H23NVBCXX l02n01 36h_40.34100000005e2d.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 36h_40.34100000005e2d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3950
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	2992	75.74683544303798	No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT	146	3.6962025316455698	Illumina Paired End PCR Primer 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	54	1.3670886075949367	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	35	0.8860759493670887	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT	31	0.7848101265822786	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	24	0.6075949367088608	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	21	0.5316455696202532	No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA	21	0.5316455696202532	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG	19	0.4810126582278481	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	9	0.2278481012658228	No Hit
CGGAGATGTGTATAAGAGACAGGCGTCAGATGTGTATAAGAGACAGCTTAT	9	0.2278481012658228	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	9	0.2278481012658228	RNA PCR Primer, Index 27 (95% over 21bp)
CGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGAC	8	0.20253164556962028	No Hit
ATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCTTC	6	0.1518987341772152	Illumina PCR Primer Index 4 (95% over 23bp)
CGGAGATGTGTATAAGAGACAGCGTCAGATGTGTATAAGAGACAGCTTATA	6	0.1518987341772152	No Hit
TATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCTT	6	0.1518987341772152	Illumina PCR Primer Index 4 (95% over 22bp)
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACT	6	0.1518987341772152	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATATCGTATGCCGTC	5	0.12658227848101267	No Hit
CTTATACACATCTACGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	5	0.12658227848101267	No Hit
GAAATAGGGGTTCCATGGACAAATACATTTGGGAAATGTTAAGGTGGAAGA	5	0.12658227848101267	No Hit
CTCGGAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA	4	0.10126582278481014	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATTTCGTATGCCGTC	4	0.10126582278481014	No Hit
ATGGAATGGAATGGAATGGAATGGAATCAACTCGATTGAAATGGAATGGAA	4	0.10126582278481014	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	350	43.071426	45
ATGCCGT	355	42.46479	44
CTTATAC	355	42.46479	1
TATGCCG	360	41.875	43
GTATGCC	365	41.301373	42
ATCTCGT	365	41.301373	37
CCTATCT	365	41.301373	34
TCTCGTA	365	41.301373	38
TATCTCG	365	41.301373	36
CGTATGC	365	41.301373	41
TCGTATG	365	41.301373	40
CGAGACT	365	41.301373	23
CTCGTAT	365	41.301373	39
CTATCTC	365	41.301373	35
ACTGCGA	360	41.25	27
GCGACCT	360	41.25	30
TGCGACC	360	41.25	29
CTGCGAC	360	41.25	28
GACTGCG	360	41.25	26
ACCTATC	360	41.25	33

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content