FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_49.34100000005ecd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_49.34100000005ecd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229348
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC6560.28602821912552107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATA750.039.006826
CGATAAC850.037.06529228
TATGCCG800.036.56090543
TCTCGTA800.036.56090538
CGAGACG800.036.56090523
TCGATCG250.002104050535.99843241
ATGCCGT900.034.99847444
ACGATAA850.034.4177727
CTCGTAT850.034.41026739
TATCTCG850.034.41026736
ACGAGAC850.034.41026722
GAGACGA900.032.50567224
GCCCACG900.032.49858518
TCCGAGC900.032.49858513
CCCACGA900.032.49858519
GTATGCC900.032.49858542
CCGAGCC900.032.49858514
CGAGCCC900.032.49858515
TGCCGTC900.032.49858545
ATAACCT1050.032.14846430
Produced by FastQC (version 0.11.2)