FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_37.34100000005df5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_37.34100000005df5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277940
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC20210.7271353529538749No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12320.4432611354968698No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8290.29826581276534503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.26768367273512267No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA6130.2205511981003094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.20723897244009498No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT5740.206519392674678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.18960926818737858No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC4550.16370439663236672No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4380.15758796862632224No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3150.11333381305317694No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.11081528387421745No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.1050586457508815No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA2900.10433906598546448No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.1000215873929625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAA207.024324E-444.9996438
TGCCGTC2150.041.8601345
CTCGTAT2200.040.90876439
TGCGACC1950.040.38429329
CCCACGA2250.039.9996819
CCACGAG2250.039.9996820
TATCTCG2200.039.88604436
GCGACCT2000.039.37468730
CTGCGAC2000.039.37468728
GACTGCG2000.039.37468726
GCCCACG2300.039.13012318
CGAGCCC2300.039.13012315
CGAGACT2100.038.5711223
CGACCTA2050.038.41432631
ATCTCGT2350.038.29756537
CACGAGA2350.038.29756521
TATGCCG2350.038.29756543
ACGAGAC2350.038.29756522
ACTGCGA2100.037.499727
CTCCGAG2400.037.499712
Produced by FastQC (version 0.11.2)