FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_5.34100000005bb3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_5.34100000005bb3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418934
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAGTACTCTGCGTTGATAC55741.330519843221128No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAGTACTCTGCGTTGATA48401.1553132474327699No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATA46911.1197467858899015No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTAT41300.9858354776647396No Hit
GCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCC41230.9841645700754773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTGCGCAAGCAGTGGTATCAACGC35440.8459566423350695No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGCAAGCAGTGGTATC34280.8182673165701518No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGCAAGCAGTGGTATCAACG31950.7626499639561363No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGCAAGCAGTGGTAT30250.7220707796454812No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAGTACTCTGCGTTGATACC28880.6893687311127767No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAGTACTCTGCGTTGAT28520.680775492082285No Hit
GTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGG27480.6559505793275313No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAGTACTCTGCGTTGATACCAC22010.5253810862808939No Hit
ACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGC21610.5158330429136809No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGCAAGCAGTGGTATCAA20590.4914855323272878No Hit
ATACCACTGCTTGCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC20190.4819374889600748No Hit
CAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTA19880.4745377553504848No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGCAAGCAGTGGTATCAAC19610.46809282607761604No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGCAAGCAGTGGTATCA19100.4559190707844195No Hit
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCA18340.43777778838671483No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGCAAGCAGTGGTATCA18330.43753908730253455No Hit
ATACCACTGCTTGCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG18320.4373003862183542No Hit
CAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACC17950.4284684461036822No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGA17580.4196365059890102No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGCAAGCAGTGGTA17210.4108045658743382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTGCGCAAGCAGTGGTATCAACGCA17070.40746275069581367No Hit
GTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACT16990.405553142022371No Hit
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGT16770.40030171817040394No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTGCAAGCAGTGGTATCAAC16570.3955276964867974No Hit
GATACCACTGCTTGCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG15930.38025082709925667No Hit
ACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAGTACTCTGCGTT15100.3604386371122898No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTGCAAGCAGTGGTATCAACGCAGA15010.35829032735466687No Hit
GATACCACTGCTTGCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT14470.34540046880892933No Hit
CTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAA14460.345161767724749No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC13680.3265430831586837RNA PCR Primer, Index 27 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTGCGCAAGCAGTGGTATCAACG13520.3227238658117985No Hit
ATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTAC13270.3167563387072904No Hit
ACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAGTACTCTGCGT13110.3129371213604052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTGCGCAAGCAGTGGTATCAACGCA12510.29861505630958574No Hit
TATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGG12360.2950345400468809No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTGCAAGCAGTGGTATCAACGC12120.2893057140265531No Hit
GATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTA12110.2890670129423728No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGCAAGCAGTGGTATCAAC11850.28286078475368437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGCAAGCAGTGGTATCAAC11820.28214468150114336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTGCGCAAGCAGTGGTATCAACGC11520.27498364897573363No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAGTACTCTGCGTTGATACCA11250.2685387197028649No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGCAAGCAGTGGTATC11210.26758391536614357No Hit
ATACCACTGCTTGCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT11180.26686781211360266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGCAAGCAGTGGTATCAA11170.26662911102942227No Hit
ACGCAGAGTACTTTTTTTTTTTTGCGCAAGCAGTGGTATCAACGCAGAGTA11110.2651969045243403No Hit
ATACCACTGCTTGCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT11090.2647195023559797No Hit
GGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTG10650.2542166546520454No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAGTACTCTGCGTTGATACCA10580.25254574706278315No Hit
TGATACCACTGCTTGCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACT10540.25159094272606186No Hit
ATACCACTGCTTGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTG10500.25063613838934057No Hit
GCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTT10480.2501587362209799No Hit
ACGCAGAGTACTTTTTTTTTTTTTGCGCAAGCAGTGGTATCAACGCAGAGT9440.22533382346622619No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTG9400.22437901912950486No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCAAGCAGTGGTATCAA9380.22390161696114425No Hit
CTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTG9290.22175330720352132No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAGTAC9260.22103720395098034No Hit
TGATACCACTGCTTGCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC9240.22055980178261972No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAGTACT9220.22008239961425904No Hit
GTTGATACCACTGCTTGCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACC9110.2174566876882755No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT9100.21721798660409514No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAGTACTCT9100.21721798660409514No Hit
GCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGT9060.21626318226737384No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTGCAAGCAGTGGTATCAACGCAG8990.2145922746781116No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA8900.21244396492048867No Hit
GATACCACTGCTTGCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC8880.21196656275212802No Hit
GATACCACTGCTTGCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC8840.2110117584154067No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGCAAGCAGTGGT8820.21053435624704608No Hit
GTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACT8740.20862474757360347No Hit
GTTGATACCACTGCTTGCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCA8430.20122501396401343No Hit
TACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACT8390.20027020962729214No Hit
ACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAGTACTCTGCG8220.19621229119622663No Hit
TGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGT8220.19621229119622663No Hit
GATACCACTGCTTGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT8130.1940639814386037No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAGTACTC7970.1902447640917185No Hit
ACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCA7880.18809645433409558No Hit
ACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAGTACTCTGCGTTG7750.18499334023975136No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTGCGCAAGCAGTGGTATCAA7650.18260632939794813No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGCAAGCAGTGGTA7600.1814128239770465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTGCGCAAGCAGTGGTATCAACG7350.17544529687253838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTGCGCAAGCAGTGGTATCAACGCAG7220.1723421827781942No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA7160.17090997627311225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGCAAGCAGTGGTATCAA7150.1706712751889319No Hit
GTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAA7030.16780686217876803No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGC6970.16637465567368606No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC6960.16613595458950575No Hit
ACCACTGCTTGCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT6940.1656585524211451No Hit
ATACCACTGCTTGCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC6820.1627941394109812No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCAAGCAGTGGTATCAACG6770.16160063399007957No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACC6760.16136193290589926No Hit
ACCACTGCTTGCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT6710.16016842748499766No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGCAAGCAGTGGTAT6670.15921362314827633No Hit
CTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCG6650.1587362209799157No Hit
ATACCACTGCTTGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT6640.15849751989573535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTGCGCAAGCAGTGGTATCAACGC6460.15420090038048953No Hit
GCGTTGATACCACTGCTTGAAAAAAAGTACTCTGCGTTGATACCACTGCTT6360.15181388953868627No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAGTA6330.1510977862861453No Hit
GCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGT6110.14584636243417817No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC6090.14536896026581753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTGCGCAAGCAGTGGTATCAACGCAG6010.14345935159237494No Hit
ACGCAGAGTACTTTTTTTTTTTTTTGCGCAAGCAGTGGTATCAACGCAGAG6000.1432206505081946No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAGTACTCTGCGTTGAT5930.14154974291893233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTGCAAGCAGTGGTATCAACGCAG5880.14035623749803072No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGCAAGCAGTGGT5840.13940143316130943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGCAAGCAGTGGTATCA5590.13343390605680133No Hit
ACGCAGAGTACTTTTTTTTTTTGCGCAAGCAGTGGTATCAACGCAGAGTAC5540.13224040063589967No Hit
TTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGA5510.13152429738335872No Hit
CCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCT5490.13104689521499804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG5380.1284211832890145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTGCAAGCAGTGGTATCAACGCAGAG5330.12722767786811287No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAA5300.12651157461557191No Hit
TGATACCACTGCTTGCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA5300.12651157461557191No Hit
TGATACCACTGCTTGCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTG5270.12579547136303093No Hit
ACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAGTACTCTGC5220.12460196594212931No Hit
GTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATAC5190.12388586268958833No Hit
CCACTGCTTGCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTG5190.12388586268958833No Hit
CCACTGCTTGCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT5150.12293105835286704No Hit
GTTGATACCACTGCTTGCGCAAAAAAAAAAAGTACTCTGCGTTGATACCAC5040.12030534642688347No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGCAAGCAGTGGT5000.11935054209016219No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAGTACTC4990.11911184100598185No Hit
ACGCAGAGTACTTTTTTTTTTTGCAAGCAGTGGTATCAACGCAGAGTACTT4980.11887313992180151No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTT4970.1186344388376212No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTGCGCAAGCAGTGGTATCAACG4960.11839573775344087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC4960.11839573775344087No Hit
GATACCACTGCTTGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT4910.11720223233253925No Hit
TGATACCACTGCTTGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT4900.11696353124835894No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTCAAGCAGTGGTATCAACGCAGA4890.1167248301641786No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAGTACT4780.11409911823819503No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGCAAGCAGTGGTAT4770.11386041715401472No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGCAAGCAGTGGTA4760.11362171606983439No Hit
GATACCACTGCTTGCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA4750.11338301498565408No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGCAAGCAGTGG4730.11290561281729342No Hit
GTATCAACGCAGAGTACTTTTTTTCAAGCAGTGGTATCAACGCAGAGTACT4720.11266691173311309No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAGTACTCTG4660.11123470522803114No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC4650.11099600414385082No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG4650.11099600414385082No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGCAAGCAGTGG4640.1107573030596705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCAAGCAGTGGTATCAACGCA4540.10837029221786726No Hit
TTGATACCACTGCTTGCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCAC4530.10813159113368692No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTGCGCAAGCAGTGGTATCAACGCAG4480.1069380857127853No Hit
CTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCT4430.10574458029188369No Hit
GTACTTTTTTTTTTTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTT4430.10574458029188369No Hit
GTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTTT4380.10455107487098206No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA4360.10407367270262143No Hit
GTGGTATCAACGCAGAGTACTTTTTTTCAAGCAGTGGTATCAACGCAGAGT4360.10407367270262143No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCAAGCAGTGGTATCA4350.10383497161844109No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAGTACTCTGCGTTGATACCACT4310.1028801672817198No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGCAAGCAGTGGTAT4230.1009705586082772No Hit
ATACCACTGCTTGCGCAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT4230.1009705586082772No Hit
AAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGA4200.10025445535573622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT207.0301676E-444.99737545
TGCCGTC1700.039.70356445
GTATGCC1750.038.56917642
ATCTCGT1750.038.56917637
CCACGAG1750.038.56917620
TATGCCG1750.038.56917643
CACGAGA1750.038.56917621
CCCACGA1750.038.56917619
TCTCGTA1750.038.56917638
ACGAGAC1750.038.56917622
ATGCCGT1750.038.56917644
CGTATGC1750.038.56917641
TCGTATG1750.038.56917640
CTCGTAT1750.038.56917639
GAGACGA1650.038.18414724
CGAGACG1650.038.17959223
AGCCCAC1800.037.5022917
TATCTCG1750.037.28353536
CGAATTG1450.037.24365228
AGACGAA1450.037.24365225
Produced by FastQC (version 0.11.2)