FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_42.34100000005e54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_42.34100000005e54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91665
Sequences flagged as poor quality0
Sequence length51
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47795.213549337260678No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46465.068455790105275No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC31893.4789723449517265RNA PCR Primer, Index 46 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29063.170239458899253No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23162.526591392570774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20872.276768668521246No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12231.3342060764741177No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12141.3243877161402935No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11511.2556591938035238No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10641.1607483772432226No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9891.0789287077946872No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9100.9927453226422298No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7750.8454699176348661No Hit
GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7260.7920144002618229No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7160.7811051110020183No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6840.7461953853706431No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6110.6665575737740687No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.5978290514372988No Hit
GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4810.5247368133966073No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4710.5138275241368024No Hit
GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4250.4636447935417008No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA4150.452735504281896No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT4040.44073528609611085RNA PCR Primer, Index 18 (95% over 21bp)
GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3930.4287350679103257No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3880.4232804232804233No Hit
ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3570.38946162657502864No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA3430.374188621611302No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.3676430480554192No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA3140.3425516827578683No Hit
TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.3403698249059074No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.3360061092019855No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA3070.33491518027600503No Hit
ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA3040.3316423934980636No Hit
GCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2910.31746031746031744No Hit
GATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.3098238149784542No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA2810.3065510282005127No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2740.2989145257186494No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2710.295641738940708No Hit
ATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2340.2552773686794305No Hit
CCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2220.24218622156766487No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA2210.2410952926416844No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAA2070.22582228767795776No Hit
GTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.2247313587519773No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.22036764304805542No Hit
ATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTTC2000.21818578519609447RNA PCR Primer, Index 46 (95% over 24bp)
CCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.21600392734413354No Hit
ACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAA1920.2094583537882507No Hit
TGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.2083674248622702No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.2007309223804069No Hit
ATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.19091256204658266No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.18982163312060218No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAA1700.1854579174166803No Hit
GTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.1821851306387389No Hit
CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.18000327278677794No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1590.1734576992308951No Hit
TACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.16145748104510993No Hit
ACTCTGCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAA1440.15709376534118802No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAA1430.15600283641520754No Hit
CTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.14072983145148094No Hit
GATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.13745704467353953No Hit
TACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.13527518682157857No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.1341842578955981No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.12654775541373478No Hit
GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA1130.12327496863579337No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.1221840397098129No Hit
TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.12000218185785196No Hit
GTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.12000218185785196No Hit
TTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.11563846615393006No Hit
GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.11018382152402771No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.10909289259804723No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.10909289259804723No Hit
TGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.10691103474608629No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.10363824796814489No Hit
TATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTT920.10036546119020345RNA PCR Primer, Index 46 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4200.036.96428745
ACTGCGC250.002097028836.08
TGCGCAA250.002097028836.010
GCGCAAA250.002097028836.011
GTATGCC4400.035.28409242
TATGCCG4400.035.28409243
ATGCCGT4400.035.28409244
CGTATGC4400.035.28409241
CTCGTAT4400.035.28409239
CATTCCG4150.035.24096329
ATTCCGA4150.035.24096330
TCTCGTA4350.035.17241338
CGAATCT4100.035.12195234
TCCGAAT4100.035.12195232
CCGAATC4100.035.12195233
ACGAGAC4450.034.8876422
TCGTATG4450.034.8876440
TTCCGAA4200.034.8214331
GAATCTC4200.034.8214335
ATCTCGT4400.034.77272837
Produced by FastQC (version 0.11.2)