##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_44.34100000005e7d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 142576 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.32333632588935 38.0 38.0 38.0 32.0 38.0 2 36.34108826169902 38.0 38.0 38.0 32.0 38.0 3 36.314190326562674 38.0 38.0 38.0 32.0 38.0 4 36.40261334305914 38.0 38.0 38.0 32.0 38.0 5 36.41553276848838 38.0 38.0 38.0 32.0 38.0 6 38.3017127707328 40.0 38.0 40.0 38.0 40.0 7 38.190543990573445 40.0 38.0 40.0 38.0 40.0 8 38.17735102682078 40.0 38.0 40.0 38.0 40.0 9 38.19629530916844 40.0 38.0 40.0 38.0 40.0 10 38.15406520031422 40.0 38.0 40.0 38.0 40.0 11 38.118975143081585 40.0 38.0 40.0 38.0 40.0 12 38.06324346313545 40.0 38.0 40.0 38.0 40.0 13 38.07573504657165 40.0 38.0 40.0 38.0 40.0 14 38.01258977668051 40.0 38.0 40.0 32.0 40.0 15 38.02275277746605 40.0 38.0 40.0 32.0 40.0 16 38.02582482325216 40.0 38.0 40.0 32.0 40.0 17 38.01777297721917 40.0 38.0 40.0 32.0 40.0 18 38.02395213780721 40.0 38.0 40.0 32.0 40.0 19 38.06348193244305 40.0 38.0 40.0 32.0 40.0 20 38.049917237122656 40.0 38.0 40.0 32.0 40.0 21 38.06275951071709 40.0 38.0 40.0 32.0 40.0 22 38.077207945236225 40.0 38.0 40.0 38.0 40.0 23 38.063313601167096 40.0 38.0 40.0 32.0 40.0 24 38.04549152732578 40.0 38.0 40.0 32.0 40.0 25 38.04443945685108 40.0 38.0 40.0 32.0 40.0 26 37.97287060935922 40.0 38.0 40.0 32.0 40.0 27 37.940158231399394 40.0 38.0 40.0 32.0 40.0 28 37.919951464482104 40.0 38.0 40.0 32.0 40.0 29 37.904212490180676 40.0 38.0 40.0 32.0 40.0 30 37.89986393221861 40.0 38.0 40.0 32.0 40.0 31 37.88809477050836 40.0 38.0 40.0 32.0 40.0 32 37.883339411962744 40.0 38.0 40.0 32.0 40.0 33 37.83772864998317 40.0 38.0 40.0 32.0 40.0 34 37.85209291886432 40.0 38.0 40.0 32.0 40.0 35 37.82857563685333 40.0 38.0 40.0 32.0 40.0 36 37.75791858377286 40.0 38.0 40.0 32.0 40.0 37 37.789578891257996 40.0 38.0 40.0 32.0 40.0 38 37.77919846257435 40.0 38.0 40.0 32.0 40.0 39 37.73587420042644 40.0 38.0 40.0 32.0 40.0 40 37.700272135562784 40.0 38.0 40.0 32.0 40.0 41 37.70889911345528 40.0 38.0 40.0 32.0 40.0 42 37.68377567051958 40.0 38.0 40.0 32.0 40.0 43 37.66514700931433 40.0 38.0 40.0 32.0 40.0 44 37.61460554371002 40.0 38.0 40.0 32.0 40.0 45 37.57145662664123 40.0 38.0 40.0 32.0 40.0 46 37.51101868477163 40.0 38.0 40.0 32.0 40.0 47 37.485341151385924 40.0 38.0 40.0 32.0 40.0 48 37.43163646055437 40.0 38.0 40.0 32.0 40.0 49 37.38829115699697 40.0 38.0 40.0 32.0 40.0 50 37.33858433396925 40.0 38.0 40.0 32.0 40.0 51 36.92863455279991 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.49715347059120774 2201 2 0.6726115263815728 2201 3 0.39527690993555353 2201 4 0.3936916488675024 2201 5 0.5467332187871037 2201 6 1.0102628454884268 2201 7 0.9727289043203555 2201 8 0.8350786557405954 2201 9 0.4939642122267003 2201 10 0.5706560905631193 2201 11 0.6188211860904715 2201 12 0.6575662164110625 2201 13 0.868290553340124 2201 14 0.5709136630431502 2201 15 0.5250059338680728 2201 16 1.0990215355452762 2201 17 0.8798666213949389 2201 18 0.9472112788026408 2201 19 0.6543936446283922 2201 20 0.528186830328849 2201 21 0.2720759198275502 2201 22 0.6012743911638836 2201 23 0.7594059360366288 2201 24 0.678157678656099 2201 25 0.7573192875145764 2201 26 0.47494416993661304 2201 27 0.574475978462047 2201 28 0.5001860080444445 2201 29 0.5053371226358152 2201 30 0.33774848030471816 2201 31 0.9330153378887829 2201 32 0.62513391352811 2201 33 0.7773580790128563 2201 34 0.7102789676646495 2201 35 0.5163463360668317 2201 36 0.7440182829455111 2201 37 0.8272941797817737 2201 38 0.32575351370439876 2201 39 0.6951977335611517 2201 40 0.6727825938180843 2201 41 0.7581465438420025 2201 42 0.7373277618510414 2201 43 0.5460710880988202 2201 44 0.3822882036668247 2201 45 0.8068052949329498 2201 46 0.5399127746334784 2201 47 0.5160975435978656 2201 48 0.6656189741896057 2201 49 0.5928219937457655 2201 50 0.8506129320219884 2201 51 0.7854613231870431 1101 1 0.29736035695148644 1101 2 0.35437597148279565 1101 3 0.21479318992175678 1101 4 -0.12978010129258877 1101 5 0.053998870725429526 1101 6 -0.12253849434704733 1101 7 0.07287175442625937 1101 8 0.14373340181222005 1101 9 -0.0898001343572119 1101 10 -0.00241912740644068 1101 11 0.1251016648846175 1101 12 0.31300190923407456 1101 13 0.286225630429918 1101 14 -0.11725440749524552 1101 15 -0.01427784525611031 1101 16 0.3036173682628771 1101 17 0.5458436668606836 1101 18 0.33504931633773793 1101 19 0.5685358051130933 1101 20 0.27270680471435327 1101 21 0.22961495731338744 1101 22 0.7391001140346773 1101 23 -0.032525495912537394 1101 24 0.31847096371595285 1101 25 -0.004835286151745777 1101 26 -0.5230608544464275 1101 27 0.4475364188344386 1101 28 -0.04510732725923816 1101 29 0.04974380210973095 1101 30 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quality scores pass #Quality Count 15 3.0 16 5.0 17 5.0 18 7.0 19 6.0 20 15.0 21 18.0 22 49.0 23 100.0 24 217.0 25 379.0 26 601.0 27 889.0 28 1161.0 29 1599.0 30 2106.0 31 2537.0 32 3103.0 33 3818.0 34 4753.0 35 5916.0 36 7924.0 37 11829.0 38 22447.0 39 73089.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.84777529067524 22.22744591721457 14.020475325058065 25.90430346705213 2 13.754865860073645 22.842714360862704 40.93915483079081 22.463264948272837 3 18.57746044215015 26.306671529570192 31.30540904500056 23.81045898327909 4 13.040764223992818 20.430507238244864 36.72287060935922 29.805857928403096 5 13.419509594882728 36.76775894961284 35.79634721131186 14.01638424419257 6 29.64313769498373 40.002524969139266 16.600269330041524 13.754068005835485 7 24.343508023790818 33.82827404331724 21.861323083828978 19.966894849062953 8 22.633542812254518 39.20084726742229 20.95513971495904 17.210470205364157 9 22.936539108966446 18.888171922343172 22.068230277185503 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29.05748513073729 19.386151947031756 23 19.18345303557401 29.331724834474244 29.677505330490405 21.80731679946134 24 21.75471327572663 29.539333408147233 29.92368982156885 18.782263494557288 25 19.832229828302097 30.975058915946583 29.637526652452024 19.555184603299296 26 20.708689583581744 29.64796981195598 30.115098931775304 19.528241672686974 27 19.3665132981708 31.48285826506565 29.797441364605543 19.353187072158008 28 18.979351363483335 29.108685893839077 29.698546739984288 22.2134160026933 29 19.781730445516775 29.3759117944114 29.331023454157783 21.51133430591404 30 19.333253841891523 31.027613924095025 29.73844977660567 19.900682457407783 31 19.925513410391652 29.158483896307935 31.542475591964987 19.373527101335426 32 19.59235776007182 29.534423745931992 31.2801593536079 19.593059140388284 33 19.20379306475143 29.78902480080799 29.720289529794634 21.286892604645942 34 19.743154528111322 29.56879138143867 29.81427449220065 20.873779598249353 35 21.36404443945685 29.697143979351363 29.4551677701717 19.483643811020087 36 18.969532039052854 31.47093479968578 29.927196723151162 19.6323364381102 37 19.186959937156324 31.043092806643475 29.21038603972618 20.55956121647402 38 19.262007631017845 29.504965772640556 31.115334979239144 20.117691617102455 39 19.623218493996184 29.275614409157217 29.423605655930874 21.67756144091572 40 20.096650207608572 28.66120525193581 31.689765458422176 19.55237908203344 41 19.92060374817641 28.282459881045895 30.093423858152846 21.703512512624844 42 21.259538772303895 29.002777466053193 30.121479070811358 19.616204690831555 43 18.86292223095051 28.80639097744361 32.265598698238136 20.06508809336775 44 18.67004264392324 30.78147794860285 30.031001009987655 20.51747839748625 45 18.89027606329256 28.70960049377174 31.847576029626307 20.552547413309394 46 21.086999214454046 28.14428795870273 29.85986421277073 20.908848614072497 47 19.281215368861083 28.461009721267555 29.7382411940466 22.519533715824767 48 18.64479295253058 28.580546515542586 29.83461452137807 22.94004601054876 49 21.0049377174279 28.257210189653236 29.908259454606668 20.829592638312196 50 18.410531926832004 29.074318258332397 31.48636516664796 21.028784648187635 51 18.33103032258517 28.85520492119495 29.808440943556363 23.00532381266352 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1104.0 1 620.5 2 137.0 3 204.0 4 271.0 5 270.5 6 270.0 7 346.0 8 422.0 9 356.5 10 291.0 11 533.5 12 776.0 13 1288.0 14 1800.0 15 1988.0 16 2176.0 17 1623.5 18 1071.0 19 891.5 20 712.0 21 990.0 22 1268.0 23 974.0 24 680.0 25 894.0 26 1861.5 27 2615.0 28 3495.0 29 4375.0 30 6087.0 31 7799.0 32 9371.0 33 10943.0 34 11259.0 35 11575.0 36 11268.5 37 10962.0 38 9994.0 39 9026.0 40 8485.5 41 7945.0 42 7501.0 43 7057.0 44 6896.0 45 6735.0 46 6668.0 47 6601.0 48 6364.0 49 6127.0 50 6252.0 51 6377.0 52 7354.5 53 8332.0 54 6726.0 55 5120.0 56 4685.5 57 4251.0 58 3809.0 59 3367.0 60 3141.0 61 2915.0 62 2592.0 63 2269.0 64 2025.0 65 1781.0 66 1601.5 67 1422.0 68 1205.5 69 989.0 70 848.5 71 708.0 72 627.0 73 546.0 74 437.5 75 244.5 76 160.0 77 130.5 78 101.0 79 68.0 80 35.0 81 23.5 82 12.0 83 11.0 84 10.0 85 7.5 86 5.0 87 3.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04418695993715632 2 7.013803164627988E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.002104140949388396 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0035069015823139937 17 7.013803164627988E-4 18 0.0028055212658511953 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.002104140949388396 27 0.0 28 0.0 29 0.0 30 0.002104140949388396 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0014027606329255976 48 0.0 49 0.0 50 0.0 51 0.006312422848165189 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 142576.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.44747931421125 #Duplication Level Percentage of deduplicated Percentage of total 1 90.05669181065049 71.54777159732951 2 7.421519762093956 11.79242075557939 3 1.411546734548778 3.364314899823194 4 0.4554484834497735 1.4473693587025904 5 0.2169054217632219 0.8616294504336918 6 0.13064464956453747 0.622763285627458 7 0.0809833452965474 0.45037458551701526 8 0.046430105143273115 0.2951003854341475 9 0.02225190847904447 0.15910722347315465 >10 0.12028928305162843 1.7941190346937075 >50 0.017920066836015807 0.9966451040108568 >100 0.014086991744423905 1.9360307637405698 >500 0.0026407186891380043 1.1965504556954385 >1k 0.0026407186891379856 3.5358030999392747 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC 2751 1.9294972505891592 RNA PCR Primer, Index 46 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1249 0.8760240152620357 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1056 0.7406576141847155 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 699 0.49026484120749636 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 506 0.3548984401301762 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 506 0.3548984401301762 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 335 0.2349624060150376 No Hit TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT 263 0.18446302322971608 RNA PCR Primer, Index 18 (95% over 21bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 206 0.14448434519133657 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 202 0.14167882392548536 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 201 0.14097744360902253 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 199 0.13957468297609696 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 176 0.12344293569745259 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 151 0.10590842778588261 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 151 0.10590842778588261 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 146 0.10240152620356863 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 7.013803164627988E-4 11 0.0 0.0 7.013803164627988E-4 12 0.0 0.0 7.013803164627988E-4 13 0.0 0.0 0.0014027606329255976 14 0.0 0.0 0.0021041409493883965 15 0.0 0.0 0.0021041409493883965 16 0.0 0.0 0.0028055212658511953 17 0.0 0.0 0.0028055212658511953 18 0.0 0.0 0.004208281898776793 19 0.0 0.0 0.004208281898776793 20 0.0 0.0 0.004208281898776793 21 0.0 0.0 0.007715183481090787 22 0.0 0.0 0.01192346537986758 23 0.0 0.0 0.016131747278644373 24 0.0 0.0 0.021742789810346764 25 0.0 0.0 0.021742789810346764 26 0.0 0.0 0.02945797329143755 27 0.0 0.0 0.031562114240825945 28 0.0 0.0 0.05260352373470991 29 0.0 0.0 0.08276287734261026 30 0.0 0.0 0.1157277522163618 31 0.0 0.0 0.23987206823027718 32 0.0 0.0 0.3836550331051509 33 0.0 0.0 0.550583548423297 34 0.0 0.0 0.7406576141847155 35 0.0 0.0 0.9475648075412412 36 0.0 0.0 1.2533666255190214 37 0.0 0.0 1.6019526428010324 38 0.0 0.0 2.0683705532487937 39 0.0 0.0 2.6161485804062394 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 270 0.0 42.49967 45 CTCGTAT 285 0.0 40.262844 39 TATGCCG 285 0.0 40.262844 43 TCTCGTA 285 0.0 40.262844 38 AATCTCG 285 0.0 40.262844 36 TCGTATG 285 0.0 40.262844 40 ATGCCGT 285 0.0 40.262844 44 ATTCCGA 280 0.0 40.178257 30 GTATGCC 295 0.0 39.660706 42 CTCCGAG 290 0.0 39.568657 12 CGAATCT 290 0.0 39.568657 34 CCGAATC 290 0.0 39.568657 33 CATTCCG 285 0.0 39.473377 29 ATCTCGT 295 0.0 38.898003 37 CGTATGC 295 0.0 38.898003 41 TCTCCGA 295 0.0 38.898003 11 TTCCGAA 290 0.0 38.7928 31 GACCATT 290 0.0 38.7928 26 TCCGAGC 300 0.0 38.249702 13 CGAGACC 300 0.0 38.249702 23 >>END_MODULE