FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_50.34100000005ee7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_50.34100000005ee7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151189
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC14310.9464974303686116No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9720.6429039149673587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.5503045856510725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.31616056723703445No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.25795527452394024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.2533253080581259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCT207.0140907E-444.9996723
CGAGACG2250.038.9997123
CGATAAC2200.038.8633528
AGACGAT2250.037.99971825
ACGATAA2250.037.99971827
GACGATA2250.037.99971826
TATCTCG2350.037.3401536
GATAACC2300.037.17363729
TAACCTA2300.037.17363731
GAGACGA2300.037.17363724
CCCACGA2450.036.73442519
CTCGTAT2400.036.56223339
CGAGCCC2550.036.17620515
GCCCACG2500.035.99973718
TCTCCGA2500.035.99973711
ATCTCGT2450.035.81606337
TATGCCG2450.035.81606343
TCTCGTA2450.035.81606338
TCGTATG2450.035.81606340
CCGAGCC2550.035.29385814
Produced by FastQC (version 0.11.2)