FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_43.34100000005e60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_43.34100000005e60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162311
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC49013.0195119246385027RNA PCR Primer, Index 46 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10900.6715502954205198No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9180.5655808910055388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.3499454750448214No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.28771925501044293No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT4440.2735489276758815RNA PCR Primer, Index 18 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.25753029677594247No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA2550.1571058030570941No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT2170.13369395789564478No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.1318456543302672No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.1244524400687569No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC1800.11089821392265467No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.10042449371884837No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6250.040.33049443
CATTCCG6150.040.2419729
ACGAGAC6150.040.2419722
CCGAATC6100.040.2029933
CCACGAG6250.039.95808420
ATTCCGA6200.039.91744230
TCTCGTA6200.039.91744238
AATCTCG6200.039.91744236
CGAGACC6150.039.87613723
GCCCACG6350.039.6831418
CCCACGA6350.039.6831419
ATGCCGT6300.039.65317544
ATCTCGT6250.039.59810337
CTCGTAT6250.039.59810339
ACCATTC6200.039.55455827
CGAATCT6200.039.55455834
CCATTCC6200.039.55455828
GTATGCC6400.039.38524642
CACGAGA6350.039.32882321
TCCGAAT6300.039.28383332
Produced by FastQC (version 0.11.2)