##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_50.34100000005ee7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 151189 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.318984846781184 38.0 38.0 38.0 32.0 38.0 2 36.33090370331175 38.0 38.0 38.0 32.0 38.0 3 36.285106720727036 38.0 38.0 38.0 32.0 38.0 4 36.38160183611242 38.0 38.0 38.0 32.0 38.0 5 36.41202071579281 38.0 38.0 38.0 32.0 38.0 6 38.30336201707796 40.0 38.0 40.0 38.0 40.0 7 38.20461806083776 40.0 38.0 40.0 38.0 40.0 8 38.184094080918584 40.0 38.0 40.0 38.0 40.0 9 38.196012937449154 40.0 38.0 40.0 38.0 40.0 10 38.13948766113937 40.0 38.0 40.0 38.0 40.0 11 38.13108757912282 40.0 38.0 40.0 38.0 40.0 12 38.04399129566304 40.0 38.0 40.0 38.0 40.0 13 38.065514025491275 40.0 38.0 40.0 32.0 40.0 14 37.993114578441556 40.0 38.0 40.0 32.0 40.0 15 38.0332100880355 40.0 38.0 40.0 32.0 40.0 16 38.018222225161885 40.0 38.0 40.0 32.0 40.0 17 38.03383182638949 40.0 38.0 40.0 32.0 40.0 18 38.07324606948918 40.0 38.0 40.0 38.0 40.0 19 38.083312939433426 40.0 38.0 40.0 38.0 40.0 20 38.0630667575022 40.0 38.0 40.0 38.0 40.0 21 38.08136835351778 40.0 38.0 40.0 38.0 40.0 22 38.067094828327456 40.0 38.0 40.0 38.0 40.0 23 38.0411670161189 40.0 38.0 40.0 32.0 40.0 24 38.051597669142595 40.0 38.0 40.0 32.0 40.0 25 38.0340831674262 40.0 38.0 40.0 32.0 40.0 26 37.985164264595966 40.0 38.0 40.0 32.0 40.0 27 37.96264939909649 40.0 38.0 40.0 32.0 40.0 28 37.923506339746936 40.0 38.0 40.0 32.0 40.0 29 37.89430447982327 40.0 38.0 40.0 32.0 40.0 30 37.87957457222417 40.0 38.0 40.0 32.0 40.0 31 37.86075045142173 40.0 38.0 40.0 32.0 40.0 32 37.8389829947946 40.0 38.0 40.0 32.0 40.0 33 37.79730668236446 40.0 38.0 40.0 32.0 40.0 34 37.80707591160733 40.0 38.0 40.0 32.0 40.0 35 37.80679811361938 40.0 38.0 40.0 32.0 40.0 36 37.74120471727441 40.0 38.0 40.0 32.0 40.0 37 37.74816289544874 40.0 38.0 40.0 32.0 40.0 38 37.718584023969996 40.0 38.0 40.0 32.0 40.0 39 37.66090125604376 40.0 38.0 40.0 32.0 40.0 40 37.636388890726174 40.0 38.0 40.0 32.0 40.0 41 37.63215577852886 40.0 38.0 40.0 32.0 40.0 42 37.58733770313978 40.0 38.0 40.0 32.0 40.0 43 37.556244171202934 40.0 38.0 40.0 32.0 40.0 44 37.498382818855866 40.0 38.0 40.0 32.0 40.0 45 37.45066109306894 40.0 38.0 40.0 32.0 40.0 46 37.36142179655927 40.0 38.0 40.0 32.0 40.0 47 37.3313468572449 40.0 38.0 40.0 32.0 40.0 48 37.28521254853197 40.0 38.0 40.0 32.0 40.0 49 37.22157035234045 40.0 38.0 40.0 32.0 40.0 50 37.200378334402636 40.0 38.0 40.0 32.0 40.0 51 36.74605295358789 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.16175922599416737 2201 2 -0.11159027485683737 2201 3 -0.16742373223944185 2201 4 0.02022517434804172 2201 5 -0.0964885209123807 2201 6 0.04999055831672905 2201 7 0.2173849478641543 2201 8 -0.2056581617567943 2201 9 -0.22270712591708275 2201 10 -0.18429987672879378 2201 11 -0.042971043572684664 2201 12 -0.07524508986125511 2201 13 -1.042335012770863 2201 14 -0.2153594974650943 2201 15 -0.22498525691243287 2201 16 0.3339014177028119 2201 17 -0.33571267088309753 2201 18 0.007666191574983827 2201 19 -0.23577738238234502 2201 20 -0.05813383507398129 2201 21 -0.07136009064973337 2201 22 0.08641547344722511 2201 23 -0.21899140693058428 2201 24 -0.43080578713077244 2201 25 -0.3524303434481979 2201 26 0.01867683371003892 2201 27 -0.27227919405577694 2201 28 -0.34934599799432675 2201 29 -0.3047740804100769 2201 30 -0.34694985704118864 2201 31 -0.1901540857098638 2201 32 -0.3076716367396841 2201 33 -0.371526323560154 2201 34 -0.4278548575656913 2201 35 -0.41726496262155166 2201 36 -0.1872726279603043 2201 37 -0.7059254534625339 2201 38 -0.8585550831137141 2201 39 -0.5485134286996924 2201 40 -0.9022216154104328 2201 41 -0.4647229929449921 2201 42 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1101 27 0.2858882931734925 1101 28 0.319932600346057 1101 29 0.6604155735667661 1101 30 0.5851630338817699 1101 31 0.7271424494356467 1101 32 0.4479625079104821 1101 33 0.4948028722884956 1101 34 0.3240116334312333 1101 35 0.30087756101296037 1101 36 0.3190029423884937 1101 37 0.07328541956984935 1101 38 0.22872504077967903 1101 39 0.2446354514057063 1101 40 -0.09143641789754753 1101 41 0.19854585110188339 1101 42 -0.15715087864396082 1101 43 0.21745040109662028 1101 44 0.039907029226391444 1101 45 0.22800370173839468 1101 46 0.3381523047422519 1101 47 -0.1662827837744416 1101 48 -0.2151170771914721 1101 49 0.46764519580743524 1101 50 0.17321960211778986 1101 51 0.12523422006781715 2202 1 0.31750801826470365 2202 2 0.28879619857310956 2202 3 0.18909800689099399 2202 4 -0.15330139570026802 2202 5 -0.19619866583992263 2202 6 -0.3931012291228839 2202 7 -0.09623824054163777 2202 8 0.030283867228710903 2202 9 -0.30193417905717723 2202 10 -0.15492789605246315 2202 11 0.05867146850460614 2202 12 0.1863974222160394 2202 13 -0.09933984368873894 2202 14 0.16164533345277476 2202 15 0.1993625691745251 2202 16 -0.13276524896385666 2202 17 0.23008443056617978 2202 18 0.13771450075373082 2202 19 0.058522134525865965 2202 20 0.05201109246225144 2202 21 0.10815681756282203 2202 22 -0.15223186954794699 2202 23 -0.04990928132671968 2202 24 -0.0047188306090362175 2202 25 0.5780044391604946 2202 26 0.03742079506270102 2202 27 -0.43711011193017413 2202 28 -0.3275102492020565 2202 29 -0.31134412871925576 2202 30 0.0499583555191947 2202 31 -0.17933282967121045 2202 32 -0.5550146319087617 2202 33 -0.097903135154354 2202 34 0.09774900716860913 2202 35 0.1641843127407654 2202 36 -0.14147552651103013 2202 37 -0.4539447771340335 2202 38 0.022217863746185174 2202 39 0.042211208981463244 2202 40 -0.4618351419804867 2202 41 -0.2531287900464463 2202 42 -0.14911260113199631 2202 43 -0.4431131289405954 2202 44 -0.14756771824835369 2202 45 -0.1389287809835622 2202 46 -0.12005077872399283 2202 47 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-0.4847730226754621 1213 48 -0.44971688046906877 1213 49 -0.41553837287035833 1213 50 -0.5779598987407866 1213 51 -1.6062063547617242 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 1.0 16 4.0 17 2.0 18 5.0 19 7.0 20 14.0 21 21.0 22 55.0 23 128.0 24 251.0 25 403.0 26 619.0 27 897.0 28 1281.0 29 1800.0 30 2221.0 31 2783.0 32 3376.0 33 4239.0 34 5048.0 35 6443.0 36 8541.0 37 12888.0 38 23721.0 39 76440.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.26582940176143 21.78432708913695 14.09476797660246 25.855075532499157 2 13.95811836918274 22.824562795989102 40.43178030002381 22.78553853480435 3 18.90944447016648 25.5250051260343 30.119254707683762 25.446295696115456 4 13.037985567733102 19.37177969296708 37.16672509243397 30.423509646865842 5 13.461958211245529 37.443861656602 35.38484942687629 13.709330705276177 6 30.240956683356597 39.24822573070792 17.057457883840755 13.45335970209473 7 25.727400802968468 33.84505486510262 21.477091587350934 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30.060387991189835 20.264701797088414 21 19.71638148277983 29.303719185919608 29.56365873178604 21.416240599514516 22 20.131094193360628 31.28005344304149 29.16217449682186 19.42667786677602 23 19.289101720363256 29.917520454530422 29.90495340269464 20.888424422411685 24 21.12322986460655 29.285199320056353 30.39969839075594 19.191872424581153 25 19.856603324315923 30.295854857165534 30.23698814067161 19.610553677846934 26 20.248434058483863 29.925191980792 30.392824779908324 19.43354918081581 27 19.58277387905205 30.875262089173155 29.87320506121477 19.668758970560027 28 19.45710336069423 29.793172783734267 29.567627274471025 21.182096581100478 29 21.051796096276846 29.49619350614132 29.53720178055282 19.914808617029017 30 19.51806750759014 30.314254909612853 30.037768796756247 20.129908786040758 31 20.56432676980468 29.112567713259562 30.533967418264556 19.7891380986712 32 19.669420394340857 30.604078339032604 29.80838553069337 19.91811573593317 33 19.67537320836833 30.705937601280514 29.945961677106137 19.67272751324501 34 19.872477495055858 29.48362645430554 30.276012143740616 20.367883906897987 35 19.82419355905522 29.54712313726528 30.077585009491433 20.55109829418807 36 19.41344939115941 29.309010576166255 31.36736138211113 19.9101786505632 37 19.758712604752994 29.645013856828207 29.62583256718412 20.97044097123468 38 19.75738975719133 29.12182764619119 30.626566747580842 20.49421584903664 39 20.107282937250726 28.43460833790818 30.214499732123368 21.243608992717725 40 20.70270127258777 28.1020980501098 31.063973331216765 20.131227346085666 41 20.38852289864275 27.88911818398286 30.278858110432044 21.44350080694235 42 20.559696803338866 28.546388956868558 30.60341691525177 20.29049732454081 43 19.383685321022032 28.3810330116609 31.500968985838917 20.73431268147815 44 18.96301979641376 29.415499804879985 30.403005509660098 21.21847488904616 45 19.203116628855273 28.457096746456422 31.239045168629993 21.100741456058312 46 20.311662885527387 27.848586868092255 29.983001408832656 21.856748837547705 47 19.92869851642646 27.8776895144488 29.967127238092715 22.22648473103202 48 18.810230903041887 28.334071923221927 29.81103122581669 23.04466594791949 49 20.042463406729325 27.961690334614293 30.123884674149576 21.87196158450681 50 18.902168808577343 28.800375688707515 30.513463281058804 21.783992221656337 51 18.31043674389994 28.700880366698193 30.017792535072456 22.970890354329406 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1015.0 1 574.0 2 133.0 3 180.5 4 228.0 5 226.0 6 224.0 7 267.5 8 311.0 9 282.5 10 254.0 11 482.5 12 711.0 13 1070.5 14 1430.0 15 1513.5 16 1597.0 17 1349.5 18 1102.0 19 959.5 20 817.0 21 952.5 22 1088.0 23 952.0 24 816.0 25 916.0 26 1414.0 27 1812.0 28 2611.0 29 3410.0 30 5053.5 31 6697.0 32 8568.5 33 10440.0 34 11533.5 35 12627.0 36 12744.0 37 12861.0 38 12224.5 39 11588.0 40 10831.5 41 10075.0 42 9532.0 43 8989.0 44 8717.5 45 8446.0 46 8332.0 47 8218.0 48 8139.0 49 8060.0 50 8560.0 51 9060.0 52 7897.0 53 6734.0 54 6164.0 55 5594.0 56 5101.0 57 4608.0 58 4065.0 59 3522.0 60 3102.5 61 2683.0 62 2253.0 63 1823.0 64 1564.0 65 1305.0 66 1077.0 67 849.0 68 659.5 69 470.0 70 369.5 71 269.0 72 211.5 73 154.0 74 114.5 75 64.0 76 53.0 77 35.5 78 18.0 79 10.5 80 3.0 81 2.5 82 2.0 83 1.5 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041008274411498194 2 6.61423780830616E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.001984271342491848 12 0.0 13 0.0 14 0.0 15 0.0 16 0.001984271342491848 17 0.0 18 0.002645695123322464 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.001322847561661232 27 0.0 28 0.0 29 0.0 30 0.003968542684983696 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 6.61423780830616E-4 41 6.61423780830616E-4 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.001322847561661232 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 151189.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.47999663727248 #Duplication Level Percentage of deduplicated Percentage of total 1 91.7346826195882 77.4974567922407 2 6.46962507806995 10.931078096795261 3 1.0102213018864317 2.5603047655880036 4 0.3039577322998433 1.0271339281025493 5 0.13745079554029296 0.5805921372517188 6 0.09896433634164635 0.5016304080811311 7 0.05651810373103038 0.33422544491997247 8 0.019043894500272596 0.12870625146748807 9 0.027033398378047156 0.20554032636643238 >10 0.1213353991222575 1.9501245606922168 >50 0.009511121413066122 0.5832877789595744 >100 0.009326180058495549 1.57730992181578 >500 0.0015533593802761476 1.1836747129042857 >1k 7.766796901380738E-4 0.9389348748148741 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 1431 0.9464974303686116 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 972 0.6429039149673587 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 832 0.5503045856510725 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 478 0.31616056723703445 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 390 0.25795527452394024 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 383 0.2533253080581259 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 6.614237808306159E-4 15 0.0 0.0 6.614237808306159E-4 16 0.0 0.0 0.0013228475616612319 17 0.0 0.0 0.0013228475616612319 18 0.0 0.0 0.0013228475616612319 19 0.0 0.0 0.001984271342491848 20 0.0 0.0 0.00330711890415308 21 0.0 0.0 0.003968542684983696 22 0.0 0.0 0.005952814027475544 23 0.0 0.0 0.011244204274120472 24 0.0 0.0 0.019181289644087864 25 0.0 0.0 0.021826984767410327 26 0.0 0.0 0.03505546038402265 27 0.0 0.0 0.05092963112395743 28 0.0 0.0 0.07209519211053714 29 0.0 0.0 0.10185926224791486 30 0.0 0.0 0.16734021655014583 31 0.0 0.0 0.3750272837309593 32 0.0 0.0 0.6296754393507464 33 0.0 0.0 0.8962292230254847 34 0.0 0.0 1.2051141286733824 35 0.0 0.0 1.5298732050612147 36 0.0 0.0 1.9300345924637374 37 0.0 0.0 2.4849691445806243 38 0.0 0.0 3.1067074985614034 39 0.0 0.0 3.750934261090423 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTAGCT 20 7.0140907E-4 44.999672 3 CGAGACG 225 0.0 38.99971 23 CGATAAC 220 0.0 38.86335 28 AGACGAT 225 0.0 37.999718 25 ACGATAA 225 0.0 37.999718 27 GACGATA 225 0.0 37.999718 26 TATCTCG 235 0.0 37.34015 36 GATAACC 230 0.0 37.173637 29 TAACCTA 230 0.0 37.173637 31 GAGACGA 230 0.0 37.173637 24 CCCACGA 245 0.0 36.734425 19 CTCGTAT 240 0.0 36.562233 39 CGAGCCC 255 0.0 36.176205 15 GCCCACG 250 0.0 35.999737 18 TCTCCGA 250 0.0 35.999737 11 ATCTCGT 245 0.0 35.816063 37 TATGCCG 245 0.0 35.816063 43 TCTCGTA 245 0.0 35.816063 38 TCGTATG 245 0.0 35.816063 40 CCGAGCC 255 0.0 35.293858 14 >>END_MODULE