Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 36h_44.34100000005e7d.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 142576 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC | 2751 | 1.9294972505891592 | RNA PCR Primer, Index 46 (95% over 21bp) |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1249 | 0.8760240152620357 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1056 | 0.7406576141847155 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 699 | 0.49026484120749636 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 506 | 0.3548984401301762 | No Hit |
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 506 | 0.3548984401301762 | No Hit |
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 335 | 0.2349624060150376 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT | 263 | 0.18446302322971608 | RNA PCR Primer, Index 18 (95% over 21bp) |
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 206 | 0.14448434519133657 | No Hit |
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 202 | 0.14167882392548536 | No Hit |
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 201 | 0.14097744360902253 | No Hit |
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 199 | 0.13957468297609696 | No Hit |
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 176 | 0.12344293569745259 | No Hit |
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 151 | 0.10590842778588261 | No Hit |
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 151 | 0.10590842778588261 | No Hit |
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 146 | 0.10240152620356863 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 270 | 0.0 | 42.49967 | 45 |
CTCGTAT | 285 | 0.0 | 40.262844 | 39 |
TATGCCG | 285 | 0.0 | 40.262844 | 43 |
TCTCGTA | 285 | 0.0 | 40.262844 | 38 |
AATCTCG | 285 | 0.0 | 40.262844 | 36 |
TCGTATG | 285 | 0.0 | 40.262844 | 40 |
ATGCCGT | 285 | 0.0 | 40.262844 | 44 |
ATTCCGA | 280 | 0.0 | 40.178257 | 30 |
GTATGCC | 295 | 0.0 | 39.660706 | 42 |
CTCCGAG | 290 | 0.0 | 39.568657 | 12 |
CGAATCT | 290 | 0.0 | 39.568657 | 34 |
CCGAATC | 290 | 0.0 | 39.568657 | 33 |
CATTCCG | 285 | 0.0 | 39.473377 | 29 |
ATCTCGT | 295 | 0.0 | 38.898003 | 37 |
CGTATGC | 295 | 0.0 | 38.898003 | 41 |
TCTCCGA | 295 | 0.0 | 38.898003 | 11 |
TTCCGAA | 290 | 0.0 | 38.7928 | 31 |
GACCATT | 290 | 0.0 | 38.7928 | 26 |
TCCGAGC | 300 | 0.0 | 38.249702 | 13 |
CGAGACC | 300 | 0.0 | 38.249702 | 23 |