FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_56.34100000005f46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_56.34100000005f46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6558
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC484973.94022567856054No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT2894.406831351021653No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT901.3723696248856359No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC831.2656297651723085No Hit
CTTATACACATCTGACGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC560.8539188777066178No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC500.7624275693809088No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG320.48795364440378164No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC270.41171088746569073No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC270.41171088746569073RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC250.3812137846904544No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA250.3812137846904544No Hit
CTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG240.36596523330283626No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA210.32021957913998167No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACG180.2744739249771272No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATATCGTATGCCGTC130.19823116803903632No Hit
CTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA130.19823116803903632No Hit
CGGAGATGTGTATAAGAGACAGGCGTCAGATGTGTATAAGAGACAGCTTAT100.15248551387618176No Hit
CTTATACACATCTACGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC90.1372369624885636No Hit
TTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA90.1372369624885636No Hit
ATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACG90.1372369624885636No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGATAACCT90.1372369624885636No Hit
ATACACATCTGACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT80.12198841110094541No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTA70.10673985971332722No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC70.10673985971332722No Hit
CTTATACACATCTGACGCTCTGTCTCTTATACACATCTCCGAGCCCACGAG70.10673985971332722No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT452.7830538E-1044.94656845
AACCTAT5350.042.00613432
TAACCTA5350.042.00613431
ACCTATC5300.041.97839433
CTTATAC5800.041.84681
AGACGAT5550.041.70717225
GACGATA5550.041.70717226
ACGATAA5550.041.70717227
GAGACGA5550.041.70717224
CGATAAC5500.041.67772328
GATAACC5500.041.67772329
TATCTCG5500.041.67772336
ATAACCT5400.041.6171930
CTATCTC5400.041.6171935
CCTATCT5350.041.5860734
TGCCGTC5600.041.3347945
CGAGACG5750.041.03816623
GTATGCC5650.040.96899442
TATGCCG5650.040.96899443
ATGCCGT5650.040.96899444
Produced by FastQC (version 0.11.2)