FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_38.34100000005e03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_38.34100000005e03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255495
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC24290.9507035362727255No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA9600.37574120824282276No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT5660.22153075402649758No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC4560.17847707391534082No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCAT3190.12485567232235464No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG2770.10841699446173116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3900.041.53665545
CTCGTAT4050.039.9982639
TATGCCG4050.039.9982643
TCTCGTA4050.039.9982638
ACGAGAC4050.039.9982622
CGAGACT3900.039.8059623
CTGCGAC3850.039.74630728
CCACGAG4100.039.51047520
TATCTCG4050.039.4427336
ACTGCGA3900.039.23674427
TGCGACC3900.039.23674429
GACTGCG3900.039.23674426
CGACCTA3900.039.22906531
GCCCACG4150.039.03444718
GCGACCT3950.038.74007430
ATCTCGT4250.038.6453837
CCGAGCC4200.038.5697514
TCGTATG4200.038.5697540
ATGCCGT4200.038.5697544
CACGAGA4250.038.1159921
Produced by FastQC (version 0.11.2)