.543191361727658 34.04919016196761 16.315236952609478 27 16.19501807111471 34.560069585039216 33.627270136920565 15.617642206925511 28 15.295211529521152 31.412246367031088 33.73674659948111 19.555795503966646 29 15.593647365816349 31.80216253505496 34.0216853376524 18.582504761476283 30 15.745587066392716 34.93003794298055 33.61328154291456 15.711093447712173 31 16.21901291222387 34.49408377198902 33.02290007648356 16.264003239303552 32 18.768464780072282 32.055608044270485 33.26284848757517 15.913078688082063 33 18.39954409801893 32.49051453937403 33.14737331473733 15.962568047869707 34 16.133531290772485 32.70646810935649 32.90742490364572 18.25257569622531 35 18.393545387741636 32.883430062536554 32.8864294176752 15.836595132046611 36 15.541158650890058 35.8797858460431 32.95541458586404 15.623640917202803 37 15.389691216388476 35.33840224351764 33.402318501522174 15.869588038571708 38 15.235224426748248 32.49951260478997 35.9592687572172 16.30599421124458 39 15.716620926500802 32.1095964367661 33.15037266987598 19.023409966857123 40 15.664132211574511 32.055608044270485 36.05524812165385 16.225011622501164 41 15.971566113285643 32.02111546017606 33.04089620731543 18.966422219222867 42 18.441535069959958 32.09459966107287 33.339332043610625 16.12453322535655 43 15.778107706843029 32.48451582909674 35.52436226211365 16.21301420194658 44 15.478172192978509 34.92149187924596 32.86843328684333 16.7319026409322 45 15.563653814429898 32.14258934329119 35.80480196757697 16.48895487470194 46 19.06989997150613 31.84565318456532 32.000119974205546 17.08432686972301 47 15.424646440408523 32.22603818293616 32.356514044901694 19.992801331753626 48 15.563653814429898 32.07360417510235 32.09609933864219 20.266642671825558 49 18.525517013842023 32.010617717190804 32.243067740435805 17.220797528531364 50 15.110751188494472 32.414030983338584 34.737031538219284 17.73818628994766 51 15.174192774336747 32.34751570959373 31.812114758769628 20.6661767572999 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1696.0 1 956.5 2 217.0 3 365.5 4 514.0 5 494.0 6 474.0 7 514.5 8 555.0 9 579.5 10 604.0 11 1008.0 12 1412.0 13 2005.5 14 2599.0 15 2964.5 16 3330.0 17 2548.0 18 1766.0 19 1440.5 20 1115.0 21 1401.5 22 1688.0 23 1495.0 24 1302.0 25 1134.0 26 1313.5 27 1661.0 28 1479.5 29 1298.0 30 1405.5 31 1513.0 32 1792.0 33 2071.0 34 2322.0 35 2573.0 36 2649.0 37 2725.0 38 2786.0 39 2847.0 40 2964.0 41 3081.0 42 3047.0 43 3013.0 44 3095.0 45 3177.0 46 3173.5 47 3170.0 48 3154.0 49 3138.0 50 3091.5 51 3045.0 52 3110.5 53 3176.0 54 3831.0 55 4486.0 56 3276.0 57 2066.0 58 1849.0 59 1632.0 60 1449.5 61 1267.0 62 1101.5 63 936.0 64 780.5 65 625.0 66 498.5 67 372.0 68 303.0 69 234.0 70 185.0 71 136.0 72 121.5 73 107.0 74 75.0 75 37.0 76 31.0 77 23.5 78 16.0 79 9.5 80 3.0 81 2.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03299290652509711 2 0.0029993551386451912 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0029993551386451912 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0014996775693225956 18 0.0014996775693225956 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0014996775693225956 27 0.0 28 0.0 29 0.0 30 0.0029993551386451912 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0029993551386451912 48 0.0 49 0.0 50 0.0 51 0.0029993551386451912 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 66681.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.79796546409763 #Duplication Level Percentage of deduplicated Percentage of total 1 95.36361445864672 67.51549882974793 2 3.399651484732906 4.813768168121769 3 0.49026477255305195 1.0412924530642398 4 0.18608946135392315 0.5269902103267032 5 0.0985288394338553 0.3487820685727855 6 0.10143085973842328 0.4308659102852773 7 0.045155353321716656 0.22378350014930154 8 0.019745404108180983 0.11183475505005189 9 0.024110546438232273 0.1536279870649042 >10 0.16553156669467664 2.809283429570082 >50 0.03599890471007728 1.8395135552830515 >100 0.05717319404444306 8.365341353561874 >500 0.0063525771119131965 3.9008276333693277 >1k 0.0063525771119131965 7.91859014583274 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC 1879 2.817894152757157 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1780 2.66942607339422 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1623 2.433976695010573 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 991 1.4861804711986923 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 931 1.3961998170393366 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 680 1.019780747139365 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 464 0.6958503921656845 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 456 0.6838529716111036 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 392 0.5878736071744575 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 343 0.5143894062776503 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 318 0.47689746704458535 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 304 0.4559019810740691 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 299 0.44840359322745615 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 241 0.36142229420674554 No Hit TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT 239 0.35842293906810035 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 182 0.2729413176167124 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 178 0.26694260733942204 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 175 0.26244357463145424 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 169 0.25344550921551867 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 158 0.23694905595297008 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 156 0.23394970081432492 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 143 0.2144538924131312 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 139 0.2084551821358408 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 0.2084551821358408 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 138 0.2069555045665182 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 136 0.203956149427873 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 130 0.19495808401193743 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 116 0.1739625980414211 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 116 0.1739625980414211 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA 114 0.1709632429027759 No Hit GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112 0.1679638877641307 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112 0.1679638877641307 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 111 0.16646421019480812 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA 98 0.14696840179361437 No Hit ATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTTC 91 0.1364706588083562 RNA PCR Primer, Index 46 (95% over 22bp) GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 88 0.13197162610038843 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA 88 0.13197162610038843 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 86 0.1289722709617432 No Hit ATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.12597291582309805 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 79 0.11847452797648504 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 75 0.11247581769919467 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 73 0.10947646256054948 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 67 0.1004783971446139 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0014996775693225956 17 0.0 0.0 0.0014996775693225956 18 0.0 0.0 0.0029993551386451912 19 0.0 0.0 0.0059987102772903825 20 0.0 0.0 0.0059987102772903825 21 0.0 0.0 0.007498387846612978 22 0.0 0.0 0.01349709812390336 23 0.0 0.0 0.017996130831871147 24 0.0 0.0 0.031493228955774506 25 0.0 0.0 0.03749193923306489 26 0.0 0.0 0.059987102772903825 27 0.0 0.0 0.07498387846612978 28 0.0 0.0 0.10947646256054948 29 0.0 0.0 0.16196517748684033 30 0.0 0.0 0.2054558269971956 31 0.0 0.0 0.3764190698999715 32 0.0 0.0 0.5518813455107152 33 0.0 0.0 0.7408407192453622 34 0.0 0.0 1.001784616307494 35 0.0 0.0 1.2342346395524961 36 0.0 0.0 1.5551656393875317 37 0.0 0.0 1.976575036367181 38 0.0 0.0 2.381487980084282 39 0.0 0.0 2.8133951200491896 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGAATC 250 0.0 39.6 33 ATCTCGT 265 0.0 39.056602 37 CGTATGC 265 0.0 39.056602 41 TCTCGTA 265 0.0 39.056602 38 AGGCGAA 255 0.0 38.82353 31 AATCTCG 255 0.0 38.82353 36 CGAATCT 255 0.0 38.82353 34 GGCGAAT 255 0.0 38.82353 32 AAGGCGA 255 0.0 38.82353 30 GTATGCC 275 0.0 38.454544 42 GCCCACG 270 0.0 38.333332 18 CTCGTAT 270 0.0 38.333332 39 CACGAGA 270 0.0 38.333332 21 TCGTATG 270 0.0 38.333332 40 CCACGAG 270 0.0 38.333332 20 TGCCGTC 270 0.0 38.333332 45 GAATCTC 260 0.0 38.076927 35 GACCAAG 260 0.0 38.076927 26 CAAGGCG 260 0.0 38.076927 29 CGAGACC 260 0.0 38.076927 23 >>END_MODULE