FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_55.34100000005f39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_55.34100000005f39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128624
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC56314.377876601567359No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC19301.5004975743251647No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC13461.0464610026122652No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.6958265953476801No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG8820.6857196168677696No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC8510.6616183604925986No Hit
CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG7500.5830949123025252No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7470.580762532653315No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACA6770.5263403408384127No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC6330.4921321059833313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.4470394327652693No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT5180.40272421943027736No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA5150.4003918397810673No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACA4960.38562010200273666No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4310.3350852096031845No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA4090.31798109217564374No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCC3830.2977671352158229No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.29698967533275283No Hit
TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA3750.2915474561512626No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACATC3470.26977857942530165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.25733922129618114No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA3270.25422938176390103No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC3190.24800970269934072No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT3110.24179002363478044No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3110.24179002363478044No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.23012812538872993No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACA2720.21146908819504912No Hit
AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA2670.207581788779699No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG2650.2060268690135589No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCC2630.20447194924741882No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACATC2310.17959323298917776No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2300.17881577310610772No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCG2280.17726085333996766No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA2280.17726085333996766No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT2270.17648339345689762No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2240.17415101380768752No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTC2230.1733735539246175No Hit
TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA2180.16948625450926733No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.1640440353277771No Hit
AAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGA2110.1640440353277771No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.1593792760293569No Hit
AAAAAGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACATCTCCGA1950.15160467719865656No Hit
GTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCA1800.13994277895260604No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGA1780.13838785918646598No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTC1780.13838785918646598No Hit
TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC1670.1298358004726956No Hit
AAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAG1660.12905834058962556No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.12828088070655552No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.12828088070655552No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC1610.1251710411742754No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.12128374175892524No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGA1490.115841522577435No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTATAC1450.11273168304515487No Hit
TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATC1440.11195422316208484No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCACTGTCTCTTATACACATC1430.1111767632790148No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCG1410.10962184351287474No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCT1380.10728946386366464No Hit
AAAAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTC1360.10573454409752456No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA1360.10573454409752456No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTAT1340.1041796243313845No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGA1290.10029232491603433No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACT253.8714465E-545.023
TGCCGTC7450.042.88590645
ATGCCGT8050.039.6894444
TATCTCG8050.039.13043236
TCTCGTA8300.039.03614438
ACGAGAC8300.039.03614422
CCACGAG8300.039.03614420
CGATAAC7850.038.98089228
ATCTCGT8350.038.80239537
CTCGTAT8350.038.80239539
GAGACGA7950.038.77358624
CGAGACG7950.038.77358623
TCGTATG8300.038.7650640
AGACGAT7900.038.73417725
TAACCTA7900.038.73417731
TATGCCG8250.038.72727243
CACGAGA8400.038.5714321
CGTATGC8350.038.53293641
ACCTATC8000.038.5312533
CCTATCT8000.038.5312534
Produced by FastQC (version 0.11.2)