FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_41.34100000005e3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_41.34100000005e3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3600
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC212358.97222222222223RNA PCR Primer, Index 46 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT1454.027777777777778RNA PCR Primer, Index 18 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT812.25No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG270.75No Hit
ATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTTC110.3055555555555556RNA PCR Primer, Index 46 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC90.25No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC90.25No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC70.19444444444444445No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC60.16666666666666669No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC60.16666666666666669TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC50.1388888888888889RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATATCGTATGCCGTC50.1388888888888889No Hit
GTATCTCATTGTGGTTTTGATTTGCATTTCTTTGATGGCCAGTGATGATGA40.1111111111111111No Hit
TATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTT40.1111111111111111RNA PCR Primer, Index 46 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATTTCGTATGCCGTC40.1111111111111111No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC40.1111111111111111No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC2000.041.6251
TGCCGTC2100.040.71428745
TATGCCG2100.040.71428743
TCTCGTA2100.040.71428738
ATGCCGT2100.040.71428744
CGTATGC2100.040.71428741
TCGTATG2100.040.71428740
CTCGTAT2100.040.71428739
ATCTCGT2050.040.60975637
AATCTCG2000.040.536
ACCATTC1950.040.38461727
CATTCCG1950.040.38461729
CCATTCC1950.040.38461728
AGACCAT1950.040.38461725
GACCATT1950.040.38461726
GAGACCA1950.040.38461724
TTCCGAA1900.040.2631631
CGAATCT1900.040.2631634
GAATCTC1900.040.2631635
ATTCCGA1900.040.2631630