FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_48.34100000005eb0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_48.34100000005eb0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5417
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC414376.48144729555104RNA PCR Primer, Index 46 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT2013.710540889791397RNA PCR Primer, Index 18 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC651.199926158390253No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC561.0337825364592947No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT500.9230201218386561No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC300.5538120731031937No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG210.38766845117223553No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC200.3692080487354624No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA200.3692080487354624No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC170.31382684142514305No Hit
CGGAGATGTGTATAAGAGACAGGCGTCAGATGTGTATAAGAGACAGCTTAT170.31382684142514305No Hit
CTTATACACATCTGACGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC110.20306442680450434No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC110.20306442680450434No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC100.1846040243677312RNA PCR Primer, Index 27 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT80.14768321949418498No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTACGAATCTCGTATGCCGTC80.14768321949418498No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC80.14768321949418498No Hit
CGGAGATGTGTATAAGAGACAGCGTCAGATGTGTATAAGAGACAGCTTATA70.12922281705741184No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC70.12922281705741184TruSeq Adapter, Index 12 (95% over 21bp)
GTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC70.12922281705741184No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC70.12922281705741184No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA70.12922281705741184No Hit
CTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA70.12922281705741184No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGCC70.12922281705741184No Hit
CGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGAC60.11076241462063872No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCATTCCGA60.11076241462063872No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGAC60.11076241462063872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA206.427366E-445.01
CTTATAC4750.042.6315771
TGCCGTC4850.040.82474545
AATCTCG4950.040.45454436
TTCCGAA4900.040.40816531
CGAATCT4900.040.40816534
GAATCTC4900.040.40816535
TCCGAAT4900.040.40816532
CCGAATC4900.040.40816533
ACCATTC4850.040.36082527
CATTCCG4850.040.36082529
CCATTCC4850.040.36082528
AGACCAT4850.040.36082525
ATTCCGA4850.040.36082530
GACCATT4850.040.36082526
GCCGTCT451.3917088E-840.045
GAGACCA4900.039.9489824
CGAGACC5000.039.623
GTATGCC5150.039.3203942
ATCTCGT5150.039.3203937
Produced by FastQC (version 0.11.2)