##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_54.34100000005f2c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14145 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.19632378932485 38.0 38.0 38.0 32.0 38.0 2 36.325061859314246 38.0 38.0 38.0 32.0 38.0 3 36.24673029338989 38.0 38.0 38.0 32.0 38.0 4 36.369812654648285 38.0 38.0 38.0 32.0 38.0 5 36.388617886178864 38.0 38.0 38.0 32.0 38.0 6 38.3117709437964 40.0 38.0 40.0 38.0 40.0 7 38.126546482856135 40.0 38.0 40.0 38.0 40.0 8 38.00629197596324 40.0 38.0 40.0 38.0 40.0 9 38.184022622834924 40.0 38.0 40.0 38.0 40.0 10 38.04255920820078 40.0 38.0 40.0 38.0 40.0 11 38.097914457405444 40.0 38.0 40.0 38.0 40.0 12 37.81689642983386 40.0 38.0 40.0 32.0 40.0 13 37.91778013432308 40.0 38.0 40.0 32.0 40.0 14 37.89699540473666 40.0 38.0 40.0 32.0 40.0 15 37.90427712972782 40.0 38.0 40.0 32.0 40.0 16 37.76295510781195 40.0 38.0 40.0 32.0 40.0 17 37.72463768115942 40.0 38.0 40.0 32.0 40.0 18 37.576670201484625 40.0 38.0 40.0 32.0 40.0 19 37.74202898550725 40.0 38.0 40.0 32.0 40.0 20 37.75574407917992 40.0 38.0 40.0 32.0 40.0 21 37.82785436550018 40.0 38.0 40.0 32.0 40.0 22 37.89232944503358 40.0 38.0 40.0 32.0 40.0 23 37.819936373276775 40.0 38.0 40.0 32.0 40.0 24 37.688016967126195 40.0 38.0 40.0 32.0 40.0 25 37.67253446447508 40.0 38.0 40.0 32.0 40.0 26 37.60607988688582 40.0 38.0 40.0 32.0 40.0 27 37.47394839165783 40.0 38.0 40.0 32.0 40.0 28 37.5558854718982 40.0 38.0 40.0 32.0 40.0 29 37.51728525980912 40.0 38.0 40.0 32.0 40.0 30 37.38918345705196 40.0 38.0 40.0 32.0 40.0 31 37.43513609049134 40.0 38.0 40.0 32.0 40.0 32 37.50250972074938 40.0 38.0 40.0 32.0 40.0 33 37.62424885118416 40.0 38.0 40.0 32.0 40.0 34 37.65577942735949 40.0 38.0 40.0 32.0 40.0 35 37.64503358077059 40.0 38.0 40.0 32.0 40.0 36 37.61399787910923 40.0 38.0 40.0 32.0 40.0 37 37.475150229763166 40.0 38.0 40.0 32.0 40.0 38 37.42834924001414 40.0 38.0 40.0 32.0 40.0 39 37.3349593495935 40.0 38.0 40.0 32.0 40.0 40 37.23966065747614 40.0 38.0 40.0 32.0 40.0 41 37.43089430894309 40.0 38.0 40.0 32.0 40.0 42 37.422198656769176 40.0 38.0 40.0 32.0 40.0 43 37.500035348179566 40.0 38.0 40.0 32.0 40.0 44 37.380346412159774 40.0 38.0 40.0 32.0 40.0 45 37.330646871686106 40.0 38.0 40.0 32.0 40.0 46 37.15956168257335 40.0 38.0 40.0 32.0 40.0 47 37.16486390950866 40.0 38.0 40.0 32.0 40.0 48 37.07720042417815 40.0 38.0 40.0 32.0 40.0 49 36.994697773064686 40.0 38.0 40.0 32.0 40.0 50 36.952279957582185 38.0 38.0 40.0 32.0 40.0 51 36.36578296217745 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.7648578401760702 2201 2 0.08999430824802346 2201 3 0.36452259063411674 2201 4 -0.018882355076321744 2201 5 0.7365845712849648 2201 6 -0.12611274364574143 2201 7 1.1048624808535834 2201 8 1.1374216281270506 2201 9 0.537653559463287 2201 10 0.9327299925736483 2201 11 0.2420330587024253 2201 12 0.225643358563012 2201 13 -0.6288823200495415 2201 14 0.6158200360535702 2201 15 -0.22943870885390538 2201 16 1.0329843230568017 2201 17 0.7848762458673946 2201 18 1.0552085696502331 2201 19 -0.2200521260849726 2201 20 0.6980809967867287 2201 21 -0.23627704075837386 2201 22 0.5063261796816647 2201 23 0.20232542469382508 2201 24 0.6835344414398392 2201 25 -0.6559655098950259 2201 26 -0.22913000573514353 2201 27 0.4529785743252077 2201 28 1.1407492702885662 2201 29 1.2883293720382483 2201 30 0.8866218162926174 2201 31 1.0431065954954803 2201 32 -1.4547628528786731 2201 33 -0.4955240331109678 2201 34 -1.8031791143055713 2201 35 -0.1328637955688663 2201 36 -1.5196455042317254 2201 37 0.8136928933488861 2201 38 -0.03821239542692467 2201 39 -0.43963282628062217 2201 40 0.37980316404126313 2201 41 1.0441035914302645 2201 42 1.0127142447425541 2201 43 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1212 43 -0.12519845269645913 1212 44 0.6561702839011829 1212 45 1.4841141110485125 1212 46 1.110754050878171 1212 47 1.3337589282229416 1212 48 0.18991971314989797 1212 49 -0.4014076111763245 1212 50 0.006330406078248529 1212 51 -0.26577067069786153 1213 1 -0.6865591638725164 1213 2 0.9624639438755551 1213 3 -1.1415502838598073 1213 4 -0.28608883280911357 1213 5 0.015936798005611763 1213 6 -0.9378536343339974 1213 7 0.03401227842443433 1213 8 -1.4617686552737652 1213 9 -1.1222654688767904 1213 10 0.06228464844409842 1213 11 -0.8612058076943399 1213 12 -0.3310368033803073 1213 13 -0.9446717937337539 1213 14 -2.9246657939059446 1213 15 -1.0715439720118027 1213 16 -2.9143840979958284 1213 17 -1.7515610010961637 1213 18 -1.1290019566655545 1213 19 -0.5925217617125043 1213 20 -1.0590040234561897 1213 21 0.36291324264244196 1213 22 0.7006581634873328 1213 23 0.4857262344104285 1213 24 0.7726032673507675 1213 25 -0.25110721029988525 1213 26 -0.1400611798242153 1213 27 0.5541931492239911 1213 28 0.10633631482297545 1213 29 -0.5537758911196491 1213 30 -1.2307870905899776 1213 31 -0.12693389033447744 1213 32 -1.8677183184657196 1213 33 -0.8214349642850536 1213 34 0.3405455820507086 1213 35 -0.6105965890911307 1213 36 -1.047985585203385 1213 37 -2.2267929366106287 1213 38 -1.9208034885443297 1213 39 -1.4497542837704955 1213 40 -1.8044073622745245 1213 41 0.301188611673183 1213 42 -0.6674476985772841 1213 43 -0.11970394720194832 1213 44 0.6012252289561317 1213 45 -1.3620397351053342 1213 46 -1.3178173776932596 1213 47 -0.9574498629858539 1213 48 -1.5051352319050508 1213 49 -0.8189900287587406 1213 50 -2.0183948686470288 1213 51 0.041921636994445066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 1.0 22 7.0 23 10.0 24 31.0 25 43.0 26 67.0 27 94.0 28 140.0 29 201.0 30 271.0 31 309.0 32 358.0 33 415.0 34 524.0 35 614.0 36 845.0 37 1259.0 38 2351.0 39 6604.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.43611680690094 24.612882698154564 11.77260835749134 39.17839213745316 2 9.027925061859314 26.30611523506539 52.046659597030754 12.61930010604454 3 11.728525980911982 30.22269353128314 44.892188052315305 13.156592435489573 4 10.759985860728174 47.28172499116295 25.45775892541534 16.500530222693534 5 7.578649699540474 34.217037822552136 49.31778013432308 8.886532343584305 6 22.86320254506893 56.231884057971016 10.950866030399434 9.954047366560623 7 14.004948745139625 27.281724991162953 22.70060091905267 36.01272534464475 8 13.467656415694593 57.40544361965358 14.627076705549664 14.499823259102158 9 15.100742311770944 17.836691410392362 13.44644750795334 53.61611876988335 10 9.671261930010605 46.34146341463415 29.218805231530574 14.768469423824673 11 19.244909502262445 18.417703619909503 40.2927036199095 22.04468325791855 12 14.125132555673384 18.925415341109932 28.56839872746554 38.38105337575115 13 23.244962884411454 16.083421703782257 42.70767055496642 17.963944856839873 14 13.107104984093318 17.850830682219865 20.282785436550018 48.759278897136795 15 23.244962884411454 18.960763520678686 19.64651820431248 38.14775539059738 16 38.49688914027149 23.34558823529412 23.798076923076923 14.359445701357465 17 16.05514316012725 43.90243902439025 26.058677978084127 13.983739837398373 18 42.85613290915518 19.427359490986216 23.775185577942736 13.941322021915873 19 13.290915517850832 28.073524213503003 21.90880169671262 36.726758571933544 20 12.640509013785788 18.78402262283492 28.27854365500177 40.29692470837752 21 11.975963237893248 26.447507953340406 21.993637327677625 39.58289148108872 22 11.580063626723224 44.043831742665255 21.859314245316366 22.516790385295156 23 13.941322021915873 18.289148108872393 21.689642983386356 46.079886885825374 24 36.28137150936727 23.775185577942736 22.68646164722517 17.25698126546483 25 17.023683280311065 41.371509367267585 23.2096147048427 18.39519264757865 26 38.65676917638742 20.848356309650054 22.629904559915165 17.864969954047368 27 13.283845881937081 43.01166489925769 26.3768115942029 17.327677624602334 28 18.190173206079887 18.451749734888654 22.76422764227642 40.593849416755035 29 35.998586072817254 20.00706963591375 27.2180982679392 16.7762460233298 30 13.638291855203619 41.94004524886878 24.660633484162897 19.761029411764707 31 12.951572993990808 21.505832449628844 51.83457051961824 13.708024036762106 32 11.968893601979499 46.41215977377165 23.951926475786497 17.667020148462353 33 11.544715447154472 45.10427712972782 26.822198656769174 16.528808766348533 34 11.69317780134323 22.149169317780135 26.291975963237896 39.865676917638744 35 11.83457051961824 24.241781548250266 22.849063273241427 41.07458465889007 36 11.417462000706964 22.516790385295156 47.182750088370454 18.882997525627427 37 11.304347826086957 47.69176387416048 24.185224460940262 16.818663838812302 38 11.283138918345704 20.44538706256628 45.083068221986565 23.18840579710145 39 11.000353481795687 22.757158006362673 27.423117709437967 38.819370802403675 40 11.898197242841993 15.991516436903499 47.86143513609049 24.248851184164018 41 12.852598091198303 18.522446094026158 24.694238246730293 43.930717568045246 42 35.36231884057971 16.578296217744786 28.561329091551784 19.49805585012372 43 12.32237539766702 17.412513255567337 45.6910569105691 24.574054436196533 44 13.09296571226582 40.50194414987628 26.871686108165427 19.533404029692473 45 14.542241074584657 18.776952986921174 46.249558147755394 20.431247790738777 46 36.60657476139979 18.3881230116649 21.951219512195124 23.05408271474019 47 17.278190173206077 17.073170731707318 21.074584658890068 44.57405443619653 48 12.753623188405797 19.703075291622483 19.936373276776244 47.606928243195476 49 37.659950512548605 17.88617886178862 20.95440084835631 23.49946977730647 50 11.254860374690704 18.953693884764935 43.69034994697773 26.101095793566632 51 11.05691056910569 19.51219512195122 21.25132555673383 48.17956875220926 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 132.0 1 69.0 2 6.0 3 7.5 4 9.0 5 10.5 6 12.0 7 8.0 8 4.0 9 8.5 10 13.0 11 23.0 12 33.0 13 44.5 14 56.0 15 61.0 16 66.0 17 52.0 18 38.0 19 35.5 20 33.0 21 25.5 22 18.0 23 13.5 24 9.0 25 11.0 26 15.5 27 18.0 28 24.5 29 31.0 30 59.5 31 88.0 32 171.0 33 254.0 34 448.0 35 642.0 36 831.0 37 1020.0 38 1212.0 39 1404.0 40 1399.0 41 1394.0 42 1230.5 43 1067.0 44 1395.5 45 1724.0 46 1191.5 47 659.0 48 761.0 49 863.0 50 2289.5 51 3716.0 52 2003.5 53 291.0 54 230.0 55 169.0 56 133.0 57 97.0 58 95.5 59 94.0 60 76.5 61 59.0 62 53.5 63 48.0 64 37.0 65 26.0 66 19.0 67 12.0 68 11.5 69 11.0 70 8.0 71 5.0 72 5.0 73 5.0 74 4.5 75 2.5 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014139271827500884 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.007069635913750442 12 0.0 13 0.0 14 0.0 15 0.0 16 0.007069635913750442 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.007069635913750442 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 14145.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.29338989042065 #Duplication Level Percentage of deduplicated Percentage of total 1 87.51757098678662 44.01555319901025 2 8.096710711273545 8.14422057264051 3 2.3053134664042734 3.4782608695652173 4 0.64661231374754 1.3008130081300813 5 0.4076468934495361 1.025097207493814 6 0.11245431543435479 0.3393425238600212 7 0.09839752600506045 0.34641215977377166 8 0.1405678942929435 0.5655708731000354 9 0.08434073657576609 0.38176033934252385 >10 0.4076468934495361 5.330505478967833 >50 0.056227157717177394 1.6967126193001063 >100 0.11245431543435479 10.441852244609402 >500 0.0 0.0 >1k 0.014056789429294348 22.933898904206433 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 3244 22.933898904206433 No Hit AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC 366 2.5874867444326615 No Hit AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC 244 1.7249911629551078 No Hit TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT 214 1.5129020855425945 No Hit AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA 186 1.3149522799575823 No Hit TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT 138 0.975609756097561 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122 0.8624955814775539 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG 106 0.7493814068575468 No Hit GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC 101 0.7140332272887946 No Hit GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC 72 0.5090137857900319 No Hit AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA 62 0.43831742665252743 No Hit AAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAG 53 0.3746907034287734 No Hit GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA 53 0.3746907034287734 No Hit AAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGA 49 0.34641215977377166 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 48 0.3393425238600212 No Hit AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA 47 0.33227288794627075 No Hit GTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCA 42 0.29692470837751855 No Hit AAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGC 40 0.2827854365500177 No Hit AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG 37 0.26157652880876636 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35 0.24743725698126548 No Hit GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA 33 0.23329798515376457 No Hit AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC 32 0.22622834924001414 No Hit AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG 29 0.2050194414987628 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 29 0.2050194414987628 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 27 0.19088016967126192 No Hit AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC 26 0.18381053375751147 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26 0.18381053375751147 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24 0.1696712619300106 No Hit AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA 23 0.16260162601626016 No Hit AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA 22 0.15553199010250973 No Hit AAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCC 21 0.14846235418875928 No Hit AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG 21 0.14846235418875928 No Hit GGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACG 21 0.14846235418875928 No Hit AAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC 18 0.12725344644750797 No Hit AAAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCG 15 0.10604453870625664 No Hit AAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC 15 0.10604453870625664 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.007069635913750442 2 0.0 0.0 0.014139271827500884 3 0.0 0.0 0.042417815482502653 4 0.0 0.0 0.056557087310003536 5 0.0 0.0 0.06362672322375397 6 0.0 0.0 0.07069635913750442 7 0.0 0.0 0.08483563096500531 8 0.0 0.0 0.10604453870625663 9 0.0 0.0 0.16967126193001061 10 0.0 0.0 0.22622834924001414 11 0.0 0.0 0.26157652880876636 12 0.0 0.0 0.29692470837751855 13 0.0 0.0 0.3181336161187699 14 0.0 0.0 0.33227288794627075 15 0.0 0.0 0.4171085189112761 16 0.0 0.0 0.5302226935312832 17 0.0 0.0 0.721102863202545 18 0.0 0.0 0.9402615765288088 19 0.0 0.0 1.1028632025450689 20 0.0 0.0 1.3078826440438318 21 0.0 0.0 2.036055143160127 22 0.0 0.0 2.8278543655001767 23 0.0 0.0 5.387062566277836 24 0.0 0.0 8.15129020855426 25 0.0 0.0 8.80876634853305 26 0.0 0.0 12.753623188405797 27 0.0 0.0 13.6090491339696 28 0.0 0.0 14.450335807705903 29 0.0 0.0 15.312831389183456 30 0.0 0.0 15.68752209261223 31 0.0 0.0 16.380346412159774 32 0.0 0.0 17.221633085896077 33 0.0 0.0 17.94980558501237 34 0.007069635913750442 0.0 19.172852598091197 35 0.007069635913750442 0.0 20.077765995051255 36 0.007069635913750442 0.0 21.336161187698835 37 0.007069635913750442 0.0 22.615765288087662 38 0.007069635913750442 0.0 24.33368681512902 39 0.007069635913750442 0.0 25.853658536585368 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTCT 30 2.0164734E-6 45.000004 45 TGCCGTC 395 0.0 41.582275 45 ATGCCGT 405 0.0 41.11111 44 AGACGAT 415 0.0 40.120483 25 CCTATCT 410 0.0 40.060974 34 GATAACC 410 0.0 40.060974 29 AACCTAT 410 0.0 40.060974 32 ATAACCT 410 0.0 40.060974 30 TAACCTA 410 0.0 40.060974 31 CTATCTC 410 0.0 40.060974 35 GTGGTAT 45 1.7071216E-8 40.000004 1 ATCTCGT 420 0.0 39.642857 37 TATGCCG 420 0.0 39.642857 43 TCTCGTA 420 0.0 39.642857 38 ACGAGAC 420 0.0 39.642857 22 TATCTCG 420 0.0 39.642857 36 CGTATGC 420 0.0 39.642857 41 TCGTATG 420 0.0 39.642857 40 CGAGACG 420 0.0 39.642857 23 CTCGTAT 420 0.0 39.642857 39 >>END_MODULE