FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_45.34100000005e8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_45.34100000005e8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330352
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC34191.0349566522981546RNA PCR Primer, Index 46 (95% over 21bp)
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA7080.2143168499055553No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT4750.1437860221823994No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA3800.11502881774591951No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC3310.1001961544049983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4400.038.86128239
TCTCGTA4400.038.86128238
CATTCCG4250.038.64472229
ATTCCGA4250.038.64472230
ATCTCGT4500.038.4976737
CGAGCCC4650.038.2234915
CCCACGA4600.038.14986419
ACGAGAC4600.038.14986422
CCGAATC4250.038.1153433
TGCCGTC4550.038.08038345
CGAGACC4400.037.83861523
TCCGAGC4700.037.8168613
TCTCCGA4650.037.7510811
GCCCACG4650.037.7396518
CTCCGAG4650.037.7396512
CGAATCT4300.037.6721434
TATGCCG4550.037.58014343
CCACGAG4700.037.33816520
CCGAGCC4800.037.0290114
AATCTCG4500.036.9977636