FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_47.34100000005ea4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_47.34100000005ea4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57004
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC1354723.764998947442283RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT10301.8068907445091573No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG5881.0315065609430918No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA5170.9069538979720722No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACATC4910.8613430636446565No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC4520.792926812153533No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACA4350.7631043435548382No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT4320.757841554978598RNA PCR Primer, Index 18 (95% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC3590.6297803662900849No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC3350.5876780576801628No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC2200.385937828924286No Hit
CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG1990.34909830889060417No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.34909830889060417No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC1760.3087502631394288No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG1700.2982246859869483No Hit
TCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATC1680.2947161602694548No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGACTGTCTCTTATACACATC1580.2771735316819872No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACATC1420.24910532594203916No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA1340.23507122307206513No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGA1320.2315626973545716No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.2210371202020911No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTATAC1120.1964774401796365No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACA1090.19121465160339626No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGACTGTCTCTTATACACATCTC1050.18419760016840922No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1050.18419760016840922No Hit
TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA1000.17542628587467546No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAACTGTCTCTTATA990.1736720230159287No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA970.1701634972984352No Hit
TCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTC930.16314644586344818No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC920.16139218300470143No Hit
TTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATAC910.15963792014595468No Hit
TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC900.1578836572872079No Hit
ACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTATACACATC870.15262086871096764No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC860.1508666058522209No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC820.14384955441723388No Hit
ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAACTGTCTCTTATACACAT770.1350782401235001No Hit
GTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCA710.12455266297101958No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.12279840011227283No Hit
AAAGTACTCTGCGTTGATACCACTGCTTAGCAACTGTCTCTTATACACATC680.11928987439477931No Hit
TTGCTAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA680.11928987439477931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.11928987439477931No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC670.11753561153603256No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTAT660.1157813486772858No Hit
AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA660.1157813486772858No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACAC610.10701003438355203No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACATC610.10701003438355203No Hit
TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA590.10350150866605852No Hit
TTGCTAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC590.10350150866605852No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACAC580.10174724580731176No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCAAACTGTCTCTTATACACATC580.10174724580731176No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGA580.10174724580731176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15000.042.345
ATGCCGT16300.039.0644244
CCGAATC16350.038.39449733
CGAATCT16300.038.37423334
AATCTCG16250.038.21538536
CATTCCG16500.038.1818229
ATTCCGA16500.038.1818230
TCCGAAT16500.038.1818232
TCTCGTA16450.038.16109538
CTCGTAT16450.038.16109539
GTATGCC16750.038.14925442
TATGCCG16750.038.14925443
CGTATGC16700.038.12874241
GAATCTC16300.038.0981635
TCGTATG16600.038.0873540
TTCCGAA16550.038.06646331
AGACCAT16550.038.06646325
GAGACCA16550.038.06646324
ATCTCGT16500.038.04545637
ACCATTC16500.038.04545627
Produced by FastQC (version 0.11.2)