FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_52.34100000005f02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_52.34100000005f02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368074
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC12510.33987730728060117No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA4600.12497486918391411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG1600.035.15529623
CGATAAC1600.035.15529628
TGCCGTC1650.034.0899845
GAGACGA1700.033.08733724
GACGATA1700.033.08733726
ACGAGAC1700.033.08733722
TCGTATG1650.032.72638340
CCACGAG1750.032.14198320
TATCTCG1700.031.76384436
TATGCCG1800.031.24915143
ACGATAA1800.031.24915127
ATGCCGT1800.031.24915144
TCTCGTA1750.030.85630638
GCCCACG1850.030.40870918
CGTATGC1850.030.4045841
AGACGAT1850.030.4045825
CTCGTAT1800.029.99918639
CATGCGG300.00514344929.9991843
GATAGCG300.00514344929.9991845
CCCACGA1900.029.60445819
Produced by FastQC (version 0.11.2)