Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 36h_41.34100000005e3a.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3600 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC | 2123 | 58.97222222222223 | RNA PCR Primer, Index 46 (95% over 21bp) |
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT | 145 | 4.027777777777778 | RNA PCR Primer, Index 18 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT | 81 | 2.25 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG | 27 | 0.75 | No Hit |
ATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTTC | 11 | 0.3055555555555556 | RNA PCR Primer, Index 46 (95% over 24bp) |
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC | 9 | 0.25 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC | 9 | 0.25 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC | 7 | 0.19444444444444445 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC | 6 | 0.16666666666666669 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC | 6 | 0.16666666666666669 | TruSeq Adapter, Index 12 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC | 5 | 0.1388888888888889 | RNA PCR Primer, Index 27 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATATCGTATGCCGTC | 5 | 0.1388888888888889 | No Hit |
GTATCTCATTGTGGTTTTGATTTGCATTTCTTTGATGGCCAGTGATGATGA | 4 | 0.1111111111111111 | No Hit |
TATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTT | 4 | 0.1111111111111111 | RNA PCR Primer, Index 46 (95% over 23bp) |
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATTTCGTATGCCGTC | 4 | 0.1111111111111111 | No Hit |
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC | 4 | 0.1111111111111111 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTTATAC | 200 | 0.0 | 41.625 | 1 |
TGCCGTC | 210 | 0.0 | 40.714287 | 45 |
TATGCCG | 210 | 0.0 | 40.714287 | 43 |
TCTCGTA | 210 | 0.0 | 40.714287 | 38 |
ATGCCGT | 210 | 0.0 | 40.714287 | 44 |
CGTATGC | 210 | 0.0 | 40.714287 | 41 |
TCGTATG | 210 | 0.0 | 40.714287 | 40 |
CTCGTAT | 210 | 0.0 | 40.714287 | 39 |
ATCTCGT | 205 | 0.0 | 40.609756 | 37 |
AATCTCG | 200 | 0.0 | 40.5 | 36 |
ACCATTC | 195 | 0.0 | 40.384617 | 27 |
CATTCCG | 195 | 0.0 | 40.384617 | 29 |
CCATTCC | 195 | 0.0 | 40.384617 | 28 |
AGACCAT | 195 | 0.0 | 40.384617 | 25 |
GACCATT | 195 | 0.0 | 40.384617 | 26 |
GAGACCA | 195 | 0.0 | 40.384617 | 24 |
TTCCGAA | 190 | 0.0 | 40.26316 | 31 |
CGAATCT | 190 | 0.0 | 40.26316 | 34 |
GAATCTC | 190 | 0.0 | 40.26316 | 35 |
ATTCCGA | 190 | 0.0 | 40.26316 | 30 |