##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_42.34100000005e54.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 91665 Sequences flagged as poor quality 0 Sequence length 51 %GC 27 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.41654939180712 38.0 38.0 38.0 32.0 38.0 2 36.31480936017019 38.0 38.0 38.0 32.0 38.0 3 36.323034964272075 38.0 38.0 38.0 32.0 38.0 4 36.32624229531446 38.0 38.0 38.0 32.0 38.0 5 36.37959962908417 38.0 38.0 38.0 32.0 38.0 6 38.34178803250968 40.0 38.0 40.0 38.0 40.0 7 38.22182948780887 40.0 38.0 40.0 38.0 40.0 8 38.22228767795778 40.0 38.0 40.0 38.0 40.0 9 38.19356351933671 40.0 38.0 40.0 38.0 40.0 10 38.126645939017074 40.0 38.0 40.0 38.0 40.0 11 38.05677194130802 40.0 38.0 40.0 32.0 40.0 12 37.94052255495554 40.0 38.0 40.0 32.0 40.0 13 37.888179785087004 40.0 38.0 40.0 32.0 40.0 14 37.78515245731741 40.0 38.0 40.0 32.0 40.0 15 37.84241531664212 40.0 38.0 40.0 32.0 40.0 16 37.818109420171275 40.0 38.0 40.0 32.0 40.0 17 37.92270768559428 40.0 38.0 40.0 32.0 40.0 18 37.90134729722359 40.0 38.0 40.0 32.0 40.0 19 38.02286587028855 40.0 38.0 40.0 32.0 40.0 20 38.03375334096984 40.0 38.0 40.0 32.0 40.0 21 38.070168548519064 40.0 38.0 40.0 32.0 40.0 22 38.07230676921399 40.0 38.0 40.0 32.0 40.0 23 38.030502372770414 40.0 38.0 40.0 32.0 40.0 24 38.03861888397971 40.0 38.0 40.0 32.0 40.0 25 38.010931107838324 40.0 38.0 40.0 32.0 40.0 26 37.94159166530301 40.0 38.0 40.0 32.0 40.0 27 37.90901652757323 40.0 38.0 40.0 32.0 40.0 28 37.86918671248568 40.0 38.0 40.0 32.0 40.0 29 37.823967708503794 40.0 38.0 40.0 32.0 40.0 30 37.798123602247315 40.0 38.0 40.0 32.0 40.0 31 37.77259586537937 40.0 38.0 40.0 32.0 40.0 32 37.75456281023291 40.0 38.0 40.0 32.0 40.0 33 37.68555064637539 40.0 38.0 40.0 32.0 40.0 34 37.71230022364043 40.0 38.0 40.0 32.0 40.0 35 37.67495772650412 40.0 38.0 40.0 32.0 40.0 36 37.57345769923089 40.0 38.0 40.0 32.0 40.0 37 37.56082474226804 40.0 38.0 40.0 32.0 40.0 38 37.52766050291824 40.0 38.0 40.0 32.0 40.0 39 37.42488408880161 40.0 38.0 40.0 32.0 40.0 40 37.39968363061146 40.0 38.0 40.0 32.0 40.0 41 37.34058801069111 40.0 38.0 40.0 32.0 40.0 42 37.32788959799269 40.0 38.0 40.0 32.0 40.0 43 37.25305187367043 40.0 38.0 40.0 32.0 40.0 44 37.155064637538864 40.0 38.0 40.0 32.0 40.0 45 37.09047073583156 40.0 38.0 40.0 32.0 40.0 46 36.93902798232695 40.0 38.0 40.0 32.0 40.0 47 36.851448208149236 40.0 38.0 40.0 32.0 40.0 48 36.785937926144115 40.0 38.0 40.0 32.0 40.0 49 36.65905198276332 40.0 38.0 40.0 27.0 40.0 50 36.56106474663176 40.0 38.0 40.0 27.0 40.0 51 36.42401134566083 40.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.444735288823388 2201 2 0.09612440126664268 2201 3 -0.1627295584520425 2201 4 -0.21039278609784162 2201 5 0.21199944253631742 2201 6 0.12003302992095399 2201 7 0.6370845577977349 2201 8 0.3149700077749529 2201 9 0.28833754242052834 2201 10 0.6495944040331665 2201 11 0.48766589155645335 2201 12 0.25838498694306367 2201 13 -0.0763332331081088 2201 14 0.12370926311378128 2201 15 0.20629266860241557 2201 16 0.1778994988213043 2201 17 0.8563586473967462 2201 18 0.31159426851114347 2201 19 0.7618282721477314 2201 20 0.56226935996078 2201 21 0.33924303543822987 2201 22 0.5294932535851089 2201 23 0.282087766019508 2201 24 0.5475258194012156 2201 25 0.316983935669775 2201 26 0.3817271237245805 2201 27 0.106780658332994 2201 28 -0.11546613361041835 2201 29 0.17042387428298156 2201 30 0.24447847100144315 2201 31 0.33784309139605995 2201 32 0.8387252725331038 2201 33 0.8234960759760526 2201 34 0.7297078887295996 2201 35 0.9668234390570873 2201 36 0.5793094884439256 2201 37 0.35954171390756073 2201 38 0.5328171179763856 2201 39 0.7121998070097817 2201 40 0.5717451851615252 2201 41 0.4849345074614604 2201 42 0.05122643719295894 2201 43 0.4778356955640959 2201 44 -0.1541087656581439 2201 45 0.3633996403369366 2201 46 0.06435425653927496 2201 47 0.12019438447621411 2201 48 -0.06667746498720817 2201 49 0.38638581166262753 2201 50 -0.08961176837596696 2201 51 -0.6215385864133367 1101 1 0.0891618157332914 1101 2 0.43859567721906956 1101 3 0.1769868965637471 1101 4 -0.4236562410464373 1101 5 0.1702067932048692 1101 6 -0.12326469231246762 1101 7 0.2419357302764027 1101 8 0.6558287056181271 1101 9 0.6618509469760667 1101 10 0.4251772838289085 1101 11 -0.15561839252605125 1101 12 0.4261603010598449 1101 13 0.1293376665760917 1101 14 0.6769579360963789 1101 15 0.6920818240146289 1101 16 0.6334528146850431 1101 17 0.9167631357391457 1101 18 0.7963083718505217 1101 19 0.1550285677870491 1101 20 0.7638416236173029 1101 21 0.76395579496252 1101 22 0.7725222128003182 1101 23 -0.04875950939539564 1101 24 0.28581783042049835 1101 25 0.20672390744965696 1101 26 0.766730147308536 1101 27 0.8135602054673114 1101 28 0.4571334766832038 1101 29 0.04088009950638849 1101 30 0.6577688022518586 1101 31 0.16603633200615064 1101 32 0.6178020715655563 1101 33 0.9215273868676732 1101 34 0.49669035194266087 1101 35 0.5101278803659639 1101 36 0.36671794104084654 1101 37 -0.2079513990400983 1101 38 -0.5258404107476622 1101 39 -0.6721984997831996 1101 40 -0.1103753550521418 1101 41 -0.4789168665894721 1101 42 0.2653633719507411 1101 43 0.3689866731895748 1101 44 0.19655978234964522 1101 45 0.5669876266300165 1101 46 -0.08957976213975627 1101 47 -0.3585397417080287 1101 48 -0.5009985024124646 1101 49 -0.6626634391604611 1101 50 0.26992595923271523 1101 51 -0.05612838685679833 2202 1 0.5356443797324815 2202 2 -0.29230535079947373 2202 3 -0.4189279055594781 2202 4 0.07886341224926241 2202 5 0.4929911780735097 2202 6 0.26879336049946545 2202 7 -0.06539478104524221 2202 8 0.20753199124602872 2202 9 0.37924663332962183 2202 10 0.18678448667779435 2202 11 -0.12390435637743025 2202 12 0.39061639190174446 2202 13 0.1798651139993268 2202 14 -0.38042296829117817 2202 15 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0.0726762896197215 1102 1 -0.2790939122785403 1102 2 0.0596230238561688 1102 3 -0.1641069689203576 1102 4 0.15117746183604197 1102 5 0.3538727207732322 1102 6 0.48298068832315977 1102 7 0.282401362205448 1102 8 0.39899204634244967 1102 9 0.5094119225858194 1102 10 -0.1293312158015425 1102 11 -0.2031054583057994 1102 12 0.4023243808824617 1102 13 0.31485133989464487 1102 14 -0.859073106032227 1102 15 0.2944469385748647 1102 16 -0.5025412725285534 1102 17 0.41214377136368796 1102 18 0.06848132085274017 1102 19 0.38304039335984896 1102 20 -0.11679400092076264 1102 21 0.09544138254566548 1102 22 0.3015318210782212 1102 23 0.6016469946696432 1102 24 -0.0929700483673841 1102 25 0.4299315940719737 1102 26 0.8700191347438633 1102 27 -0.07021658684606535 1102 28 0.08425838429591437 1102 29 0.5216635437044701 1102 30 0.14668232775075296 1102 31 0.06718193437126985 1102 32 0.42619083727139184 1102 33 -0.4581843647953008 1102 34 0.08852331572684591 1102 35 0.5088344583408286 1102 36 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22 -0.11565432610792925 1213 23 -0.35644824342558934 1213 24 -0.5903726457699818 1213 25 -0.7811506570102722 1213 26 -0.8834440687193421 1213 27 -0.6119914786209577 1213 28 -0.2434472434097117 1213 29 -0.3508039887630616 1213 30 -0.6267375856691615 1213 31 -0.12827261108327548 1213 32 -0.6280515869710328 1213 33 -1.0875783041892362 1213 34 -0.5722991950956597 1213 35 -0.9913819918756204 1213 36 -0.4036421054167576 1213 37 -0.7127133038020048 1213 38 -0.96319232712397 1213 39 -0.7882960607588103 1213 40 -0.641525180836112 1213 41 -0.09192499667076959 1213 42 -0.3764949323465885 1213 43 -0.5078077518975306 1213 44 0.10057836539262155 1213 45 -0.0016534883525594068 1213 46 0.4116978220646601 1213 47 0.3609027670027771 1213 48 0.005435876217035229 1213 49 0.12985688604278778 1213 50 0.2591013367007733 1213 51 0.011188686313936103 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 1.0 15 1.0 16 3.0 17 4.0 18 10.0 19 21.0 20 24.0 21 27.0 22 89.0 23 133.0 24 208.0 25 338.0 26 486.0 27 664.0 28 859.0 29 1203.0 30 1462.0 31 1759.0 32 2214.0 33 2720.0 34 3245.0 35 3996.0 36 5208.0 37 7486.0 38 13518.0 39 45985.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.210618213564686 27.086811807715392 14.370055109947073 18.332514868772847 2 9.760860552900876 28.667277606859987 38.33213327223932 23.239728567999826 3 20.41018927616866 35.01554573719522 32.049309987454315 12.524954999181803 4 10.774014072983146 25.906289205258275 35.80101456390116 27.518682157857416 5 9.664539355261006 38.05923744068074 35.235913380243275 17.04030982381498 6 27.752140948017235 42.4862270223095 12.839142529864178 16.92248949980909 7 13.028964162984783 43.88916162112038 20.62073855888289 22.461135657011948 8 19.945453553700975 38.051600938198874 23.081874215894835 18.921071292205312 9 19.018163966617575 26.152839142529867 22.595319914907545 32.233676975945016 10 13.05078274150439 35.408280150548194 27.73904980090547 23.801887307041948 11 21.91396746778962 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38.49888179785087 39.576719576719576 8.827796869033982 25 10.815469372170403 39.972726776850486 38.367970326733214 10.843833524245895 26 11.569427474253796 38.32366032466399 40.04407400942573 10.062838191656484 27 10.267823051328207 41.40620738558883 39.2068946708122 9.119074892270769 28 8.172148584519718 37.61741122565865 39.34544264441172 14.864997545409917 29 9.44308078328697 37.90432553319151 39.72181312387498 12.930780559646541 30 8.843262821421947 41.02418642200234 40.37616050096549 9.756390255610224 31 9.663448426335023 38.545791741668026 42.35531555119184 9.435444280805106 32 9.246713576610484 38.971254022800416 42.1174930453281 9.664539355261006 33 8.137238858888344 40.66764850270005 38.91452571864943 12.280586919762177 34 8.766704849179076 41.02547319042165 38.4192439862543 11.788577974144983 35 11.947853597338133 41.244749904543724 38.290514372988596 8.516882125129548 36 8.15033000600011 44.83608792887143 38.379970544919004 8.633611520209458 37 8.258331969672176 44.42589865270278 38.081056019200346 9.234713358424699 38 8.387061582937871 41.0451099110893 41.3298423607702 9.23798614520264 39 9.07434680630557 40.375279550537286 38.25996836306115 12.290405280096001 40 9.077619593083512 40.199639993454426 41.503300060001095 9.219440353460973 41 9.279441444389898 40.27164130256914 38.08760159275623 12.361315660284733 42 12.303496427207767 40.58255604647357 38.29269623084056 8.821251295478099 43 8.393607156493754 41.224022254950086 41.48475426825942 8.897616320296732 44 8.135057001036381 44.16843942617138 38.10178366879398 9.594719903998254 45 8.872524954999182 40.71455844651721 40.69164893907162 9.72126765941199 46 12.509681994218077 40.06763759341079 37.05231004200076 10.37037037037037 47 9.349260895652648 40.259641084383354 36.65084819723995 13.74024982272405 48 8.700158184694267 40.32836960672012 36.82430589647085 14.147166312114765 49 12.532591501663667 40.26945944471718 37.01085474281351 10.187094310805652 50 8.513609338351607 40.9327442317133 40.19854906452844 10.355097365406644 51 8.46597789681544 40.554870664732 37.08665626602372 13.89249517242884 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4898.0 1 2722.5 2 547.0 3 1017.0 4 1487.0 5 1497.0 6 1507.0 7 2016.0 8 2525.0 9 1945.0 10 1365.0 11 2318.0 12 3271.0 13 5461.0 14 7651.0 15 7973.5 16 8296.0 17 6072.0 18 3848.0 19 3061.5 20 2275.0 21 3726.5 22 5178.0 23 3406.0 24 1634.0 25 1847.5 26 3214.5 27 4368.0 28 3176.0 29 1984.0 30 2386.5 31 2789.0 32 3336.0 33 3883.0 34 4091.5 35 4300.0 36 4048.5 37 3797.0 38 3444.5 39 3092.0 40 2805.0 41 2518.0 42 2314.0 43 2110.0 44 1993.0 45 1876.0 46 1801.0 47 1726.0 48 1667.5 49 1609.0 50 1922.5 51 2236.0 52 3539.5 53 4843.0 54 2999.0 55 1155.0 56 1013.5 57 872.0 58 750.5 59 629.0 60 562.0 61 495.0 62 395.0 63 295.0 64 248.5 65 202.0 66 175.0 67 148.0 68 114.0 69 80.0 70 67.5 71 55.0 72 39.5 73 24.0 74 20.5 75 15.0 76 13.0 77 9.5 78 6.0 79 3.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.032727867779414166 2 0.003272786777941417 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.002181857851960945 12 0.0 13 0.0 14 0.0 15 0.0 16 0.002181857851960945 17 0.0010909289259804725 18 0.003272786777941417 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0010909289259804725 27 0.0 28 0.0 29 0.0 30 0.002181857851960945 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.00436371570392189 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 91665.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.700070883524894 #Duplication Level Percentage of deduplicated Percentage of total 1 90.46846515312807 38.630098747622704 2 6.234636266379757 5.3243882101482125 3 1.3165499021310416 1.6865032242807971 4 0.5342239033262667 0.9124559415881975 5 0.3517864863774544 0.7510653952091734 6 0.23391383300672314 0.5992882350014451 7 0.13889631763729168 0.41516178260010606 8 0.06711566663261803 0.22926749780862532 9 0.054974477827577195 0.21126726900200826 >10 0.35064482224405696 2.8961345132659955 >50 0.07362280107261189 2.3100729060140575 >100 0.12947375191675614 12.934611764643462 >500 0.02030960814212596 6.459717692142069 >1k 0.02538701017765745 26.639966820673163 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4779 5.213549337260678 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4646 5.068455790105275 No Hit CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC 3189 3.4789723449517265 RNA PCR Primer, Index 46 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2906 3.170239458899253 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2316 2.526591392570774 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2087 2.276768668521246 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1223 1.3342060764741177 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1214 1.3243877161402935 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1151 1.2556591938035238 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1064 1.1607483772432226 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 989 1.0789287077946872 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 910 0.9927453226422298 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 775 0.8454699176348661 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 726 0.7920144002618229 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 716 0.7811051110020183 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 684 0.7461953853706431 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 611 0.6665575737740687 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 548 0.5978290514372988 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 481 0.5247368133966073 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 471 0.5138275241368024 No Hit GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 425 0.4636447935417008 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 415 0.452735504281896 No Hit TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT 404 0.44073528609611085 RNA PCR Primer, Index 18 (95% over 21bp) GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 393 0.4287350679103257 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 388 0.4232804232804233 No Hit ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 357 0.38946162657502864 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 343 0.374188621611302 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 337 0.3676430480554192 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA 314 0.3425516827578683 No Hit TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 312 0.3403698249059074 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 308 0.3360061092019855 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 307 0.33491518027600503 No Hit ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA 304 0.3316423934980636 No Hit GCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 291 0.31746031746031744 No Hit GATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 284 0.3098238149784542 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA 281 0.3065510282005127 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 274 0.2989145257186494 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 271 0.295641738940708 No Hit ATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 234 0.2552773686794305 No Hit CCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 222 0.24218622156766487 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA 221 0.2410952926416844 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAA 207 0.22582228767795776 No Hit GTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 206 0.2247313587519773 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 202 0.22036764304805542 No Hit ATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTTC 200 0.21818578519609447 RNA PCR Primer, Index 46 (95% over 24bp) CCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 198 0.21600392734413354 No Hit ACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAA 192 0.2094583537882507 No Hit TGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 191 0.2083674248622702 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 184 0.2007309223804069 No Hit ATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 175 0.19091256204658266 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 174 0.18982163312060218 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAA 170 0.1854579174166803 No Hit GTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 167 0.1821851306387389 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 165 0.18000327278677794 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 159 0.1734576992308951 No Hit TACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 148 0.16145748104510993 No Hit ACTCTGCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAA 144 0.15709376534118802 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAA 143 0.15600283641520754 No Hit CTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 129 0.14072983145148094 No Hit GATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 126 0.13745704467353953 No Hit TACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 124 0.13527518682157857 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 123 0.1341842578955981 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 116 0.12654775541373478 No Hit GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA 113 0.12327496863579337 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112 0.1221840397098129 No Hit TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 110 0.12000218185785196 No Hit GTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 110 0.12000218185785196 No Hit TTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 106 0.11563846615393006 No Hit GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101 0.11018382152402771 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 100 0.10909289259804723 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 100 0.10909289259804723 No Hit TGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 98 0.10691103474608629 No Hit AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 95 0.10363824796814489 No Hit TATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTT 92 0.10036546119020345 RNA PCR Primer, Index 46 (95% over 23bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0010909289259804723 16 0.0 0.0 0.0021818578519609447 17 0.0 0.0 0.0021818578519609447 18 0.0 0.0 0.003272786777941417 19 0.0 0.0 0.005454644629902362 20 0.0 0.0 0.006545573555882834 21 0.0 0.0 0.009818360333824251 22 0.0 0.0 0.012000218185785196 23 0.0 0.0 0.025091365297550863 24 0.0 0.0 0.043637157039218895 25 0.0 0.0 0.052364588447062675 26 0.0 0.0 0.08400152730049637 27 0.0 0.0 0.09927453226422299 28 0.0 0.0 0.14727540500736377 29 0.0 0.0 0.19091256204658266 30 0.0 0.0 0.24327715049364534 31 0.0 0.0 0.3883706976490482 32 0.0 0.0 0.5040091638029782 33 0.0 0.0 0.6021927671412207 34 0.0 0.0 0.7909234713358425 35 0.0 0.0 0.9349260895652648 36 0.0 0.0 1.1672939507991054 37 0.0 0.0 1.3691158021054928 38 0.0 0.0 1.6320296732667867 39 0.0 0.0 1.8763977526864124 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 420 0.0 36.964287 45 ACTGCGC 25 0.0020970288 36.0 8 TGCGCAA 25 0.0020970288 36.0 10 GCGCAAA 25 0.0020970288 36.0 11 GTATGCC 440 0.0 35.284092 42 TATGCCG 440 0.0 35.284092 43 ATGCCGT 440 0.0 35.284092 44 CGTATGC 440 0.0 35.284092 41 CTCGTAT 440 0.0 35.284092 39 CATTCCG 415 0.0 35.240963 29 ATTCCGA 415 0.0 35.240963 30 TCTCGTA 435 0.0 35.172413 38 CGAATCT 410 0.0 35.121952 34 TCCGAAT 410 0.0 35.121952 32 CCGAATC 410 0.0 35.121952 33 ACGAGAC 445 0.0 34.88764 22 TCGTATG 445 0.0 34.88764 40 TTCCGAA 420 0.0 34.82143 31 GAATCTC 420 0.0 34.82143 35 ATCTCGT 440 0.0 34.772728 37 >>END_MODULE