##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_47.34100000005ea4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57004 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1543926741983 38.0 38.0 38.0 32.0 38.0 2 36.33388884990527 38.0 38.0 38.0 32.0 38.0 3 36.23510630832924 38.0 38.0 38.0 32.0 38.0 4 36.314960353659394 38.0 38.0 38.0 32.0 38.0 5 36.408111711458844 38.0 38.0 38.0 32.0 38.0 6 38.30868009262508 40.0 38.0 40.0 38.0 40.0 7 38.12292119851239 40.0 38.0 40.0 38.0 40.0 8 38.08201178864641 40.0 38.0 40.0 38.0 40.0 9 38.19668795172269 40.0 38.0 40.0 38.0 40.0 10 38.07387200898182 40.0 38.0 40.0 32.0 40.0 11 38.092660164199 40.0 38.0 40.0 38.0 40.0 12 37.880341730404886 40.0 38.0 40.0 32.0 40.0 13 38.01885832573153 40.0 38.0 40.0 32.0 40.0 14 37.87143007508245 40.0 38.0 40.0 32.0 40.0 15 37.855097887867515 40.0 38.0 40.0 32.0 40.0 16 37.71673917619816 40.0 38.0 40.0 32.0 40.0 17 37.52320889762122 40.0 38.0 40.0 32.0 40.0 18 37.54925970107361 40.0 38.0 40.0 32.0 40.0 19 37.55639955090871 40.0 38.0 40.0 32.0 40.0 20 37.65542768928496 40.0 38.0 40.0 32.0 40.0 21 37.754596168689915 40.0 38.0 40.0 32.0 40.0 22 37.736790400673634 40.0 38.0 40.0 32.0 40.0 23 37.79508455546979 40.0 38.0 40.0 32.0 40.0 24 37.77315626973546 40.0 38.0 40.0 32.0 40.0 25 37.55111921970388 40.0 38.0 40.0 32.0 40.0 26 37.545733632727526 40.0 38.0 40.0 32.0 40.0 27 37.62314925268402 40.0 38.0 40.0 32.0 40.0 28 37.64986667602273 40.0 38.0 40.0 32.0 40.0 29 37.57939793698688 40.0 38.0 40.0 32.0 40.0 30 37.61551820924847 40.0 38.0 40.0 32.0 40.0 31 37.55736439548102 40.0 38.0 40.0 32.0 40.0 32 37.57127569995088 40.0 38.0 40.0 32.0 40.0 33 37.5407339835801 40.0 38.0 40.0 32.0 40.0 34 37.54589151638481 40.0 38.0 40.0 32.0 40.0 35 37.50622763314855 40.0 38.0 40.0 32.0 40.0 36 37.347063363974456 40.0 38.0 40.0 32.0 40.0 37 37.360922040558556 40.0 38.0 40.0 32.0 40.0 38 37.43937267560171 40.0 38.0 40.0 32.0 40.0 39 37.21103782190723 40.0 38.0 40.0 32.0 40.0 40 37.25384183566066 40.0 38.0 40.0 32.0 40.0 41 37.35951863027156 40.0 38.0 40.0 32.0 40.0 42 37.35613290295418 40.0 38.0 40.0 32.0 40.0 43 37.52810329099712 40.0 38.0 40.0 32.0 40.0 44 37.368693425022805 40.0 38.0 40.0 32.0 40.0 45 37.47430004911936 40.0 38.0 40.0 32.0 40.0 46 37.1836537786822 40.0 38.0 40.0 32.0 40.0 47 37.168865342782965 40.0 38.0 40.0 32.0 40.0 48 37.17472458073118 40.0 38.0 40.0 32.0 40.0 49 37.03245386288682 40.0 38.0 40.0 32.0 40.0 50 36.94689846326573 38.0 38.0 40.0 32.0 40.0 51 36.30294365307698 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.027980548641103553 2201 2 0.5707678291216638 2201 3 0.4258959404182221 2201 4 0.3019159638975353 2201 5 0.4110397736736715 2201 6 0.37046580699340126 2201 7 0.7131971975880873 2201 8 0.766132616787516 2201 9 1.0520414786126224 2201 10 0.3423623110436651 2201 11 0.13349260216459413 2201 12 0.5008717294995293 2201 13 0.7148285168680673 2201 14 0.8387600382587479 2201 15 0.9161948994821074 2201 16 0.9221136791971247 2201 17 0.7940634305423018 2201 18 0.827888661433434 2201 19 0.5422176619534085 2201 20 0.7346369288596293 2201 21 1.183874264822201 2201 22 1.1082785835153217 2201 23 0.5908495558947706 2201 24 0.5495281171265844 2201 25 1.3454890599365825 2201 26 1.0372043731132266 2201 27 0.6063013733673444 2201 28 0.2353095003480874 2201 29 1.0037625424758332 2201 30 0.4004019376986676 2201 31 1.1175401575293762 2201 32 0.8310763242937895 2201 33 1.326101897110739 2201 34 0.6837171860660689 2201 35 1.0386021305082664 2201 36 0.11246897258327948 2201 37 1.087589697968241 2201 38 0.6198385171648937 2201 39 0.9659789385324657 2201 40 0.818434899964295 2201 41 0.9301829130347414 2201 42 0.5374845393860781 2201 43 0.8643914539961841 2201 44 0.3125232656417154 2201 45 0.8082301658354467 2201 46 0.493886804918624 2201 47 1.2735732790252712 2201 48 0.5385364721064576 2201 49 0.6692155669261055 2201 50 0.43187809822715906 2201 51 1.466629355106619 1101 1 -0.03836898585880988 1101 2 -0.23275520610868483 1101 3 0.49590497384188836 1101 4 0.0616268943401721 1101 5 -0.24659346932542547 1101 6 0.1072920641448647 1101 7 -0.029952456130672545 1101 8 0.1711311112169014 1101 9 0.27667261381286323 1101 10 -0.4182519617657334 1101 11 0.049632319117314694 1101 12 -0.49521378691119367 1101 13 0.3890230365910412 1101 14 -0.4739469777003009 1101 15 0.51782091574227 1101 16 0.23828350756207328 1101 17 -0.3388784544321055 1101 18 -0.03028658698571718 1101 19 0.38247662009854366 1101 20 0.44345957264162195 1101 21 0.7510227140844705 1101 22 0.21803468107629698 1101 23 -0.1456755871344413 1101 24 0.5779834016794325 1101 25 0.5543117037185752 1101 26 0.11925797142698258 1101 27 -0.21860076454292 1101 28 -0.34659354090454997 1101 29 -0.8774479362956242 1101 30 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0.9660967778112308 2202 16 1.0594840184177556 2202 17 0.9071836996760254 2202 18 0.13318723166052138 2202 19 0.7456098682892645 2202 20 0.7912671503342565 2202 21 0.4287579205553129 2202 22 0.7122875546395164 2202 23 0.4907654516054478 2202 24 0.9343052407598904 2202 25 0.4423491664686807 2202 26 0.3333916453307779 2202 27 0.7796963831795125 2202 28 0.5303753820413846 2202 29 0.6691676448027124 2202 30 0.23737979023581346 2202 31 0.4008314387180505 2202 32 0.10469561221081136 2202 33 0.4574447622635276 2202 34 0.06849430969937487 2202 35 0.741293467766468 2202 36 1.2648379100657579 2202 37 1.2915425995662204 2202 38 0.44532211682847134 2202 39 1.2815101972933292 2202 40 1.1022868764150928 2202 41 0.6900651670297009 2202 42 1.1105150972778617 2202 43 0.3383191243073753 2202 44 0.9061593744165961 2202 45 0.5528334739374969 2202 46 -0.18833915527201128 2202 47 -0.11807236156906242 2202 48 -0.04304468853273136 2202 49 0.6055766546749126 2202 50 -0.6710094823727886 2202 51 0.5381180010275628 1102 1 -0.06603520629611381 1102 2 -0.054709690713778514 1102 3 -0.18459480304343145 1102 4 0.2713546944294194 1102 5 0.5106577578053191 1102 6 0.4706715078455943 1102 7 0.3541022813377168 1102 8 0.17812203788654557 1102 9 0.0014978406461239047 1102 10 0.33766057727933685 1102 11 0.6249707097167487 1102 12 0.48500337804492943 1102 13 0.7074820578613057 1102 14 0.6138114634717908 1102 15 0.2326803534932722 1102 16 0.1557310756120529 1102 17 0.09247659539683895 1102 18 -0.09365115637437782 1102 19 0.03545891966719239 1102 20 0.6573521801085249 1102 21 0.7147293926505895 1102 22 -0.19160387314057203 1102 23 -0.38916807560684674 1102 24 -0.7673981244249859 1102 25 0.2862766003421058 1102 26 -0.2838358854821337 1102 27 -0.16698690168407637 1102 28 -0.1365682545740441 1102 29 -0.05192361679539914 1102 30 0.21923825859199297 1102 31 0.1817482092484468 1102 32 0.5125338617607298 1102 33 0.640383413419876 1102 34 0.6438993782494364 1102 35 1.3449494710901106 1102 36 0.840796918513341 1102 37 0.046008739989986225 1102 38 0.05313266938352257 1102 39 0.2779095879594422 1102 40 0.7333629046660306 1102 41 0.1304473855589876 1102 42 0.3708668491127938 1102 43 -0.031435757287972876 1102 44 0.7622734860354825 1102 45 -0.13446745391183157 1102 46 0.7629141337461292 1102 47 0.3890596146115186 1102 48 0.1322772890327002 1102 49 -0.38749909660607784 1102 50 0.5356100994025894 1102 51 0.650290830275587 2203 1 0.29541057683641725 2203 2 -0.21133619673786797 2203 3 0.00817628129392034 2203 4 -0.19852482364287027 2203 5 0.18535655298604325 2203 6 0.03693656808655987 2203 7 0.19747577531362026 2203 8 0.8769172186094352 2203 9 -0.2033214364623106 2203 10 0.5304316616166886 2203 11 0.14304299887337635 2203 12 0.21994313708107427 2203 13 0.5990483229215471 2203 14 0.02345001768865984 2203 15 0.051957461927003123 2203 16 -0.1575219364361402 2203 17 0.502115149613708 2203 18 0.9063488436256222 2203 19 0.27642277508887503 2203 20 0.8501232644458767 2203 21 0.1725607179517965 2203 22 0.4469503437268969 2203 23 0.5385427677666428 2203 24 0.003686212924407073 2203 25 -0.32818123098319063 2203 26 0.8005014639154595 2203 27 -0.058553166744317764 2203 28 0.30921487795608016 2203 29 0.5866306000720698 2203 30 0.2553828369052482 2203 31 0.808254233344833 2203 32 0.1149435003149506 2203 33 0.1946002808897589 2203 34 0.06558612523738816 2203 35 -0.04059269758459294 2203 36 -0.038721153775810535 2203 37 0.021912354447813698 2203 38 -0.561325161941781 2203 39 0.10923488916426294 2203 40 -0.652179264008673 2203 41 0.08225461447464966 2203 42 0.768457210558573 2203 43 0.39025098969997885 2203 44 -0.29796747781993815 2203 45 -0.0019373334299075395 2203 46 0.678576784348536 2203 47 1.2565294941295946 2203 48 0.7467351203580037 2203 49 0.6847900600204326 2203 50 0.969345039161631 2203 51 0.09607396280570413 1103 1 0.4909022208317566 1103 2 0.430327231167297 1103 3 0.21752982449899605 1103 4 -0.12714252023569372 1103 5 0.25997761789081864 1103 6 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6 -1.0571418733387148 1213 7 -0.655510638426442 1213 8 -0.49619224050361055 1213 9 -0.7707400470749732 1213 10 -1.2500092509698248 1213 11 -0.4887180880274187 1213 12 -1.0608822922960073 1213 13 -0.22471481473546362 1213 14 -0.6511206052285416 1213 15 -1.3474273112080866 1213 16 -1.3153019928319338 1213 17 -1.8206226099351284 1213 18 -1.6599162166153434 1213 19 -1.2421622031218362 1213 20 -1.2544653025882155 1213 21 -1.1487671466982974 1213 22 -1.016007872115189 1213 23 -1.3357202417181 1213 24 -1.3085414210156046 1213 25 -1.3351025332133943 1213 26 -1.0027541115780068 1213 27 -1.7468382218583898 1213 28 -0.3752250092523255 1213 29 -1.628826974908705 1213 30 -1.7744813004954167 1213 31 -2.12765963489408 1213 32 -1.1079480659501115 1213 33 -1.5353407598690225 1213 34 -1.057331075480377 1213 35 -0.7579728565181938 1213 36 -0.3299285364981941 1213 37 -1.331714920609862 1213 38 -1.1295128061355726 1213 39 -2.324628222858294 1213 40 -2.9233917736215886 1213 41 -1.7882913993679566 1213 42 -2.4726348171266324 1213 43 -0.957352188972159 1213 44 -0.443314824654081 1213 45 -0.7867361029761781 1213 46 -2.3789725619729154 1213 47 -1.694252356677893 1213 48 -1.9575714676963472 1213 49 -1.4529951232659073 1213 50 -1.73496154889272 1213 51 -2.3745745888477146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 3.0 17 1.0 18 0.0 19 1.0 20 6.0 21 11.0 22 29.0 23 51.0 24 112.0 25 201.0 26 297.0 27 420.0 28 582.0 29 826.0 30 941.0 31 1235.0 32 1421.0 33 1748.0 34 2108.0 35 2659.0 36 3459.0 37 5203.0 38 8897.0 39 26793.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.521595675599784 22.755752119201812 16.572772424928484 40.14987978026993 2 7.401494684397039 23.77986737307463 53.94021262411846 14.87842531840988 3 12.737702617360187 24.607045119640727 48.34222159848432 14.31303066451477 4 12.014946319556524 43.19170584520384 24.917549645638903 19.87579818960073 5 8.676584099361447 29.60844852992772 51.65251561293944 10.062451757771385 6 23.256262718405726 55.4890884850186 12.320188056978457 8.93446073959722 7 13.31310083502912 25.964844572310714 20.993263630622412 39.72879096203775 8 16.661988632376676 53.6471124833345 16.87600870114378 12.814890183145042 9 14.725282436320258 15.234018665356816 13.969195144200409 56.07150375412252 10 9.14672654550558 46.15816433934461 29.257595958178374 15.437513156971441 11 24.018455492789727 17.830953299884214 39.64597733412863 18.50461387319743 12 13.734123921128342 18.07066170795032 28.754122517718056 39.44109185320329 13 26.75250859588801 13.120131920566976 43.056627605080344 17.070731878464667 14 15.660304540032277 17.97944003929549 21.03361167637359 45.32664374429864 15 18.791663742895235 18.863588520103853 23.191354992632096 39.153392744368816 16 39.90842587232251 22.77599424591688 21.298879006368086 16.016700875392523 17 15.733983580099641 47.62648235211564 25.019296891446213 11.620237176338502 18 41.299580723821556 19.10776625791625 23.672438292721434 15.92021472554076 19 14.63756929338292 30.769770542418073 17.347905410146655 37.244754754052344 20 14.623535190512946 20.56697775594695 22.163356957406496 42.646130096133604 21 9.915093677636658 24.550908708160833 17.419830187355274 48.11416742684724 22 15.067363693775876 47.765069117956635 17.90225247351063 19.265314714756858 23 11.992140902392814 18.78464669146025 19.521437092133887 49.701775314013055 24 36.25359623886043 28.606764437583326 21.46866886534278 13.670970458213457 25 21.17921549364957 43.079433022244054 18.333801136762332 17.40755034734405 26 36.82086907706612 24.361875690753116 23.065452695472167 15.751802536708595 27 19.640726966528664 44.863518349589505 22.97733492386499 12.51841976001684 28 11.78864641077819 19.874043926741983 25.294716160269452 43.042593502210366 29 13.006104834748438 23.245737141253244 24.201810399270226 39.54634762472809 30 13.339882811129433 42.61604855969966 24.786849584225116 19.257219044945792 31 13.9727036699179 21.700231562697354 49.22637007929268 15.100694688092064 32 12.576310434355484 21.709002876991086 51.12097396673918 14.593712721914251 33 12.021963370991509 22.393165391902322 27.903305031225877 37.68156620588029 34 11.755315416462004 20.4336537786822 25.278927794540735 42.53210301031506 35 34.309872991369026 21.749350922742263 25.501719177601572 18.43905690828714 36 15.277875236825485 43.44958248543962 24.121114307767876 17.15142796996702 37 11.481650410497508 45.121394989825276 28.06820573994807 15.32874885972914 38 12.078099782471407 20.40032278436601 47.96505508385376 19.556522349308818 39 10.490491895305592 18.04434776506912 32.42754894393376 39.03761139569153 40 10.34137955231212 16.926882324047433 48.9491965476107 23.782541576029754 41 11.064135850115782 16.397094940705912 30.94168830257526 41.59708090660305 42 34.44670549435128 17.747877341940914 29.204968072415973 18.60044909129184 43 10.539611255350502 21.36516735667672 49.3807452108624 18.714476177110377 44 9.487053540102448 43.461862325450845 31.34516876008701 15.705915374359694 45 9.444951231492526 25.105255771524803 48.35450143849555 17.095291558487126 46 33.292400533295904 19.770542418075923 25.50347344046032 21.433583608167847 47 11.497438776226229 22.893130306645148 23.419409164269176 42.190021752859444 48 9.164269174093045 18.728510279980352 19.95123149252684 52.155989053399765 49 34.15725212265806 22.807171426566555 21.273945688021893 21.76163076275349 50 9.98350992912778 18.910953617290016 47.98435197529998 23.121184478282224 51 9.006543515253583 20.73399645632686 21.49360559970528 48.76585442871428 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 209.0 1 120.5 2 32.0 3 34.0 4 36.0 5 36.0 6 36.0 7 52.5 8 69.0 9 58.0 10 47.0 11 76.5 12 106.0 13 143.5 14 181.0 15 200.5 16 220.0 17 177.5 18 135.0 19 104.5 20 74.0 21 95.5 22 117.0 23 78.5 24 40.0 25 56.5 26 88.0 27 103.0 28 108.0 29 113.0 30 263.5 31 414.0 32 716.5 33 1019.0 34 2047.5 35 3076.0 36 3850.0 37 4624.0 38 6417.0 39 8210.0 40 8254.5 41 8299.0 42 6794.5 43 5290.0 44 4438.0 45 3586.0 46 2607.5 47 1629.0 48 1240.0 49 851.0 50 1663.5 51 2476.0 52 8638.0 53 14800.0 54 7608.5 55 417.0 56 318.5 57 220.0 58 192.5 59 165.0 60 134.5 61 104.0 62 95.5 63 87.0 64 72.0 65 57.0 66 47.5 67 38.0 68 33.0 69 28.0 70 19.5 71 11.0 72 8.5 73 6.0 74 4.0 75 1.5 76 1.0 77 1.5 78 2.0 79 1.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043856571468668865 2 0.0035085257174935097 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0035085257174935097 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0017542628587467548 17 0.0 18 0.0017542628587467548 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0017542628587467548 27 0.0 28 0.0 29 0.0 30 0.0035085257174935097 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0017542628587467548 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 57004.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.69758003434238 #Duplication Level Percentage of deduplicated Percentage of total 1 87.05290243243672 38.91054073695639 2 8.622972842369068 7.708520375115044 3 1.6302577040275712 2.1860572260712687 4 0.6618983886359426 1.1834102480262922 5 0.3388144765780999 0.757209359182172 6 0.22052422354625978 0.5914139478882096 7 0.2063046245763101 0.6454922227918206 8 0.11055428998439967 0.3953207379774081 9 0.12889124850623984 0.5185014206250572 >10 0.7990331706130008 7.128826072651982 >50 0.12963685847066783 4.082456116114871 >100 0.08249618181483337 8.356304733754568 >500 0.00785677922046032 1.9402640745532254 >1k 0.00392838961023016 1.808571942796219 >5k 0.0 0.0 >10k+ 0.00392838961023016 23.787110785495514 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC 13547 23.764998947442283 RNA PCR Primer, Index 46 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT 1030 1.8068907445091573 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG 588 1.0315065609430918 No Hit TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 517 0.9069538979720722 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACATC 491 0.8613430636446565 No Hit TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC 452 0.792926812153533 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACA 435 0.7631043435548382 No Hit TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT 432 0.757841554978598 RNA PCR Primer, Index 18 (95% over 21bp) AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC 359 0.6297803662900849 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC 335 0.5876780576801628 No Hit AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC 220 0.385937828924286 No Hit CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG 199 0.34909830889060417 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 199 0.34909830889060417 No Hit GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC 176 0.3087502631394288 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG 170 0.2982246859869483 No Hit TCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATC 168 0.2947161602694548 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGACTGTCTCTTATACACATC 158 0.2771735316819872 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACATC 142 0.24910532594203916 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA 134 0.23507122307206513 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGA 132 0.2315626973545716 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 126 0.2210371202020911 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTATAC 112 0.1964774401796365 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACA 109 0.19121465160339626 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGACTGTCTCTTATACACATCTC 105 0.18419760016840922 No Hit AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA 105 0.18419760016840922 No Hit TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 100 0.17542628587467546 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAACTGTCTCTTATA 99 0.1736720230159287 No Hit GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA 97 0.1701634972984352 No Hit TCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTC 93 0.16314644586344818 No Hit GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC 92 0.16139218300470143 No Hit TTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATAC 91 0.15963792014595468 No Hit TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC 90 0.1578836572872079 No Hit ACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTATACACATC 87 0.15262086871096764 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 86 0.1508666058522209 No Hit AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC 82 0.14384955441723388 No Hit ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAACTGTCTCTTATACACAT 77 0.1350782401235001 No Hit GTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCA 71 0.12455266297101958 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 70 0.12279840011227283 No Hit AAAGTACTCTGCGTTGATACCACTGCTTAGCAACTGTCTCTTATACACATC 68 0.11928987439477931 No Hit TTGCTAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 68 0.11928987439477931 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68 0.11928987439477931 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 67 0.11753561153603256 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTAT 66 0.1157813486772858 No Hit AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA 66 0.1157813486772858 No Hit TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACAC 61 0.10701003438355203 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACATC 61 0.10701003438355203 No Hit TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 59 0.10350150866605852 No Hit TTGCTAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC 59 0.10350150866605852 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACAC 58 0.10174724580731176 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCCAAACTGTCTCTTATACACATC 58 0.10174724580731176 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGA 58 0.10174724580731176 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0017542628587467546 2 0.0 0.0 0.0070170514349870185 3 0.0 0.0 0.012279840011227281 4 0.0 0.0 0.01578836572872079 5 0.0 0.0 0.0192968914462143 6 0.0 0.0 0.0192968914462143 7 0.0 0.0 0.02280541716370781 8 0.0 0.0 0.024559680022454563 9 0.0 0.0 0.0385937828924286 10 0.0 0.0 0.06490772577362992 11 0.0 0.0 0.07192477720861694 12 0.0 0.0 0.0736790400673637 13 0.0 0.0 0.0771875657848572 14 0.0 0.0 0.08946740579608449 15 0.0 0.0 0.10525577152480528 16 0.0 0.0 0.1385867658409936 17 0.0 0.0 0.15963792014595468 18 0.0 0.0 0.18770612588590274 19 0.0017542628587467546 0.0 0.2087572801908638 20 0.0017542628587467546 0.0 0.22103712020209107 21 0.0017542628587467546 0.0 0.3473440460318574 22 0.0017542628587467546 0.0 0.48593081187285103 23 0.0017542628587467546 0.0 0.857834537927163 24 0.0017542628587467546 0.0 1.52971721282717 25 0.0017542628587467546 0.0 1.6419900357869623 26 0.0017542628587467546 0.0 2.4700021051154306 27 0.0017542628587467546 0.0 2.796294996842327 28 0.0017542628587467546 0.0 3.780436460599256 29 0.0017542628587467546 0.0 4.917198793067153 30 0.0017542628587467546 0.0 5.536453582204757 31 0.0017542628587467546 0.0 7.7696302013893765 32 0.0017542628587467546 0.0 9.371272191425163 33 0.0017542628587467546 0.0 10.69223212406147 34 0.0017542628587467546 0.0 15.814679671601994 35 0.0017542628587467546 0.0 17.819802119149532 36 0.0017542628587467546 0.0 21.84934390569083 37 0.0017542628587467546 0.0 23.95095081046944 38 0.0017542628587467546 0.0 25.822749280752227 39 0.0017542628587467546 0.0 27.71209037962248 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 1500 0.0 42.3 45 ATGCCGT 1630 0.0 39.06442 44 CCGAATC 1635 0.0 38.394497 33 CGAATCT 1630 0.0 38.374233 34 AATCTCG 1625 0.0 38.215385 36 CATTCCG 1650 0.0 38.18182 29 ATTCCGA 1650 0.0 38.18182 30 TCCGAAT 1650 0.0 38.18182 32 TCTCGTA 1645 0.0 38.161095 38 CTCGTAT 1645 0.0 38.161095 39 GTATGCC 1675 0.0 38.149254 42 TATGCCG 1675 0.0 38.149254 43 CGTATGC 1670 0.0 38.128742 41 GAATCTC 1630 0.0 38.09816 35 TCGTATG 1660 0.0 38.08735 40 TTCCGAA 1655 0.0 38.066463 31 AGACCAT 1655 0.0 38.066463 25 GAGACCA 1655 0.0 38.066463 24 ATCTCGT 1650 0.0 38.045456 37 ACCATTC 1650 0.0 38.045456 27 >>END_MODULE