FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_46.34100000005e97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_46.34100000005e97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269527
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC38291.4206368935208717RNA PCR Primer, Index 46 (95% over 21bp)
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA10440.3873452381394072No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT7270.2697317893940125No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT5200.19293057838361277No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC3960.14692405584598203No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA3850.14284283207248255No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG3360.12466283526325748No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT3040.1127901842858044RNA PCR Primer, Index 18 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5600.040.17708245
ATGCCGT5650.039.82153344
AATCTCG5600.038.97176736
CTCGTAT5800.038.79166439
TCTCGTA5800.038.79166438
TCCGAAT5700.038.68277432
TTCCGAA5650.038.62688431
TATGCCG5900.038.5155243
TCGTATG5900.038.5155240
ATTCCGA5700.038.2880530
CCGAATC5700.038.2880533
CCCACGA5950.038.1918619
ATCTCGT5900.038.13417437
CGAGACC5750.037.95511623
CATTCCG5700.037.8933329
GCCCACG6000.037.88062318
CCACGAG6000.037.87359620
CGAATCT5800.037.62791434
ACGAGAC6050.037.5605922
CCGAGCC6150.036.95670714