FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_51.34100000005ef4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_51.34100000005ef4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363374
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC11500.3164783391216763No Hit
ACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGG5350.14723122733051897No Hit
TTCCCAAACAACCCGACTCGTTGACGGCGCCTCGTGGGGCGACAGGGTCCG5000.13759927787898968No Hit
CCCCTAGTAACGGCGAGCGAACCGGGAGCAGCCCAGCTTGAGAATCGGGCG4260.1172345847528992No Hit
AACCGGGAGCAGCCCAGCTTGAGAATCGGGCGGCTGTGCCGTCCGAATTGT4260.1172345847528992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT2050.035.12162844
CGAGACG1950.034.61506723
CGATAAC1900.034.3417928
TATGCCG2100.034.285443
TCTCGTA2100.034.285438
ACGAGAC2100.034.285422
CGAGCCC2150.033.48806415
CTCGTAT2200.032.72697439
CACGAGA2200.032.72697421
TCGTATG2200.032.72697440
ACGATAA2000.032.624727
GAGACGA2100.032.14256324
TATCTCG2100.032.14256336
ATCTCGT2250.031.99970637
CGTATGC2250.031.99970641
TCTCCGA2250.031.99970611
GACGATA2150.031.3950626
GATAACC2150.030.34855729
GTCTAGC300.005135991630.0079841
CTCCGAG2400.029.99972712
Produced by FastQC (version 0.11.2)