FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_58.34100000005f7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_58.34100000005f7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66681
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC18792.817894152757157No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17802.66942607339422No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16232.433976695010573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9911.4861804711986923No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9311.3961998170393366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6801.019780747139365No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4640.6958503921656845No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4560.6838529716111036No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.5878736071744575No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.5143894062776503No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3180.47689746704458535No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.4559019810740691No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2990.44840359322745615No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.36142229420674554No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT2390.35842293906810035No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1820.2729413176167124No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.26694260733942204No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.26244357463145424No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.25344550921551867No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA1580.23694905595297008No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.23394970081432492No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.2144538924131312No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.2084551821358408No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.2084551821358408No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.2069555045665182No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA1360.203956149427873No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.19495808401193743No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.1739625980414211No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA1160.1739625980414211No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA1140.1709632429027759No Hit
GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.1679638877641307No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.1679638877641307No Hit
GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.16646421019480812No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA980.14696840179361437No Hit
ATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTTC910.1364706588083562RNA PCR Primer, Index 46 (95% over 22bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.13197162610038843No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA880.13197162610038843No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.1289722709617432No Hit
ATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.12597291582309805No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.11847452797648504No Hit
GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.11247581769919467No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.10947646256054948No Hit
CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.1004783971446139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC2500.039.633
ATCTCGT2650.039.05660237
CGTATGC2650.039.05660241
TCTCGTA2650.039.05660238
AGGCGAA2550.038.8235331
AATCTCG2550.038.8235336
CGAATCT2550.038.8235334
GGCGAAT2550.038.8235332
AAGGCGA2550.038.8235330
GTATGCC2750.038.45454442
GCCCACG2700.038.33333218
CTCGTAT2700.038.33333239
CACGAGA2700.038.33333221
TCGTATG2700.038.33333240
CCACGAG2700.038.33333220
TGCCGTC2700.038.33333245
GAATCTC2600.038.07692735
GACCAAG2600.038.07692726
CAAGGCG2600.038.07692729
CGAGACC2600.038.07692723
Produced by FastQC (version 0.11.2)