FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_44.34100000005e7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_44.34100000005e7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142576
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC27511.9294972505891592RNA PCR Primer, Index 46 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.8760240152620357No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10560.7406576141847155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.49026484120749636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5060.3548984401301762No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5060.3548984401301762No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3350.2349624060150376No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT2630.18446302322971608RNA PCR Primer, Index 18 (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.14448434519133657No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.14167882392548536No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.14097744360902253No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.13957468297609696No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.12344293569745259No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.10590842778588261No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.10590842778588261No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.10240152620356863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2700.042.4996745
CTCGTAT2850.040.26284439
TATGCCG2850.040.26284443
TCTCGTA2850.040.26284438
AATCTCG2850.040.26284436
TCGTATG2850.040.26284440
ATGCCGT2850.040.26284444
ATTCCGA2800.040.17825730
GTATGCC2950.039.66070642
CTCCGAG2900.039.56865712
CGAATCT2900.039.56865734
CCGAATC2900.039.56865733
CATTCCG2850.039.47337729
ATCTCGT2950.038.89800337
CGTATGC2950.038.89800341
TCTCCGA2950.038.89800311
TTCCGAA2900.038.792831
GACCATT2900.038.792826
TCCGAGC3000.038.24970213
CGAGACC3000.038.24970223