Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 36h_46.34100000005e97.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 269527 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 38 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC | 3829 | 1.4206368935208717 | RNA PCR Primer, Index 46 (95% over 21bp) |
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA | 1044 | 0.3873452381394072 | No Hit |
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT | 727 | 0.2697317893940125 | No Hit |
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT | 520 | 0.19293057838361277 | No Hit |
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC | 396 | 0.14692405584598203 | No Hit |
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA | 385 | 0.14284283207248255 | No Hit |
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG | 336 | 0.12466283526325748 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT | 304 | 0.1127901842858044 | RNA PCR Primer, Index 18 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 560 | 0.0 | 40.177082 | 45 |
ATGCCGT | 565 | 0.0 | 39.821533 | 44 |
AATCTCG | 560 | 0.0 | 38.971767 | 36 |
CTCGTAT | 580 | 0.0 | 38.791664 | 39 |
TCTCGTA | 580 | 0.0 | 38.791664 | 38 |
TCCGAAT | 570 | 0.0 | 38.682774 | 32 |
TTCCGAA | 565 | 0.0 | 38.626884 | 31 |
TATGCCG | 590 | 0.0 | 38.51552 | 43 |
TCGTATG | 590 | 0.0 | 38.51552 | 40 |
ATTCCGA | 570 | 0.0 | 38.28805 | 30 |
CCGAATC | 570 | 0.0 | 38.28805 | 33 |
CCCACGA | 595 | 0.0 | 38.19186 | 19 |
ATCTCGT | 590 | 0.0 | 38.134174 | 37 |
CGAGACC | 575 | 0.0 | 37.955116 | 23 |
CATTCCG | 570 | 0.0 | 37.89333 | 29 |
GCCCACG | 600 | 0.0 | 37.880623 | 18 |
CCACGAG | 600 | 0.0 | 37.873596 | 20 |
CGAATCT | 580 | 0.0 | 37.627914 | 34 |
ACGAGAC | 605 | 0.0 | 37.56059 | 22 |
CCGAGCC | 615 | 0.0 | 36.956707 | 14 |