Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 36h_45.34100000005e8a.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 330352 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC | 3419 | 1.0349566522981546 | RNA PCR Primer, Index 46 (95% over 21bp) |
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA | 708 | 0.2143168499055553 | No Hit |
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT | 475 | 0.1437860221823994 | No Hit |
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA | 380 | 0.11502881774591951 | No Hit |
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC | 331 | 0.1001961544049983 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 440 | 0.0 | 38.861282 | 39 |
TCTCGTA | 440 | 0.0 | 38.861282 | 38 |
CATTCCG | 425 | 0.0 | 38.644722 | 29 |
ATTCCGA | 425 | 0.0 | 38.644722 | 30 |
ATCTCGT | 450 | 0.0 | 38.49767 | 37 |
CGAGCCC | 465 | 0.0 | 38.22349 | 15 |
CCCACGA | 460 | 0.0 | 38.149864 | 19 |
ACGAGAC | 460 | 0.0 | 38.149864 | 22 |
CCGAATC | 425 | 0.0 | 38.11534 | 33 |
TGCCGTC | 455 | 0.0 | 38.080383 | 45 |
CGAGACC | 440 | 0.0 | 37.838615 | 23 |
TCCGAGC | 470 | 0.0 | 37.81686 | 13 |
TCTCCGA | 465 | 0.0 | 37.75108 | 11 |
GCCCACG | 465 | 0.0 | 37.73965 | 18 |
CTCCGAG | 465 | 0.0 | 37.73965 | 12 |
CGAATCT | 430 | 0.0 | 37.67214 | 34 |
TATGCCG | 455 | 0.0 | 37.580143 | 43 |
CCACGAG | 470 | 0.0 | 37.338165 | 20 |
CCGAGCC | 480 | 0.0 | 37.02901 | 14 |
AATCTCG | 450 | 0.0 | 36.99776 | 36 |