FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_57.34100000005f53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_57.34100000005f53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318315
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC8900.279597254292132No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGCG250.002105150435.9988677
GGCGAAT754.7293724E-1132.99896232
CTACGCT300.00514252329.999064
TATGCCG901.2732926E-1129.99905643
TCGTATG901.2732926E-1129.99905640
ACGAGAC901.2732926E-1129.99905622
AAGGCGA852.0190782E-1029.1213130
GCCCACG952.3646862E-1128.4201618
CGTATGC952.3646862E-1128.4201641
CACGAGA952.3646862E-1128.4201621
CCCACGA952.3646862E-1128.4201619
CGAGCCC952.3646862E-1128.4201615
CAAGGCG903.929017E-1027.50345429
CTCGTAT1004.5474735E-1126.99915139
ATGCCGT1105.456968E-1226.59007544
GCGAATC957.366907E-1026.05181333
AGGCGAA957.366907E-1026.05181331
CCACGAG1058.367351E-1125.71347820
TGCCGTC1159.094947E-1225.43398345
ACACCGT450.001224831224.9992146
Produced by FastQC (version 0.11.2)